Showing papers by "Catherine Hambly published in 2012"
Technische Universität München1, Chinese Academy of Sciences2, University of Aberdeen3, University of Buckingham4, École Polytechnique Fédérale de Lausanne5, Max Planck Society6, Baylor College of Medicine7, University of Colorado Denver8, University of California, San Francisco9, University of Chile10, Beth Israel Deaconess Medical Center11, Yale University12, University of Cincinnati Academic Health Center13, Pennington Biomedical Research Center14, Columbia University15, National Institutes of Health16, Roy J. and Lucille A. Carver College of Medicine17, Harvard University18
TL;DR: A consolidated view of the complexity and challenges of designing studies for measurement of energy metabolism in mouse models is presented, including a practical guide to the assessment of energy expenditure, energy intake and body composition and statistical analysis thereof.
Abstract: We present a consolidated view of the complexity and challenges of designing studies for measurement of energy metabolism in mouse models, including a practical guide to the assessment of energy expenditure, energy intake and body composition and statistical analysis thereof. We hope this guide will facilitate comparisons across studies and minimize spurious interpretations of data. We recommend that division of energy expenditure data by either body weight or lean body weight and that presentation of group effects as histograms should be replaced by plotting individual data and analyzing both group and body-composition effects using analysis of covariance (ANCOVA).
644 citations
TL;DR: Overall, mice exhibited different behavioral and physiological responses to intermittent starvation depending on the duration of treatment, and physical activity, mainly activity during the light phase was lowered suggesting an adaptation to unpredictable starvation.
43 citations
TL;DR: A role for TNFα and leptin in the non-pathological regulation of energy homeostasis is established, suggesting that signals from adipose tissue regulate PRH and might be targets for therapies that support people engaged in CR to reduce obesity.
Abstract: The causes of post-restriction hyperphagia (PRH) represent a target for drug-based therapies to prevent obesity. However, the factors causing PRH are poorly understood. We show that, in mice, the extent of PRH was independent of the time under restriction, but depended on its severity, suggesting that PRH was driven by signals from altered body composition. Signals related to fat mass were important drivers. Circulating levels of leptin and TNFα were significantly depleted following caloric restriction (CR). We experimentally repleted their levels to match those of controls, and found that in both treatment groups the level of PRH was significantly blunted. These data establish a role for TNFα and leptin in the non-pathological regulation of energy homeostasis. Signals from adipose tissue, including but not limited to leptin and TNFα, regulate PRH and might be targets for therapies that support people engaged in CR to reduce obesity.
21 citations
TL;DR: In comparison to the established hyper hydrating Cr/Gly/Glu supplement, supplement containing Cr/gly/Ala and decreased amount of Glu provides equal improvements in thermoregulatory and cardiovascular responses during exercise in the heat.
Abstract: Background: It has been shown that supplementation with creatine (Cr) and glycerol (Gly), when combined with glucose (Glu) necessary for the enhancement of Cr uptake by skeletal muscle, induces significant improvements in thermoregulatory and cardiovascular responses during exercise in the heat Purpose: To determine whether Cr/Gly-induced thermoregulatory and cardiovascular responses are maintained when the majority (~75%) of the Glu in the Cr/Gly supplement is replaced with the insulintropic agent alpha lipoic acid (Ala) Methods: 22 healthy endurance trained cyclists were randomly assigned to receive either 20 g/day (4×5 g/day) of Cr, 2 g kg -1 BM per day (4×05 g kg -1 BM per day) of Gly and 150 g/day (4×375 g/day) of Glu or 20 g/day (4×5 g/day) of Cr monohydrate, 2 g kg -1 BM per day (4×05 g kg -1 BM per day) of Gly (100 g/day (4×25 g/day) of Glu and 1000 mg/day (4×250 mg/day) of Ala for 7 days for 7 days Exercise trials were conducted pre- and post-supplementation and involved 40 min of constant-load cycling exercise at 70% O2 max by a self-paced 161 km time trial at 30°C and 70% relative humidity Results: Median and range values of TBW increased significantly by 21 (13-33) L and 18 (02-46) L in Cr/Gly/Glu and Cr/Gly/Glu/Ala groups respectively (P=003) and of BM not significantly by 18 (02-30) kg and 12 (05-21) kg in Cr/Gly/Glu and in Cr/Gly/Glu/Ala, respectively (P=075) During constant load exercise, heart rate (HR) and core temperature (Tcore) were significantly lower post-supplementation: HR was reduced on average by 33±21 beats/ min and by 48±33 beats/min (mean ± SD) and Tcore by 02±01 (mean ± SD) in the Cr/Gly/Glu and Cr/Gly/Glu/ Ala, respectively The reduction in HR and Tcore was not significantly different between the supplementation groups Conclusions: In comparison to the established hyper hydrating Cr/Gly/Glu supplement, supplement containing Cr/Gly/Ala and decreased amount of Glu provides equal improvements in thermoregulatory and cardiovascular responses during exercise in the heat
10 citations