Variations in host genes encoding adhesion molecules and susceptibility to falciparum malaria in India.
Swapnil Sinha,Tabish Qidwai,Kanika Kanchan,Prerna Anand,Ganga N Jha,Sudhanshu S. Pati,Sanjib Mohanty,Saroj K. Mishra,Prajesh K. Tyagi,Surya K. Sharma,Vimala Venkatesh,Saman Habib +11 more
TLDR
Interestingly, a SNP of the PECAM1 gene (rs668, exon 3, C/G) with low minor allele frequency in populations of the endemic region compared to the non-endemic region exhibited differential association with disease in these regions; the G allele was a risk factor for malaria in the endemic area, but exhibited significant association with protection from disease in theNon- endemic region.Abstract:
Host adhesion molecules play a significant role in the pathogenesis of Plasmodium falciparum malaria and changes in their structure or levels in individuals can influence the outcome of infection. The aim of this study was to investigate the association of SNPs of three adhesion molecule genes, ICAM1, PECAM1 and CD36, with severity of falciparum malaria in a malaria-endemic and a non-endemic region of India. The frequency distribution of seven selected SNPs of ICAM1, PECAM1 and CD36 was determined in 552 individuals drawn from 24 populations across India. SNP-disease association was analysed in a case-control study format. Genotyping of the population panel was performed by Sequenom mass spectroscopy and patient/control samples were genotyped by SNaPshot method. Haplotypes and linkage disequilibrium (LD) plots were generated using PHASE and Haploview, respectively. Odds-ratio (OR) for risk assessment was estimated using EpiInfo™ version 3.4. Association of the ICAM1 rs5498 (exon 6) G allele and the CD36 exon 1a A allele with increased risk of severe malaria was observed (severe versus control, OR = 1.91 and 2.66, P = 0.02 and 0.0012, respectively). The CD36 rs1334512 (-53) T allele as well as the TT genotype associated with protection from severe disease (severe versus control, TT versus GG, OR = 0.37, P = 0.004). Interestingly, a SNP of the PECAM1 gene (rs668, exon 3, C/G) with low minor allele frequency in populations of the endemic region compared to the non-endemic region exhibited differential association with disease in these regions; the G allele was a risk factor for malaria in the endemic region, but exhibited significant association with protection from disease in the non-endemic region. The data highlights the significance of variations in the ICAM1, PECAM1 and CD36 genes in the manifestation of falciparum malaria in India. The PECAM1 exon 3 SNP exhibits altered association with disease in the endemic and non-endemic region.read more
Citations
More filters
Journal ArticleDOI
Genetic polymorphisms linked to susceptibility to malaria
Adel Driss,Jacqueline M. Hibbert,Nana O. Wilson,Shareen Iqbal,Thomas V. Adamkiewicz,Jonathan K. Stiles +5 more
TL;DR: Diversity in human populations as well as environmental effects can influence the clinical heterogeneity of malaria, thus warranting further investigations with a goal of developing new interventions, therapies and better management against malaria.
Journal ArticleDOI
The host genetic diversity in malaria infection
Vitor R. R. Mendonça,Vitor R. R. Mendonça,Marilda Souza Goncalves,Marilda Souza Goncalves,Manoel Barral-Netto,Manoel Barral-Netto +5 more
TL;DR: The genetic alterations associated with erythrocytes or mediators of the immune system, which might influence malaria outcome are reviewed and polymorphisms in genes related to molecules involved in mechanisms of cytoadherence are discussed.
Journal ArticleDOI
CD36 and malaria: friends or foes? A decade of data provides some answers
TL;DR: Line of evidence indicates that a receptor other than CD36 is associated with severity, and isolates that cause severe disease primarily bind to endothelial protein C receptor (EPCR) rather than to CD36.
Journal ArticleDOI
Deletion of the APOBEC3B gene strongly impacts susceptibility to falciparum malaria
Pankaj Jha,Swapnil Sinha,Kanika Kanchan,Tabish Qidwai,Ankita Narang,Prashant Singh,Sudhanshu S. Pati,Sanjib Mohanty,Saroj Kanta Mishra,Surya K. Sharma,Shally Awasthi,Vimala Venkatesh,Sanjeev Jain,Analabha Basu,Shuhua Xu,Mitali Mukerji,Saman Habib +16 more
TL;DR: A case for malaria as a positive selection force for the APOBEC3B insertion is presented and a major role for this gene in innate immunity against malaria is suggested.
Journal ArticleDOI
Plasmodium falciparum malaria and the immunogenetics of ABO, HLA, and CD36 (platelet glycoprotein IV)
TL;DR: The role of the HLA system in malaria has been harder to verify, although HLA‐B53 and DRB1*04 may be associated with clinical outcome, HLA studies are challenged by numerous comparisons in this most polymorphic of systems, and confounded by increasingly heterogeneous populations.
References
More filters
Journal ArticleDOI
A simple salting out procedure for extracting DNA from human nucleated cells
TL;DR: A rapid, safe and inexpensive method was developed to simplify the deprotein-ization procedure that yielded quantities comparable to those obtained from phenol-chloroform extractions, rendering the entire process of RFLP analysis free of toxic materials.
Journal ArticleDOI
Severe falciparum malaria.
TL;DR: Children having pulmonary edema, shock and cerebral malaria had high case fatality rate and over all mortality was 9.9%, cerebral malaria being the commonest cause and multi-system involvement was seen in 58.4% cases of death.
Journal Article
An immunohistochemical study of the pathology of fatal malaria. Evidence for widespread endothelial activation and a potential role for intercellular adhesion molecule-1 in cerebral sequestration.
Gareth D. H. Turner,H Morrison,Margaret Jones,T M Davis,Sornchai Looareesuwan,I D Buley,K C Gatter,Chris I. Newbold,S Pukritayakamee,B Nagachinta +9 more
TL;DR: Results suggest that ICAM-1 and CD36 receptors have a role in sequestration in vivo and indicate that systemic endothelial activation is a feature of fatal malaria.
Journal ArticleDOI
A role for CD36 in the regulation of dendritic cell function
TL;DR: It is proposed that intact P. falciparum-infected erythrocytes and apoptotic cells engage similar pathways regulating DC function and may suggest strategies for modulating pathological immune responses in autoimmune diseases.
Journal ArticleDOI
Platelet-mediated clumping of Plasmodium falciparum-infected erythrocytes is a common adhesive phenotype and is associated with severe malaria.
Arnab Pain,David J. P. Ferguson,Oscar Kai,Britta C. Urban,Brett Lowe,Kevin Marsh,David J. Roberts +6 more
TL;DR: It is established that platelet-mediated clumping is strongly associated with severe malaria and Precise definition of the molecular basis of this intriguing adhesive phenotype may help to elucidate the complex pathophysiology of malaria.
Related Papers (5)
Genetic landscape of the people of India: A canvas for disease gene exploration
Samir K. Brahmachari,Partha P. Majumder,Mitali Mukerji,Saman Habib,Debasis Dash,Kunal Ray,Samira Bahl,Lalji Singh,Abhay Sharma,Susanta Roychoudhury,Giriraj R. Chandak,Kumarasamy Thangaraj,Devendra Parmar,Shantanu Sengupta,Dwaipayan Bharadwaj,Srikanta Kumar Rath,Jagmohan Singh,Ganga Nath Jha,Komal Virdi,V. R. Rao,Swapnil Sinha,Ashok K. Singh,Amit Kumar Mitra,Shrawan K. Mishra,Qadar Pasha,Sridhar Sivasubbu,Rajesh Pandey,Aradhita Baral,Prashant Singh,Amitabh Sharma,Jitender Kumar,Tsering Stobdan,Yasha Bhasin,Chitra Chauhan,Ashiq Hussain,Elyanambi Sundaramoorthy,Suchita Singh,Arun Bandyopadhyay,Krishanu Dasgupta,A. K. Reddy,Charles J. Spurgeon,M. Mohd Idris,Vinay K. Khanna,Alok Dhawan,Mohini Anand,Ravi Shankar,R. S. Bharti,Madhu Singh,Arvind P. Singh,Anwar J. Khan,Parag P. Shah,A. B. Pant,Rupinder Kaur,Kamlesh Bisht,Ashok Kumar,Victor Rajamanickam,Eugene Wilson,Antony Thangadurai,Pankaj Jha,Mahua Maulik,Neelam Makhija,Abdur Rahim,Sangeeta Sharma,Rupali Chopra,Pooja Rana,Manickam Chidambaram,Arindam Maitra,Ruchi Chawla,Suruchika Soni,Preeti Khurana,Mohamed Nadeem Khan,Sushanta Das Sutar,Amit Tuteja,K. Narayansamy,Rachna Shukla,S. Prakash,Swapna Mahurkar,K. Radha Mani,J. Hemavathi,Seema Bhaskar,Pankaj Khanna,G. S. Ramalakshmi,Shalini Mani Tripathi,Nikita Thakur,Balaram Ghosh,Ritushree Kukreti,Taruna Madan,Ranjana Verma,G. Sudheer,Anubha Mahajan,Sreenivas Chavali,Rubina Tabassum,Sandeep Grover,Meenal Gupta,Jyotsna Batra,Amrendra Kumar,Abdoulazim Nejatizadeh,Mudit Vaid,Swapan K Das,Shilpy Sharma,Mamta Sharma,Rajshekhar Chatterjee,Jinny A. Paul,Pragya Srivastava,Charu Rajput,Uma Mittal,Mridula Singh,Manoj Hariharan,S.K. Das,Keya Chaudhuri,Mainak Sengupta,Moulinath Acharya,Ashima Bhattacharyya,A Saha,Arindam Biswas,Moumita Chaki,Arnab Gupta,Saibal Mukherjee,Suddhasil Mookherjee,Ishita Chattopadhyay,Taraswi Banerjee,Meenakshi Chakravorty,Chaitali Misra,Gourish Monadal,Shiladitya Sengupta,Dipanjana Dutta De,Swati Bajaj,Ishani Deb,Arunava Banerjee,Rajdeep Chowdhury,Debalina Banerjee,Deepak Kumar,Sumit Ranjan Das,Shrish Tiwari,Anshu Bharadwaj,Ikhlak Ahmed,Sumera Parveen,Nivedita Singh,Dipayan Dasgupta,Siddharth Singh Bisht,Sangeeta Khanna,Rashmi Rajput,Biswaroop Ghosh,Naveen Kumar,Amit Kumar Chaurasia,James Kappukalayil Abraham,Amit Sinha,Vinod Scaria,Tavpritesh Sethi,A. Mandal,Arijit Mukhopadhyay +150 more