Yeast Carbon Catabolite Repression
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TLDR
It is possible in certain cases to propose a partial model of the way in which the different elements involved in catabolite repression may be integrated, and preliminary evidence suggests that Snf1 is in a dephosphorylated state under these conditions.Abstract:
Glucose and related sugars repress the transcription of genes encoding enzymes required for the utilization of alternative carbon sources; some of these genes are also repressed by other sugars such as galactose, and the process is known as catabolite repression. The different sugars produce signals which modify the conformation of certain proteins that, in turn, directly or through a regulatory cascade affect the expression of the genes subject to catabolite repression. These genes are not all controlled by a single set of regulatory proteins, but there are different circuits of repression for different groups of genes. However, the protein kinase Snf1/Cat1 is shared by the various circuits and is therefore a central element in the regulatory process. Snf1 is not operative in the presence of glucose, and preliminary evidence suggests that Snf1 is in a dephosphorylated state under these conditions. However, the enzymes that phosphorylate and dephosphorylate Snf1 have not been identified, and it is not known how the presence of glucose may affect their activity. What has been established is that Snf1 remains active in mutants lacking either the proteins Grr1/Cat80 or Hxk2 or the Glc7 complex, which functions as a protein phosphatase. One of the main roles of Snf1 is to relieve repression by the Mig1 complex, but it is also required for the operation of transcription factors such as Adr1 and possibly other factors that are still unidentified. Although our knowledge of catabolite repression is still very incomplete, it is possible in certain cases to propose a partial model of the way in which the different elements involved in catabolite repression may be integrated.read more
Citations
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Snf1 Kinases with Different β-Subunit Isoforms Play Distinct Roles in Regulating Haploid Invasive Growth
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Novel alleles of yeast hexokinase PII with distinct effects on catalytic activity and catabolite repression of SUC2.
Stefan Hohmann,Stefan Hohmann,Joris Winderickx,Johannes H. de Winde,Dirk Valckx,Philip Cobbaert,K Luyten,K Luyten,Catherine De Meirsman,José Ramos,Johan M. Thevelein +10 more
TL;DR: Using the set of six hxk2 mutants it was shown that there is a good correlation between the glucose-induced cAMP signal and in vivo hexokinase activity, arguing against an involvement of cAMP in either stage of catabolite repression.
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Jari Rautio,Kim Bundvig Barken,Juhani Lahdenperä,Antje Breitenstein,Søren Molin,Peter Neubauer +5 more
TL;DR: Sandwich hybridisation method offers a fast and convenient tool for following single key RNA species of interest in the production conditions and was succefully applied for monitoring the amounts of ribosomal RNA and mRNA with high expression level in shake flask cultivation conditions.
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