Institution
National Chung Hsing University
Education•Taichung, Taiwan•
About: National Chung Hsing University is a education organization based out in Taichung, Taiwan. It is known for research contribution in the topics: Catalysis & Thin film. The organization has 19443 authors who have published 24060 publications receiving 540154 citations. The organization is also known as: NCHU.
Topics: Catalysis, Thin film, Population, Apoptosis, Gene
Papers published on a yearly basis
Papers
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TL;DR: It is demonstrated that quercetin glucuronides inhibited proliferation through G2/M arrest of the cell cycle and induced apoptosis via caspase-3 cascade in the human lung cancer cell line NCI-H209.
Abstract: Lung cancer is the leading cause of cancer death in many developed countries, including Taiwan. Quercetin, a widely distributed bioflavonoid, is well known to induce growth inhibition in a variety of human cancer cells. Quercetin glucuronides are the main circulating metabolites after dietary supplements with quercetin in humans. However, there is little information available as to how quercetin glucuronides affect human cancer cells. We investigated the effects of quercetin glucuronides in a human lung cancer cell line NCI-H209. We checked the cell viability, cell cycle checkpoint proteins, pro- and antiapoptotic proteins, caspase-3 activity, and gene expression by flow cytometry and Western blot. The viability of cells decreased in a dose- and time-dependent manner. Cell cycle analysis revealed a significant increase of the proportion of cells in G2/M phase and subG0/G1 phase (corresponding to apoptotic cells). Moreover, quercetin glucuronides increased the expressions of cyclin B, Cdc25c-ser-216-p, and Wee1 proteins, indicating the G2/M arrest. We also demonstrated a concurrent decrease of the mitochondrial membrane potential, release of cytochrome c, up-regulation of Bax, down-regulation of Bcl-2, and activation of caspase-3, and subsequently, cleavage of poly(ADP-ribose) polymerase. In addition, quercetin glucuronide-induced apoptosis was totally blocked by the broad-spectrum caspase inhibitor benzyloxycarbonyl-Val-Ala-Asp fluoromethylketone. Taken together, we demonstrated that quercetin glucuronides inhibited proliferation through G2/M arrest of the cell cycle and induced apoptosis via caspase-3 cascade in the human lung cancer cell line NCI-H209. Delineation of the biological effects of specific major quercetin metabolites on chemotherapeutic potential or chemoprevention of human cancers warrants further investigation.
176 citations
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TL;DR: Recent discoveries on bacterial community composition obtained from dark fermentation biohydrogen production systems are described, with emphasis on the possible roles of microorganisms that co-exist with common hydrogen producers.
176 citations
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TL;DR: In this article, Ganoderma tsugae Murrill (Ganodermataceae) was available in the form of mature and baby Ling chih, mycelia and fermentation filtrate.
175 citations
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TL;DR: Overall, this DG-mimetic aging study shows that SIF effectively attenuate oxidative damage and improve parameters related to aging and Alzheimer's disease.
175 citations
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TL;DR: In this article, the authors used natural areas as stimuli to analyze the psychological and physiological responses of subjects while viewing wildland scenes and found a large degree of congruency between the psychological measures of restorativeness and the three physiological responses.
174 citations
Authors
Showing all 19519 results
Name | H-index | Papers | Citations |
---|---|---|---|
Barry Halliwell | 173 | 662 | 159518 |
Chi-Huey Wong | 129 | 1220 | 66349 |
Meilin Liu | 117 | 827 | 52603 |
Wen-Hsiung Li | 106 | 461 | 61181 |
Pan-Chyr Yang | 102 | 786 | 46731 |
David A. Case | 102 | 364 | 74066 |
Jo Shu Chang | 99 | 639 | 37487 |
Wilhelm Gruissem | 94 | 325 | 32048 |
Pi-Tai Chou | 90 | 614 | 30922 |
Liang Tong | 81 | 342 | 21752 |
Tim H M Huang | 80 | 318 | 19905 |
De-en Jiang | 80 | 338 | 20466 |
Gwo-Hshiung Tzeng | 77 | 465 | 26807 |
Jianhua Yang | 74 | 554 | 27839 |
Gow-Chin Yen | 72 | 242 | 17303 |