North Eastern Hill University

About: North Eastern Hill University is a education organization based out in Shillong, Meghalaya, India. It is known for research contribution in the topics: Population & Catalysis. The organization has 2318 authors who have published 4476 publications receiving 48894 citations.

Soil moisture effects on the activity of three entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) isolated from Meghalaya, India.

Abstract: Entomopathogenic nematodes (EPNs) are obligate parasites of insects that are widely distributed in soils throughout the world. They have great potential for use as biological control agents for insect pests. It is known that strains of Steinernema and Heterorhabditis isolated from different geographical regions exhibit differences in their ecological traits, such as infectivity, establishment, survival, reproduction, etc. A precise knowledge of these factors is therefore an essential pre-requisite for devising successful strategies to use these nematodes in biological control programmes. The present study investigated the effect of soil moisture on the activity (as measured by number of nematodes established in hosts) of three entomopathogenic nematode species (Heterorhabditis indica Poinar, Karunakar & David; Steinernema thermophilum Ganguly & Singh; Steinernema glaseri Steiner), isolated from forest soils in Meghalaya, India, under laboratory conditions. The experiments for EPNs were conducted at 25 ± 2°C (30 ± 2°C for S. thermophilum) in a sandy loam soil (85% sand, 12% silt and 3% clay, pH 6.54). Last instar larvae of wax moth, Galleria mellonella served as the experimental insect host. The soil moistures tested were 3, 4, 5, 6, 8, 10, 12, 14, 16, 18, 20, 22 and 25% (w/w). The study revealed that soil moisture has marked influences on establishment of infective juveniles of different nematode species in insect host. While, S. thermophilum showed establishment at 4% and above soil moistures, H. indica and S. glaseri showed establishment at 5% and above soil moistures. The optimum soil moisture for different nematode species were noted as: H. indica 8–18%, S. thermophilum 6–20%, and S. glaseri 8–25%. Further, a minimum of 6% soil moisture was noted to be essential for achieving 100% host mortality for all the three nematode species.

Effect of sodium salicylate and sodium deoxycholate on fibrillation of bovine serum albumin: comparison of fluorescence, SANS and DLS techniques

Abstract: The impact of biocompatible additives on the fibrillation and defibrillation of proteins provides valuable insight into the development of suitable formulations for the treatment of protein-related diseases or the storage of proteins in the laboratory. We have studied the effects of the addition of sodium deoxycholate (NaDC) and sodium salicylate (NaSal) on the fibrillation of bovine serum albumin (BSA) using fluorescence, circular dichroism, dynamic light scattering (DLS) and small angle neutron scattering (SANS). Spectroscopic studies indicate that the additives are adsorbed on the surfaces of proteins and change their secondary structures, irrespective of the sequence of addition. DLS and SANS studies show that the addition of either NaSal or NaDC to native proteins slows down or arrests the formation of fibrils. However, the additives do not defibrillate preformed fibrils when added after fibril formation. Thus, NaSal and NaDC can act as potential adjuvants for the prevention of fibril formation in BSA solutions.