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Cornelis Maria Jacobus Sagt
Researcher at DSM
Publications - 48
Citations - 1785
Cornelis Maria Jacobus Sagt is an academic researcher from DSM. The author has contributed to research in topics: Fumaric acid & Succinic acid. The author has an hindex of 14, co-authored 48 publications receiving 1696 citations. Previous affiliations of Cornelis Maria Jacobus Sagt include Leiden University & Utrecht University.
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Journal ArticleDOI
Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88
Herman Jan Pel,Johannes H. de Winde,Johannes H. de Winde,David B. Archer,Paul S. Dyer,Gerald Hofmann,Peter J. Schaap,Geoffrey Turner,Ronald P. de Vries,Richard Albang,Kaj Albermann,Mikael Rørdam Andersen,Jannick Dyrløv Bendtsen,Jacques A.E. Benen,Marco A. van den Berg,Stefaan Breestraat,Mark X. Caddick,Roland Contreras,Michael Cornell,Pedro M. Coutinho,Etienne Danchin,Alfons J. M. Debets,Peter J. T. Dekker,Piet W.M. van Dijck,Alard Van Dijk,Lubbert Dijkhuizen,Arnold J. M. Driessen,Christophe d'Enfert,Steven Geysens,Coenie Goosen,Gert S.P. Groot,Piet W. J. de Groot,Thomas Guillemette,Bernard Henrissat,Marga Herweijer,Johannes Petrus Theodorus Wilhelmus Van Den Hombergh,Cees A. M. J. J. van den Hondel,René T. J. M. van der Heijden,Rachel M. van der Kaaij,Frans M. Klis,Harrie J. Kools,Christian P. Kubicek,Patricia Ann van Kuyk,Jürgen Lauber,Xin Lu,Marc J. E. C. van der Maarel,Rogier Meulenberg,Hildegard Henna Menke,Martin Mortimer,Jens Nielsen,Stephen G. Oliver,Maurien M.A. Olsthoorn,K. Pal,K. Pal,Noël Nicolaas Maria Elisabeth Van Peij,Arthur F. J. Ram,Ursula Rinas,Johannes Andries Roubos,Cornelis Maria Jacobus Sagt,Monika Schmoll,Jibin Sun,David W. Ussery,János Varga,Wouter Vervecken,Peter J.J. Van De Vondervoort,Holger Wedler,Han A. B. Wösten,An-Ping Zeng,Albert J. J. van Ooyen,Jaap Visser,Hein Stam +70 more
TL;DR: The filamentous fungus Aspergillus niger is widely exploited by the fermentation industry for the production of enzymes and organic acids, particularly citric acid, and the sequenced genome revealed a large number of major facilitator superfamily transporters and fungal zinc binuclear cluster transcription factors.
Journal ArticleDOI
Introduction of an N-glycosylation site increases secretion of heterologous proteins in yeasts.
Cornelis Maria Jacobus Sagt,Bertrand Kleizen,René Verwaal,M. D. M. de Jong,Wally H. Müller,A. Smits,C. Visser,Johannes Boonstra,Arie J. Verkleij,C. T. Verrips +9 more
TL;DR: The results indicate that the use of N glycosylation can significantly enhance heterologous protein secretion in Saccharomyces cerevisiae.
Journal ArticleDOI
Effective lead selection for improved protein production in Aspergillus niger based on integrated genomics
Denise Ilse Jacobs,Maurien M.A. Olsthoorn,Isabelle Maillet,Michiel Akeroyd,Stefaan Breestraat,Serge Petrus Donkers,Rob van der Hoeven,Cees A. M. J. J. van den Hondel,Rolf Kooistra,Thomas Lapointe,Hildegard Henna Menke,Rogier Meulenberg,Marijke Misset,Wally H. Müller,Noël Nicolaas Maria Elisabeth Van Peij,Arthur F. J. Ram,Sabrina Rodriguez,Marc S. Roelofs,Johannes Andries Roubos,Marcel W. E. M. van Tilborg,Arie J. Verkleij,Herman Jan Pel,Hein Stam,Cornelis Maria Jacobus Sagt +23 more
TL;DR: An integrated genomics approach was developed to determine cellular responses of A. niger to protein production in well-controlled fermentations and reduction of protein degradation through the removal of the ERAD factor doaA combined with overexpression of the oligosaccharyl transferase sttC resulted in a small increase in GUS expression.
Journal ArticleDOI
Effects of a defective ERAD pathway on growth and heterologous protein production in Aspergillus niger
Neuza D. S. P. Carvalho,Mark Arentshorst,Rolf Kooistra,Hein Stam,Cornelis Maria Jacobus Sagt,Cees A. M. J. J. van den Hondel,Arthur F. J. Ram +6 more
TL;DR: The results suggest that impairing some components of the ERAD pathway in combination with high expression levels of the heterologous protein results in higher intracellular protein levels, indicating a delay in protein degradation.
Journal ArticleDOI
Impaired secretion of a hydrophobic cutinase by Saccharomyces cerevisiae correlates with an increased association with immunoglobulin heavy-chain binding protein (BiP).
Cornelis Maria Jacobus Sagt,Wally H. Müller,Johannes Boonstra,Arie J. Verkleij,C. T. Verrips +4 more
TL;DR: Results indicate that the introduction of two exposed hydrophobic patches in cutinase results in a higher affinity for BiP which might cause the retention of this mutant cut inase in the ER.