Yeast Carbon Catabolite Repression
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TLDR
It is possible in certain cases to propose a partial model of the way in which the different elements involved in catabolite repression may be integrated, and preliminary evidence suggests that Snf1 is in a dephosphorylated state under these conditions.Abstract:
Glucose and related sugars repress the transcription of genes encoding enzymes required for the utilization of alternative carbon sources; some of these genes are also repressed by other sugars such as galactose, and the process is known as catabolite repression. The different sugars produce signals which modify the conformation of certain proteins that, in turn, directly or through a regulatory cascade affect the expression of the genes subject to catabolite repression. These genes are not all controlled by a single set of regulatory proteins, but there are different circuits of repression for different groups of genes. However, the protein kinase Snf1/Cat1 is shared by the various circuits and is therefore a central element in the regulatory process. Snf1 is not operative in the presence of glucose, and preliminary evidence suggests that Snf1 is in a dephosphorylated state under these conditions. However, the enzymes that phosphorylate and dephosphorylate Snf1 have not been identified, and it is not known how the presence of glucose may affect their activity. What has been established is that Snf1 remains active in mutants lacking either the proteins Grr1/Cat80 or Hxk2 or the Glc7 complex, which functions as a protein phosphatase. One of the main roles of Snf1 is to relieve repression by the Mig1 complex, but it is also required for the operation of transcription factors such as Adr1 and possibly other factors that are still unidentified. Although our knowledge of catabolite repression is still very incomplete, it is possible in certain cases to propose a partial model of the way in which the different elements involved in catabolite repression may be integrated.read more
Citations
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Journal ArticleDOI
Two different signals regulate repression and induction of gene expression by glucose
TL;DR: It is shown that induction and repression of gene expression by glucose in yeast is regulated by two independent signals.
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Identification of flux regulation coefficients from elementary flux modes: A systems biology tool for analysis of metabolic networks.
Intawat Nookaew,Asawin Meechai,Chinae Thammarongtham,Kobkul Laoteng,Vasimon Ruanglek,Supapon Cheevadhanarak,Jens Nielsen,Sakarindr Bhumiratana,Sakarindr Bhumiratana,Sakarindr Bhumiratana +9 more
TL;DR: An approach to identify the set of elementary flux modes that operates in a given metabolic network through the use of measurements of macroscopic fluxes, that is, fluxes in and out of the cell, is described.
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Regulation of the CYB2 gene expression: transcriptional co-ordination by the Hap1p, Hap2/3/4/5p and Adr1p transcription factors
TL;DR: The results suggest that the Adr1p transcriptional activator is also required in CYB2 transcription control, which provides new data which allows a better understanding of the molecular mechanisms implicated in the co‐regulation at the transcriptional level of the genes encoding proteins involved in various aspects of oxidative metabolism.
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Evaluation of lipid production from xylose and glucose/xylose mixed sugar in various oleaginous yeasts and improvement of lipid production by UV mutagenesis
TL;DR: The results reveal the very diverse xylose-utilizing capability in the various oleaginous yeast species and strains and promising target genes to improve lipid productivity from xylOSE through gene recombination were identified.
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Deciphering how LIP2 and POX2 promoters can optimally regulate recombinant protein production in the yeast Yarrowia lipolytica.
Hosni Sassi,Frank Delvigne,Tambi Kar,Jean-Marc Nicaud,Anne Marie Crutz Le A.M.C.L. Coq,Sébastien Steels,Patrick Fickers,Patrick Fickers +7 more
TL;DR: This study found that pLIP2 is a promoter of choice as compared to pPOX2 to drive gene expression for recombinant protein production by Y. lipolytica cultures grown in media supplemented with different carbon sources.
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