National Chung Hsing University

About: National Chung Hsing University is a education organization based out in Taichung, Taiwan. It is known for research contribution in the topics: Catalysis & Thin film. The organization has 19443 authors who have published 24060 publications receiving 540154 citations. The organization is also known as: NCHU.

Automated high-dimensional flow cytometric data analysis

Abstract: Flow cytometry is widely used for single cell interrogation of surface and intracellular protein expression by measuring fluorescence intensity of fluorophore-conjugated reagents We focus on the recently developed procedure of Pyne et al (2009, Proceedings of the National Academy of Sciences USA 106, 8519-8524) for automated high- dimensional flow cytometric analysis called FLAME (FLow analysis with Automated Multivariate Estimation) It introduced novel finite mixture models of heavy-tailed and asymmetric distributions to identify and model cell populations in a flow cytometric sample This approach robustly addresses the complexities of flow data without the need for transformation or projection to lower dimensions It also addresses the critical task of matching cell populations across samples that enables downstream analysis It thus facilitates application of flow cytometry to new biological and clinical problems To facilitate pipelining with standard bioinformatic applications such as high-dimensional visualization, subject classification or outcome prediction, FLAME has been incorporated with the GenePattern package of the Broad Institute Thereby analysis of flow data can be approached similarly as other genomic platforms We also consider some new work that proposes a rigorous and robust solution to the registration problem by a multi-level approach that allows us to model and register cell populations simultaneously across a cohort of high-dimensional flow samples This new approach is called JCM (Joint Clustering and Matching) It enables direct and rigorous comparisons across different time points or phenotypes in a complex biological study as well as for classification of new patient samples in a more clinical setting.

The microbiome extends to subepidermal compartments of normal skin

Abstract: Commensal microbes on the skin surface influence the behavior of cells below the epidermis. We hypothesized that bacteria or their products exist below the surface epithelium and thus permit physical interaction between microbes and dermal cells. Here, to test this hypothesis, we employed multiple independent detection techniques for bacteria including qPCR, Gram-staining, immunofluorescence, and in situ hybridization. Bacteria were consistently detectable within the dermis and dermal adipose of normal human skin. Sequencing of DNA from dermis and dermal adipose tissue identified bacterial 16S rRNA reflective of a diverse and partially distinct microbial community in each skin compartment. These results show the microbiota extends within the dermis, therefore enabling physical contact between bacteria and various cells below the basement membrane. These observations show that normal commensal bacterial communities directly communicate with the host in a tissue previously thought to be sterile.

Overview of Intermetallic Sigma () Phase Precipitation in Stainless Steels

Abstract: The 𝜎 phase which exists in various series of stainless steels is a significant subject in steels science and engineering. The precipitation of the 𝜎 phase is also a widely discussed aspect of the science and technology of stainless steels. The microstructural variation, precipitation mechanism, prediction method, and effects of properties of 𝜎 phase are also of importance in academic discussions. In the first section, a brief introduction to the development and the precipitation characteristics (including morphologies and precipitation sites) of 𝜎 phase in stainless steels is presented. In the second section, the properties effect, prediction method, processing effect, elemental addition, retardation method and Thermo-Calc simulation of the 𝜎 phase in stainless steels are highlighted.

CDK1-dependent phosphorylation of EZH2 suppresses methylation of H3K27 and promotes osteogenic differentiation of human mesenchymal stem cells.

Abstract: Enhancer of zeste homologue 2 (EZH2) is the catalytic subunit of Polycomb repressive complex 2 (PRC2) and catalyses the trimethylation of histone H3 on Lys 27 (H3K27), which represses gene transcription. EZH2 enhances cancer-cell invasiveness and regulates stem cell differentiation. Here, we demonstrate that EZH2 can be phosphorylated at Thr 487 through activation of cyclin-dependent kinase 1 (CDK1). The phosphorylation of EZH2 at Thr 487 disrupted EZH2 binding with the other PRC2 components SUZ12 and EED, and thereby inhibited EZH2 methyltransferase activity, resulting in inhibition of cancer-cell invasion. In human mesenchymal stem cells, activation of CDK1 promoted mesenchymal stem cell differentiation into osteoblasts through phosphorylation of EZH2 at Thr 487. These findings define a signalling link between CDK1 and EZH2 that may have an important role in diverse biological processes, including cancer-cell invasion and osteogenic differentiation of mesenchymal stem cells.

Transition-metal-catalyzed C-S bond coupling reaction.

Abstract: Sulfur-containing molecules such as thioethers are commonly found in chemical biology, organic synthesis, and materials chemistry. While many reliable methods have been developed for preparing these compounds, harsh reaction conditions are usually required in the traditional methods. The transition metals have been applied in this field, and the palladium-catalyzed coupling of thiols with aryl halides and pseudo halides is one of the most important methods in the synthesis of thioethers. Other metals have also been used for the same purpose. Here, we summarize recent efforts in metal-catalyzed C-S bond cross-coupling reactions, focusing especially on the coupling of thiols with aryl- and vinyl halides based on different metals.