Neutralization of SARS-CoV-2 by Destruction of the Prefusion Spike.
Jiandong Huo,Jiandong Huo,Yuguang Zhao,Jingshan Ren,D. Zhou,Helen M. E. Duyvesteyn,Helen M. Ginn,Loic Carrique,Tomas Malinauskas,Reinis R. Ruza,Pranav N.M. Shah,Tiong Kit Tan,Pramila Rijal,Naomi Coombes,Kevin R. Bewley,Julia A. Tree,Julika Radecke,Neil G. Paterson,P Supasa,Juthathip Mongkolsapaya,Juthathip Mongkolsapaya,Gavin R. Screaton,Miles W. Carroll,Miles W. Carroll,Alain Townsend,Elizabeth E. Fry,Raymond J. Owens,Raymond J. Owens,David I. Stuart +28 more
TLDR
It is found that the monoclonal antibody CR3022 binds the RBD tightly, neutralising SARS-CoV-2 and the crystal structure at 2.4 Å of the Fab/RBD complex is reported, suggesting that CR30 22 binding facilitates conversion to the fusion-incompetent post-fusion state.About:
This article is published in Cell Host & Microbe.The article was published on 2020-09-09 and is currently open access. It has received 303 citations till now. The article focuses on the topics: Epitope.read more
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Macromolecular structure determination using X-rays, neutrons and electrons: recent developments in Phenix
Dorothee Liebschner,Pavel V. Afonine,Matthew L. Baker,Gábor Bunkóczi,Vincent B. Chen,Tristan I. Croll,Bradley J. Hintze,Li-Wei Hung,Swati Jain,Airlie J. McCoy,Nigel W. Moriarty,Robert D. Oeffner,Billy K. Poon,Michael G. Prisant,Randy J. Read,Jane S. Richardson,David S. Richardson,Sammito,Oleg V. Sobolev,Duncan H. Stockwell,Thomas C. Terwilliger,Alexandre Urzhumtsev,Alexandre Urzhumtsev,Lizbeth L. Videau,Carmen J. Williams,Paul D. Adams,Paul D. Adams +26 more
TL;DR: Recent developments in the Phenix software package are described in the context of macromolecular structure determination using X-rays, neutrons and electrons.
Journal ArticleDOI
Deep Mutational Scanning of SARS-CoV-2 Receptor Binding Domain Reveals Constraints on Folding and ACE2 Binding.
Tyler N. Starr,Allison J. Greaney,Allison J. Greaney,Sarah K Hilton,Sarah K Hilton,Daniel Ellis,Katharine H.D. Crawford,Katharine H.D. Crawford,Adam S. Dingens,Mary Jane Navarro,John E. Bowen,M. Alejandra Tortorici,Alexandra C. Walls,Neil P. King,David Veesler,Jesse D. Bloom,Jesse D. Bloom,Jesse D. Bloom +17 more
TL;DR: It is found that a substantial number of mutations to the RBD are well tolerated or even enhance ACE2 binding, including at ACE2 interface residues that vary across SARS-related coronaviruses.
Journal ArticleDOI
Mapping Neutralizing and Immunodominant Sites on the SARS-CoV-2 Spike Receptor-Binding Domain by Structure-Guided High-Resolution Serology.
Luca Piccoli,Young-Jun Park,M. Alejandra Tortorici,M. Alejandra Tortorici,Nadine Czudnochowski,Alexandra C. Walls,Martina Beltramello,Chiara Silacci-Fregni,Dora Pinto,Laura E. Rosen,John E. Bowen,Oliver J. Acton,Stefano Jaconi,Barbara Guarino,Andrea Minola,Fabrizia Zatta,Nicole Sprugasci,Jessica Bassi,Alessia Peter,Anna De Marco,Jay C. Nix,Federico Mele,Sandra Jovic,Blanca Fernandez Rodriguez,Sneha V. Gupta,Feng Jin,Giovanni Piumatti,Giovanni Piumatti,Giorgia Lo Presti,Alessandra Franzetti Pellanda,Maira Biggiogero,Maciej Tarkowski,Matteo Samuele Pizzuto,Elisabetta Cameroni,Colin Havenar-Daughton,Megan Smithey,David K. Hong,Valentino Lepori,Emiliano Albanese,Alessandro Ceschi,Alessandro Ceschi,Enos Bernasconi,Luigia Elzi,Paolo Ferrari,Christian Garzoni,Agostino Riva,G. Snell,Federica Sallusto,Katja Fink,Herbert W. Virgin,Antonio Lanzavecchia,Davide Corti,David Veesler +52 more
TL;DR: It is found that both the magnitude of Ab responses to SARS-CoV-2 spike (S) and nucleoprotein and nAb titers correlate with clinical scores, and the immunodominance of the receptor-binding motif will guide the design of COVID-19 vaccines and therapeutics.
Journal ArticleDOI
Complete Mapping of Mutations to the SARS-CoV-2 Spike Receptor-Binding Domain that Escape Antibody Recognition.
Allison J. Greaney,Allison J. Greaney,Tyler N. Starr,Pavlo Gilchuk,Seth J. Zost,Elad Binshtein,Andrea N. Loes,Andrea N. Loes,Sarah K Hilton,John Huddleston,Rachel Eguia,Katharine H.D. Crawford,Katharine H.D. Crawford,Adam S. Dingens,Rachel S. Nargi,Rachel E. Sutton,Naveenchandra Suryadevara,Paul W. Rothlauf,Paul W. Rothlauf,Zhuoming Liu,Sean P. J. Whelan,Robert H. Carnahan,Robert H. Carnahan,James E. Crowe,James E. Crowe,Jesse D. Bloom,Jesse D. Bloom,Jesse D. Bloom +27 more
TL;DR: A deep mutational scanning method is described to map how all amino-acid mutations in the RBD affect antibody binding, and this method is applied to 10 human monoclonal antibodies to enable rational design of antibody therapeutics and assessment of the antigenic consequences of viral evolution.
Journal ArticleDOI
SARS-CoV-2 Omicron-B.1.1.529 leads to widespread escape from neutralizing antibody responses
Wanwisa Dejnirattisai,Jiandong Huo,D. Zhou,Jiří Zahradník,P Supasa,Changxiao Liu,Helen M. E. Duyvesteyn,Helen M. Ginn,Alexander J. Mentzer,Aekkachai Tuekprakhon,Rungtiwa Nutalai,Beibei Wang,Aiste Dijokaite,Suman Khan,Ori Avinoam,M.W. Bahar,Donal T. Skelly,S Adele,Síle A. Johnson,Thomas G Ritter,Chris Jb Mason,Christina Dold,Daniel Pan,Sara Assadi,A. Bellass,Nikki Omo-Dare,David Koeckerling,Amy Flaxman,D Jenkin,Parvinder K. Aley,Merryn Voysey,Sue Ann Costa Clemens,Felipe Gomes Naveca,Valdinete Alves do Nascimento,Fernanda Nascimento,Cristiano Fernandes da Costa,Paola Cristina Resende,Alex Pauvolid-Corrêa,Marilda M. Siqueira,Vicky L. Baillie,Natali Serafin,Gaurav Kwatra,Kelly Da Silva,Shabir A. Madhi,Marta C. Nunes,Tariq Mehmood Malik,Peter J. M. Openshaw,J Kenneth Baillie,Malcolm G Semple,Alain Townsend,Kuan-Ying A. Huang,Tiong Kit Tan,Miles W. Carroll,Paul Klenerman,Eleanor Barnes,Susanna Dunachie,Bede Constantinides,Hermione J. Webster,Derrick W. Crook,Andrew J. Pollard,Teresa Lambe,Neil G. Paterson,Mark Williams,Elizabeth E. Fry,Juthathip Mongkolsapaya,Jingshan Ren,Gideon Schreiber,David Stuart,Gavin R. Screaton +68 more
TL;DR: In this article , a new SARS-CoV-2 viral isolate Omicron-B.1.529 was announced, containing far more mutations in Spike (S) than previously reported variants, leading to a large number of mutations in the ACE2 binding site and rebalances receptor affinity to that of earlier pandemic viruses.
References
More filters
Journal ArticleDOI
Coot: model-building tools for molecular graphics.
Paul Emsley,Kevin Cowtan +1 more
TL;DR: CCP4mg is a project that aims to provide a general-purpose tool for structural biologists, providing tools for X-ray structure solution, structure comparison and analysis, and publication-quality graphics.
Journal ArticleDOI
Phaser crystallographic software
Airlie J. McCoy,Ralf W. Grosse-Kunstleve,Paul D. Adams,Martyn Winn,Laurent C. Storoni,Randy J. Read +5 more
TL;DR: A description is given of Phaser-2.1: software for phasing macromolecular crystal structures by molecular replacement and single-wavelength anomalous dispersion phasing.
Journal ArticleDOI
Genomic characterisation and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding.
Roujian Lu,Xiang Zhao,Juan Li,Peihua Niu,Bo Yang,Honglong Wu,Wenling Wang,Hao Song,Baoying Huang,Na Zhu,Yuhai Bi,Xuejun Ma,Faxian Zhan,Liang Wang,Tao Hu,Hong Zhou,Zhenhong Hu,Weimin Zhou,Li Zhao,Jing Chen,Yao Meng,Ji Wang,Yang Lin,Jianying Yuan,Zhihao Xie,Jinmin Ma,William J. Liu,Dayan Wang,Wenbo Xu,Edward C. Holmes,George F. Gao,George F. Gao,Guizhen Wu,Weijun Chen,Weifeng Shi,Wenjie Tan,Wenjie Tan +36 more
TL;DR: The phylogenetic analysis suggests that bats might be the original host of this virus, an animal sold at the seafood market in Wuhan might represent an intermediate host facilitating the emergence of the virus in humans.
Journal ArticleDOI
Inference of macromolecular assemblies from crystalline state.
E. Krissinel,Kim Henrick +1 more
TL;DR: A new method, based on chemical thermodynamics, is developed for automatic detection of macromolecular assemblies in the Protein Data Bank (PDB) entries that are the results of X-ray diffraction experiments, as found, biological units may be recovered at 80-90% success rate, which makesX-ray crystallography an important source of experimental data on macromolescular complexes and protein-protein interactions.
Journal ArticleDOI
Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation.
Daniel Wrapp,Nianshuang Wang,Kizzmekia S. Corbett,Jory A. Goldsmith,Ching-Lin Hsieh,Olubukola M. Abiona,Barney S. Graham,Jason S. McLellan +7 more
TL;DR: The authors show that this protein binds at least 10 times more tightly than the corresponding spike protein of severe acute respiratory syndrome (SARS)–CoV to their common host cell receptor, and test several published SARS-CoV RBD-specific monoclonal antibodies found that they do not have appreciable binding to 2019-nCoV S, suggesting that antibody cross-reactivity may be limited between the two RBDs.
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