J
James Hadfield
Researcher at University of Cambridge
Publications - 41
Citations - 8639
James Hadfield is an academic researcher from University of Cambridge. The author has contributed to research in topics: Cancer & Medicine. The author has an hindex of 21, co-authored 34 publications receiving 7202 citations. Previous affiliations of James Hadfield include AstraZeneca & John Innes Centre.
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Journal ArticleDOI
Non-invasive analysis of acquired resistance to cancer therapy by sequencing of plasma DNA
Muhammed Murtaza,Sarah-Jane Dawson,Dana W.Y. Tsui,Davina Gale,Tim Forshew,Anna M. Piskorz,Christine Parkinson,Suet-Feung Chin,Zoya Kingsbury,Alvin Wong,Francesco Marass,Sean Humphray,James Hadfield,David Bentley,Tan Min Chin,Tan Min Chin,James D. Brenton,Carlos Caldas,Nitzan Rosenfeld +18 more
TL;DR: Serial analysis of cancer genomes in plasma constitutes a new paradigm for the study of clonal evolution in human cancers, establishing proof of principle that exome-wide analysis of circulating tumour DNA could complement current invasive biopsy approaches to identify mutations associated with acquired drug resistance in advanced cancers.
Journal ArticleDOI
Noninvasive Identification and Monitoring of Cancer Mutations by Targeted Deep Sequencing of Plasma DNA
Tim Forshew,Muhammed Murtaza,Christine Parkinson,Christine Parkinson,Davina Gale,Dana W.Y. Tsui,Fiona Kaper,Sarah-Jane Dawson,Sarah-Jane Dawson,Anna M. Piskorz,Mercedes Jimenez-Linan,David Bentley,James Hadfield,Andrew May,Carlos Caldas,James D. Brenton,Nitzan Rosenfeld +16 more
TL;DR: Levels of mutant alleles reflected the clinical course of the disease and its treatment—for example, stabilized disease was associated with low allelic frequency, whereas patients at relapse exhibited a rise in frequency, and TAm-Seq will need to achieve a more sensitive detection limit to identify mutations in the plasma of patients with less advanced cancers.
Journal ArticleDOI
RNA sequencing: the teenage years
TL;DR: Advances in RNA-sequencing technologies and methods over the past decade are discussed and adaptations that are enabling a fuller understanding of RNA biology are outlined, from when and where an RNA is expressed to the structures it adopts.
Journal ArticleDOI
A comprehensive assessment of RNA-seq accuracy, reproducibility and information content by the Sequencing Quality Control Consortium
Zhenqiang Su,Paweł P. Łabaj,Sheng Li,Jean Thierry-Mieg,Danielle Thierry-Mieg,Wei Shi,Charles Wang,Gary P. Schroth,Robert Setterquist,John F. Thompson,Wendell D. Jones,Wenzhong Xiao,Wenzhong Xiao,Weihong Xu,Roderick V. Jensen,Reagan Kelly,Joshua Xu,Ana Conesa,Cesare Furlanello,Hanlin Gao,Huixiao Hong,Nadereh Jafari,Stan Letovsky,Yang Liao,Fei Lu,Edward J. Oakeley,Zhiyu Peng,Craig A. Praul,Javier Santoyo-Lopez,Andreas Scherer,Tieliu Shi,Gordon K. Smyth,Frank Staedtler,Peter Sykacek,Xin Xing Tan,E. Aubrey Thompson,Jo Vandesompele,May D. Wang,Jian Wang,Russell D. Wolfinger,Jiri Zavadil,Jiri Zavadil,Scott S. Auerbach,Wenjun Bao,Hans Binder,Thomas M. Blomquist,Murray H. Brilliant,Pierre R. Bushel,Weimin Cai,Jennifer G. Catalano,Ching-Wei Chang,Tao Chen,Geng Chen,Rong Chen,Marco Chierici,Tzu Ming Chu,Djork-Arné Clevert,Youping Deng,Adnan Derti,Viswanath Devanarayan,Zirui Dong,Joaquín Dopazo,Tingting Du,Hong Fang,Yongxiang Fang,Mario Fasold,Anita Fernandez,Matthias Fischer,Pedro Furió-Tarí,James C. Fuscoe,Florian Caimet,Stan Gaj,Jorge Gandara,Huan Gao,Weigong Ge,Yoichi Gondo,Binsheng Gong,Meihua Gong,Zhuolin Gong,Bridgett Green,Chao Guo,Lei Guo,Li Wu Guo,James Hadfield,Jan Hellemans,Sepp Hochreiter,Meiwen Jia,Min Jian,Charles D. Johnson,Suzanne Kay,Jos C. S. Kleinjans,Samir Lababidi,Shawn Levy,Quan Zhen Li,Li Li,Peng Li,Yan Li,Haiqing Li,Jianying Li,Shiyong Li,Simon Lin,Francisco Javier López,Xin Lu,Heng Luo,Xiwen Ma,Joseph Meehan,Dalila B. Megherbi,Nan Mei,Bing Mu,Baitang Ning,Akhilesh Pandey,Javier Pérez-Florido,Roger Perkins,Ryan Peters,John H. Phan,Mehdi Pirooznia,Feng Qian,Tao Qing,Lucille Rainbow,Philippe Rocca-Serra,Laure Sambourg,Susanna-Assunta Sansone,Scott Schwartz,Ruchir R. Shah,Jie Shen,Todd M. Smith,Oliver Stegle,Nancy Stralis-Pavese,Elia Stupka,Yutaka Suzuki,Lee Thomas Szkotnicki,Matthew Tinning,Bimeng Tu,Joost H.M. van Delft,Alicia Vela-Boza,Elisa Venturini,Stephen J. Walker,Liqing Wan,Wei Wang,Jinhui Wang,Jun Wang,Jun Wang,Eric D. Wieben,James C. Willey,Po Yen Wu,Jiekun Xuan,Yong Yang,Zhan Ye,Ye Yin,Ying Yu,Yate Ching Yuan,John Zhang,Ke Zhang,Wenqian Zhang,Wenwei Zhang,Yanyan Zhang,Chen Zhao,Yuanting Zheng,Yiming Zhou,Paul Zumbo,Weida Tong,David P. Kreil,David P. Kreil,Christopher E. Mason,Leming Shi +164 more
TL;DR: The complete SEQC data sets, comprising >100 billion reads, provide unique resources for evaluating RNA-seq analyses for clinical and regulatory settings, and measurement performance depends on the platform and data analysis pipeline, and variation is large for transcript-level profiling.
Journal ArticleDOI
Systematic comparison of microarray profiling, real-time PCR, and next-generation sequencing technologies for measuring differential microRNA expression.
Anna Git,Heidi Dvinge,Mali Salmon-Divon,Michelle Osborne,Claudia Kutter,James Hadfield,Paul Bertone,Carlos Caldas +7 more
TL;DR: This work analyzed three biological samples across six miRNA microarray platforms and compared their hybridization performance, and validated the results for 89 miRNAs by real-time RT-PCR and challenged the use of this assay as a "gold standard."