抗原变异可使得多种致病微生物易于逃避宿主免疫应答。表达在感染红细胞表面的恶性疟原虫红细胞表面蛋白1（PfPMP1）与感染红细胞、内皮细胞、树突状细胞以及胎盘的单个或多个受体作用，在黏附及免疫逃避中起关键的作用。每个单倍体基因组var基因家族编码约60种成员，通过启动转录不同的var基因变异体为抗原变异提供了分子基础。

疟原虫var基因转换速率变化导致抗原变异[英]／Paul H, Robert P, Christodoulou Z, et al//Proc Natl Acad Sci U S A

Fish bioaccumulation and biomarkers in environmental risk assessment: a review

The fish gill is a multipurpose organ that, in addition to providing for aquatic gas exchange, plays dominant roles in osmotic and ionic regulation, acid-base regulation, and excretion of nitrogenous wastes Thus, despite the fact that all fish groups have functional kidneys, the gill epithelium is the site of many processes that are mediated by renal epithelia in terrestrial vertebrates Indeed, many of the pathways that mediate these processes in mammalian renal epithelial are expressed in the gill, and many of the extrinsic and intrinsic modulators of these processes are also found in fish endocrine tissues and the gill itself The basic patterns of gill physiology were outlined over a half century ago, but modern immunological and molecular techniques are bringing new insights into this complicated system Nevertheless, substantial questions about the evolution of these mechanisms and control remain

https://people.clas.ufl.edu/devans/files/PRVGill.pdf

The Multifunctional Fish Gill: Dominant Site of Gas Exchange, Osmoregulation, Acid-Base Regulation, and Excretion of Nitrogenous Waste

Nature And Properties Of Soils

Silver nanoparticles are nanoparticles of silver which are in the range of 1 and 100 nm in size. Silver nanoparticles have unique properties which help in molecular diagnostics, in therapies, as well as in devices that are used in several medical procedures. The major methods used for silver nanoparticle synthesis are the physical and chemical methods. The problem with the chemical and physical methods is that the synthesis is expensive and can also have toxic substances absorbed onto them. To overcome this, the biological method provides a feasible alternative. The major biological systems involved in this are bacteria, fungi, and plant extracts. The major applications of silver nanoparticles in the medical field include diagnostic applications and therapeutic applications. In most of the therapeutic applications, it is the antimicrobial property that is being majorly explored, though the anti-inflammatory property has its fair share of applications. Though silver nanoparticles are rampantly used in many medical procedures and devices as well as in various biological fields, they have their drawbacks due to nanotoxicity. This review provides a comprehensive view on the mechanism of action, production, applications in the medical field, and the health and environmental concerns that are allegedly caused due to these nanoparticles. The focus is on effective and efficient synthesis of silver nanoparticles while exploring their various prospective applications besides trying to understand the current scenario in the debates on the toxicity concerns these nanoparticles pose.

/pdf/silver-nanoparticles-mechanism-of-antimicrobial-action-3x1asgjbb2.pdf

Silver nanoparticles: mechanism of antimicrobial action, synthesis, medical applications, and toxicity effects

The European Commission asked EFSA to update its previous Opinion on nickel in food and drinking water, taking into account new occurrence data, the updated benchmark dose (BMD) Guidance and newly available scientific information. More than 47,000 analytical results on the occurrence of nickel were used for calculating chronic and acute dietary exposure. An increased incidence of post-implantation loss in rats was identified as the critical effect for the risk characterisation of chronic oral exposure and a BMDL 10 of 1.3 mg Ni/kg body weight (bw) per day was selected as the reference point for the establishment of a tolerable daily intake (TDI) of 13 μg/kg bw. Eczematous flare-up reactions in the skin elicited in nickel-sensitised humans, a condition known as systemic contact dermatitis, was identified as the critical effect for the risk characterisation of acute oral exposure. A BMDL could not be derived, and therefore, the lowest-observed-adverse-effect-level of 4.3 μg Ni/kg bw was selected as the reference point. The margin of exposure (MOE) approach was applied and an MOE of 30 or higher was considered as being indicative of a low health concern. The mean lower bound (LB)/upper bound (UB) chronic dietary exposure was below or at the level of the TDI. The 95th percentile LB/UB chronic dietary exposure was below the TDI in adolescents and in all adult age groups, but generally exceeded the TDI in toddlers and in other children, as well as in infants in some surveys. This may raise a health concern in these young age groups. The MOE values for the mean UB acute dietary exposure and for the 95th percentile UB raises a health concern for nickel-sensitised individuals. The MOE values for an acute scenario regarding consumption of a glass of water on an empty stomach do not raise a health concern.

Update of the risk assessment of nickel in food and drinking water.

The uptake and distribution of cadmium in rainbow trout (Salmo gairdneri) was studied after 1 week of exposure to 109Cd in water and after a subsequent 14-week period in clean water. The major target organs, that is, gills, kidney, liver and intestine, were analyzed for 109Cd content and the subcellular distribution of cadmium determined. A successive redistribution of cadmium from the gills and intestine to the liver and kidney was observed. Sephadex G-75 chromatography of the organs revealed that most of the cadmium was found in the metallothionein-containing peak and that the amount of cadmium in this peak increased over time after the exposure. Ion-exchange chromatography on fast protein, polypeptide and polynucleotide liquid chromatography of the metallothionein-containing peaks revealed that 109Cd eluted together with copper and polaro-graphic activity. There was no evidence that other low-molecular-weight cadmium-binding proteins were present in the organs. These results indicate that the metallothionein in rainbow trout binds cadmium after the fish are exposed to the metal in water.

Subcellular distribution and binding of cadmium to metallothionein in tissues of rainbow trout after exposure to 109Cd in water

Iron is an essential element because it is a key constituent of the metalloproteins involved in cellular respiration and oxygen transport. There is no known regulated excretory mechanism for iron, and homeostasis is tightly controlled via its uptake from the diet. This study assessed in vivo intestinal iron uptake and in vitro iron absorption in a marine teleost, the European flounder Platichthys flesus. Ferric iron, in the form 59 FeCl3, was reduced to Fe 2+ by ascorbate, and the bioavailability of Fe 3+ and Fe 2+ were compared. In vivo Fe 2+ uptake was significantly greater than Fe 3+ uptake and was reduced by the iron chelator desferrioxamine. Fe 2+ was also more bioavailable than Fe 3+ in in vitro studies that assessed the temporal pattern and concentration-dependency of iron absorption. The posterior region, when compared with the anterior and mid regions of the intestine, was the preferential site for Fe 2+ uptake in vivo. In vitro iron absorption was upregulated in the posterior intestine in response to prior haemoglobin depletion of the fish, and the transport showed a Q10 value of 1.94. Iron absorption in the other segments of the intestine did not correlate with haematocrit, and Q10 values were lower. Manipulation of the luminal pH had no effect on in vitro iron absorption. The present study demonstrates that a marine teleost absorbs Fe 2+ preferentially in the posterior intestine. This occurs in spite of extremely high luminal bicarbonate concentrations recorded in vivo, which may be expected to reduce the bioavailability of divalent cations as a result of the precipitation as carbonates (e.g. FeCO3). Summary

/pdf/intestinal-iron-uptake-in-the-european-flounder-platichthys-anlnoclml5.pdf

Christer Hogstrand

Papers

Update of the risk assessment of nickel in food and drinking water.

Subcellular distribution and binding of cadmium to metallothionein in tissues of rainbow trout after exposure to 109Cd in water

Intestinal iron uptake in the European flounder (Platichthys flesus)

Scientific opinion on the appropriateness to set a group health-based guidance value for zearalenone and its modified forms

The phylogeny of teleost ZIP and ZnT zinc transporters and their tissue specific expression and response to zinc in zebrafish.