National Chung Hsing University

About: National Chung Hsing University is a education organization based out in Taichung, Taiwan. It is known for research contribution in the topics: Catalysis & Thin film. The organization has 19443 authors who have published 24060 publications receiving 540154 citations. The organization is also known as: NCHU.

Discrimination of Bacillus cereus and Bacillus thuringiensis with 16S rRNA and gyrB gene based PCR primers and sequencing of their annealing sites

Abstract: Aims: To evaluate the possibility for discrimination of Bacillus cereus and B. thuringiensis using 16S rRNA and gyrB gene based PCR methods, and to obtain the sequences of the primer annealing sites so that the PCR results may be explained. Methods and Results: Based on the sequence difference in the variable region (V1) of 16S rRNA and in the gyrB gene between B. cereus and B. thuringiensis, PCR primers specific to these Bacillus spp. were designed. When these primers were used to discriminate B. cereus and B. thuringiensis, six of 82 B. cereus strains were identified as B. thuringiensis while 67 of 73 B. thuringiensis strains were identified as B. cereus. Sequence analysis of the primer annealing sites showed that there is no clear-cut difference in the V1 region of 16S rRNA, and in the gyrB gene, between B. cereus and B. thuringiensis strains. Conclusions: Although 16S rDNA based probes and gyrB gene based PCR primers have been suggested for the discrimination of B. cereus and B. thuringiensis strains, when a large number of Bacillus strains was tested, results showed that discrimination between B. cereus and B. thuringiensis is difficult. Therefore, to distinguish B. thuringiensis from B. cereus, a single feature, such as the presence of a parasporal crystal protein or cry gene, may sometimes be reliable. Significance and Impact of the Study: Discrimination between B. cereus and B. thuringiensis is a challenging debate to which this paper makes a contribution.

DT-II:Digital twin enhanced Industrial Internet reference framework towards smart manufacturing

Abstract: In this paper, the interplay and relationship between digital twin and Industrial Internet are discussed at first. The sensing/transmission network capability, which is one of the main characteristics of Industrial Internet, can be a carrier for providing digital twin with a means of data acquisition and transmission. Conversely, with the capability of high-fidelity virtual modeling and simulation computing/analysis, digital twin evolving from lifecycle management for a single product to application in production/manufacturing in the shop-floor/enterprise, can further greatly enhance the simulation computing and analysis of Industrial Internet. This paper proposes a digital twin enhanced Industrial Internet (DT-II) reference framework towards smart manufacturing. To further illustrate the reference framework, the implementation and operation mechanism of DT-II is discussed from three perspectives, including product lifecycle level, intra-enterprise level and inter-enterprise level. Finally, steam turbine is taken as an example to illustrate the application scenes from above three perspectives under the circumstance of DT-II. The differences between with and without DT-II for design and development of steam turbine are also presented.