Mark Mackiewicz

TL;DR: The authors summarize the data produced by phase III of the Encyclopedia of DNA Elements (ENCODE) project, a resource for better understanding of the human and mouse genomes, which have produced 5,992 new experimental datasets, including systematic determinations across mouse fetal development.

TL;DR: This work used clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9-based genome editing technology to develop CRISPR epitope tagging ChIP-seq (CETCh-seq) of DNA-binding proteins, and assessed the feasibility and robustness of the technique.

TL;DR: Data and analyses from chromatin immunoprecipitation followed by high-throughput sequencing (ChIP–seq) experiments using the human HepG2 cell line are presented, providing a comprehensive catalogue of the transcription factor landscape and gene regulatory networks in these cells.

TL;DR: A collection of 1,406 highly validated immunoprecipitation- and/or immunoblotting-grade mouse monoclonal antibodies to 737 human transcription factors is generated, using an integrated production and validation pipeline.

TL;DR: These results provide the most comprehensive time-course of miRNAs as part of an integrated ENCODE reference data set for mouse embryonic development and identify the potential role of specific miRNA expression clusters in suppressing the expression of mRNAs specific to other developmental programs in the organ in which they are expressed during embryonic development.