Showing papers by "Paul Sabatier University published in 1995"

A three-dimensional plasma and energetic particle investigation for the wind spacecraft

Abstract: This instrument is designed to make measurements of the full three-dimensional distribution of suprathermal electrons and ions from solar wind plasma to low energy cosmic rays, with high sensitivity, wide dynamic range, good energy and angular resolution, and high time resolution. The primary scientific goals are to explore the suprathermal particle population between the solar wind and low energy cosmic rays, to study particle accleration and transport and wave-particle interactions, and to monitor particle input to and output from the Earth's magnetosphere. Three arrays, each consisting of a pair of double-ended semi-conductor telescopes each with two or three closely sandwiched passivated ion implanted silicon detectors, measure electrons and ions above ∼20 keV. One side of each telescope is covered with a thin foil which absorbs ions below 400 keV, while on the other side the incoming <400 keV electrons are swept away by a magnet so electrons and ions are cleanly separated. Higher energy electrons (up to ∼1 MeV) and ions (up to 11 MeV) are identified by the two double-ended telescopes which have a third detector. The telescopes provide energy resolution of ΔE/E≈0.3 and angular resolution of 22.5°×36°, and full 4π steradian coverage in one spin (3 s). Top-hat symmetrical spherical section electrostatic analyzers with microchannel plate detectors are used to measure ions and electrons from ∼3 eV to 30 keV. All these analyzers have either 180° or 360° fields of view in a plane, ΔE/E≈0.2, and angular resolution varying from 5.6° (near the ecliptic) to 22.5°. Full 4π steradian coverage can be obtained in one-half or one spin. A large and a small geometric factor analyzer measure ions over the wide flux range from quiet-time suprathermal levels to intense solar wind fluxes. Similarly two analyzers are used to cover the wide range of electron fluxes. Moments of the electron and ion distributions are computed on board. In addition, a Fast Particle Correlator combines electron data from the high sensitivity electron analyzer with plasma wave data from the WAVE experiment (Bougeretet al., in this volume) to study wave-particle interactions on fast time scales. The large geometric factor electron analyzer has electrostatic deflectors to steer the field of view and follow the magnetic field to enhance the correlation measurements.

Glycogen synthase kinase-3 and dorsoventral patterning in Xenopus embryos.

Abstract: Glycogen synthase kinase 3 (GSK-3) is homologous to the product of the Drosophila gene shaggy (zeste-white 3), which is required for signalling by wingless during Drosophila development. To test whether GSK-3 is also involved in vertebrate pattern formation, its role was investigated during early Xenopus development. It was found that dominant-negative GSK-3 mutants induced dorsal differentiation, whereas wild-type GSK-3 induced ventralization. These results indicate that GSK-3 is required for ventral differentiation, and suggest that dorsal differentiation may involve the suppression of GSK-3 activity by a wingless/wnt-related signal.

Clustering and relaxation in Hamiltonian long-range dynamics

Abstract: We study the dynamics of a fully coupled network of N classical rotators, which can also be viewed as a mean-field XY Heisenberg (HMF) model, in the attractive (ferromagnetic) and repulsive (antiferromagnetic) cases. The exact free energy and the spectral properties of a Vlasov-Poisson equation give hints on the values of dynamical observables and on time relaxation properties. At high energy (high temperature T) the system relaxes to Maxwellian equilibrium with vanishing magnetization, but the relaxation time to the equilibrium momentum distribution diverges with N as ${\mathit{NT}}^{2}$ in the ferromagnetic case and as ${\mathit{NT}}^{3/2}$ in the antiferromagnetic case. The N dependence of the relaxation time is suggested by an analogy of the HMF model with gravitational and charged sheets dynamics in one dimension and is verified in numerical simulations. Below the critical temperature the ferromagnetic HMF mode shows a collective phenomenon where the rotators form a drifting cluster; we argue that the drifting speed vanishes as ${\mathit{N}}^{\mathrm{\ensuremath{-}}1/2}$ but increases as one approaches the critical point (a manifestation of critical slowing down). For the antiferromagnetic HMF model a two-cluster drifting state with zero magnetization forms spontaneously at very small temperatures; at larger temperatures an initial density modulation produces this state, which relaxes very slowly. This suggests the possibility of exciting magnetized states in a mean-field antiferromagnetic system.

Biochemistry and molecular biology of lignification

Abstract: SUMMARY Lignins, which result from the dehydrogenative polymerization of cinnamyl alcohols, are complex heteropolymers deposited in the walls of specific cells of higher plants. Lignins have probably been associated to land colonization by plants but several aspects concerning their biosynthesis, structure and function are still only partially understood. This review focuses on the modern physicochemical methods of structural analysis of lignins, and on the new approaches of molecular biology and genetic engineering applied to lignification. The principles, advantages and limitations of three important analytical tools for studying lignin structure are presented. They include carbon 13 nuclear magnetic resonance, analytical pyrolysis and thioacidolysis. The use of these methods is illustrated by several examples concerning the characterization of grass lignins,‘lignin-like’materials in protection barriers of plants and lignins produced by cell suspension cultures. Our present limited knowledge of the spatio temporal deposition of lignins during cell wall differentiation including the nature of the wall components associated to lignin deposition and of the cross-links between the different wall polymers is briefly reviewed. Emphasis is placed on the phenylpropanoid pathway enzymes and their corresponding genes which are described in relation to their potential roles in the quantitative and qualitative control of lignification. Recent findings concerning the promoter sequence elements responsible for the vascular expression of some of these genes are presented. A section is devoted to the enzymes specifically involved in the synthesis of monolignols: cinnamoyl CoA reductase and cinnamyl alcohol dehydrogenase. The recent characterization of the corresponding cDNAs/genes offers new possibilities for a better understanding of the regulation of lignification. Finally, at the level of the synthesis, the potential involvement of peroxidases and laccases in the polymerization of monolignols is critically discussed. In addition to previously characterized naturally occurring lignin mutants, induced lignin mutants have been obtained during the last years through genetic engineering. Some examples include plants transformed by O-methyltransferase and cinnamyl alcohol dehydrogenase antisense constructs which exhibit modified lignins. Such strategies offer promising perspectives in gaining a better understanding of lignin metabolism and functions and represent a realistic way to improve plant biomass.

Possibility theory as a basis for qualitative decision theory

Abstract: A counterpart to von Neumann and Morgenstern' expected utility theory is proposed in the framework of possibility theory. The existence of a utility function, representing a preference ordering among possibility distributions (on the consequences of decision-maker's actions) that satisfies a series of axioms pertaining to decision-maker's behavior, is established. The obtained utility is a generalization of Wald's criterion, which is recovered in case of total ignorance; when ignorance is only partial, the utility takes into account the fact that some situations are more plausible than others. Mathematically, the qualitative utility is nothing but the necessity measure of a fuzzy event in the sense of possibility theory (a so-called Sugeno integral). The possibilistic representation of uncertainty, which only requires a linearly ordered scale, is qualitative in nature. Only max, min and order-reversing operations are used on the scale. The axioms express a risk-averse behavior of the decision maker and correspond to a pessimistic view of what may happen. The proposed qualitative utility function is currently used in flexible constraint satisfaction problems under incomplete information. It can also be used in association with possibilistic logic, which is tailored to reasoning under incomplete states of knowledge.

Hinge-bending motion in citrate synthase arising from normal mode calculations

Abstract: A normal mode analysis of the closed form of dimeric citrate synthase has been performed. The largest-amplitude collective motion predicted by this method compares well with the crystallographically observed hinge-bending motion. Such a result supports those obtained previously in the case of hinge-bending motions of smaller systems, such as lysozyme or hexokinase. Taken together, all these results suggest that low-frequency normal modes may become useful for determining a first approximation of the conformational path between the closed and open forms of these proteins. © 1995 Wiley-Liss, Inc.

The Shape of Averaged Drop Size Distributions

Abstract: The shape of averaged drop size distributions (DSD) is studied from a large sample of data (892 h) collected at several sites of various latitudes The results show that neither the hypothesis of an exponential distribution to represent rainfall with a high rain raw (R) nor the concept of equilibrium distribution arising from the various models using the parameterization of Low and List is compatible with the observations To describe the DSD two regions have to be distinguished: a small-drop region, for diameter D smaller than a threshold Dc, and a large-drop region, for diameter D larger than Dc For D

The recA gene of Streptococcus pneumoniae is part of a competence-induced operon and controls lysogenic induction.

Abstract: Summary The recently identified recA gene of the naturally transformable bacterium Streptococcus pneumoniae has been further characterized by constructing a recA null mutation and by investigating its regulation. The recA mutation has been shown to confer both DNA repair (as judged from sensitivity to u.v. and methyl methane sulphonate) and recombination deficiencies. Plasmid transformation into the recA mutant was also drastically reduced. Western blotting established that recA gene expression is increased several fold at the onset of competence for genetic transformation, increased expression was associated with the appearance of a recA-specific transcript, approximately 5.7 kb long. This transcript indicated that recA is part of a competence-inducible (cin) operon. The major (about 4.3 kb) transcript detected from non-competent cells did not include cinA, the first gene in the operon, suggesting that this gene could be specifically required at some stage in the transformation process. Detection of small amounts of the 5.7 kb polycistronic mRNA in cells treated with mitomycin C suggested that the operon could also be damage inducible. In addition, mitomycin C treatment of a recA lysogenic strain did not lead to prophage induction and cell lysis. This is unlike the situation of a recA+ lysogen. Together these results demonstrate that RecA controls lysogenic induction and suggest the existence of a SOS repair system in S. pneumoniae.

Fuzzy constraints in job-shop scheduling

Abstract: This paper proposes an extension of the constraint-based approach to job-shop scheduling, that accounts for the flexibility of temporal constraints and the uncertainty of operation durations. The set of solutions to a problem is viewed as a fuzzy set whose membership function reflects preference. This membership function is obtained by an egalitarist aggregation of local constraint-satisfaction levels. Uncertainty is qualitatively described in terms of possibility distributions. The paper formulates a simple mathematical model of job-shop scheduling under preference and uncertainty, relating it to the formal framework of constraint-satisfaction problems in artificial intelligence. A combinatorial search method that solves the problem is outlined, including fuzzy extensions of well-known look-ahead schemes.

Tissue- and cell-specific expression of a cinnamyl alcohol dehydrogenase promoter in transgenic poplar plants.

Abstract: Cinnamyl alcohol dehydrogenase (CAD) which catalyses the synthesis of the cinnamyl alcohols, the immediate precursors of lignins, from the corresponding cinnamaldehydes is considered to be a highly specific marker for lignification We have isolated and characterized a CAD genomic clone from eucalyptus, a woody species of economic importance. The full-length promoter (EuCAD, 2.5 kb) and a series of 5′ deletions were fused to the β-glucuronidase (GUS) reporter gene. These constructs were tested in a homologous transient expression system of eucalyptus protoplasts which enabled the identification of several regions involved in transcriptional control. In order to study the spatial and developmental regulation of the CAD gene, the chimeric gene fusion (EuCAD-GUS) was then transferred via Agrobacterium tumefaciens-mediated transformation into poplar, an easily transformable woody angiosperm. Quantitative fluorometric assays conducted on eight independent in vitro transformants showed that GUS activity was highest in roots followed thereafter by stems and leaves. Histochemical staining for GUS activity on both in vitro primary transformants and more mature greenhouse-grown plants indicated a specific expression in the vascular tissues of stems, roots, petioles and leaves. At the onset of xylem differentiation, GUS activity was detected in parenchyma cells differentiating between the xylem-conducting elements. After secondary growth has occurred, GUS activity was localized in xylem ray cells and parenchyma cells surrounding the lignified phloem and sclerenchyma fibers. This first characterization of a woody angiosperm CAD promoter provides functional evidence for the role of CAD in lignification and suggests that parenchyma cells expressing CAD may provide lignin precursors to the adjacent lignified elements (vessels and fibres).

Identification of binding domains on the p21Cip1 cyclin-dependent kinase inhibitor.

Abstract: Members of the recently discovered family of cyclin-dependent kinases inhibitors (CKIs) appear to play an essential regulatory role in the control of cell proliferation. To investigate the molecular basis of the interaction between these proteins and the cyclin-dependent kinases (CDKs), we performed a systematic mutagenesis of the CKI family member p21Cip1 using the alanine-scanning strategy. We have examined the interaction between in vitro translated human cdk2, cyclins A and D1, purified proliferating cell nuclear antigen (PCNA) and a set of human p21Cip1 mutants fused to glutathione S-transferase. Independent domains that are required for the interaction with cdk2 and with PCNA have been identified. The cdk2 binding domain is located in the N-terminal part of the protein, between residues 45 and 60, a region that is fully conserved in the p27Kip1 inhibitor. A PCNA binding region was localised to the C-terminus of the protein, between residues 142 and 163. These findings define protein motifs that are highly conserved between members of the CKI family and that are likely to play an essential function in the regulation of the G1/S transition.

Parabolic Harnack inequality for divergence form second order differential operators

Abstract: Old and recent results concerning Harnack inequalities for divergence form operators are reviewed. In particular, the characterization of the parabolic Harnack principle by simple geometric properties -Poincare inequality and doubling property- is discussed at length. It is shown that these two properties suffice to apply Moser’s iterative technique.

Bayesian estimation of movement and survival probabilities from capture-recapture data

Abstract: SUMMARY The Arnason-Schwarz model is usually used for estimating survival and movement probabilities of animal populations from capture-recapture data. The missing data structure of this capture-recapture model is exhibited and summarised via a directed graph representation. Taking advantage of this structure we implement a Gibbs sampling algorithm from which Bayesian estimates and credible intervals for survival and movement probabilities are derived. Convergence of the algorithm is proved using a duality principle. We illustrate our approach through a real example.

Shape anisotropy versus magnetic interactions of magnetite grains: Experiments and application to AMS in granitic rocks

Abstract: The magnetic fabric of ferromagnetic granitic rocks results from both the shape preferred orientation of individual magnetite grains and their distribution anisotropy through magnetic interactions between neighbouring grains. Measurement of the low-field magnetic anisotropy of single multi-domain magnetite grains shows a linear correlation between their magnetic anisotropy degree and their aspect ratio. Interactions between two elongated grains were studied experimentally using two types of grain arrangement: an “aligned” configuration and a “side-by-side” configuration. For a distance between the grain centers equal to approximately twice the average grain size, the magnetic susceptibility and its anisotropy are enhanced in both configurations, and the direction of kmax, the easiest magnetization axis, is stable in the “aligned” configuration, whereas it rotates toward an orthogonal direction in the “side-by-side” configuration. Depending on the distribution of the interacting magnetite grains, magnetic interactions may therefore either increase the whole-rock anisotropy magnitude, or reduce it as in the given example of the granitic rocks from Madagascar.

Phase diagram of a model of self-organizing hierarchies

Abstract: We introduce a simple model of self-organizing hierarchies in animal societies which relies on a basic positive feedback mechanism reinforcing the ability of a given individual to win or lose in a hierarchical interaction, depending on how many times it won or lost in previous interactions. If a forgetting strength is included, which determines the rate at which events in the past are forgotten and no longer influence the force of an individual, subcritical or supercritical bifurcations in the formation of the hierarchical structure are observed as the density ϱ of individuals is varied. The nature of the transition is shown to depend on a parameter η, analogous to the inverse of a temperature, defining the amount of determinism in the outcomes of the fights. We therefore observe a dynamical tricritical point in the ϱ-η plane.

Elicitation, assessment, and pooling of expert judgments using possibility theory

Abstract: The problem of modeling expert knowledge about numerical parameters in the field of reliability is reconsidered in the framework of possibility theory. Usually expert opinions about quantities such as failure rates are modeled, assessed, and pooled in the setting of probability theory. This approach does not seem to always be natural since probabilistic information looks too rich to be currently supplied by individuals. Indeed, information supplied by individuals is often incomplete, imprecise rather than tainted with randomness. Moreover, the probabilistic framework looks somewhat restrictive to express the variety of possible pooling modes. In this paper, the authors formulate a model of expert opinion by means of possibility distributions that are thought to better reflect the imprecision pervading expert judgments. They are weak substitutes to unreachable subjective probabilities. Assessment evaluation is carried out in terms of calibration and level of precision, respectively, measured by membership grades and fuzzy cardinality indexes. Finally, drawing from previous works on data fusion using possibility theory, the authors present various pooling modes with their formal model under various assumptions concerning the experts. A comparative experiment between two computerized systems for expert opinion analysis has been carried out, and its results are presented in this paper. >

A modified parameterization of flux-profile relationships in the surface layer using different roughness length values for heat and momentum

Abstract: The surface-layer flux-profile formulae of Louis (1979), used in many atmospheric models, are modified in a simple way to allow for different values of the roughness lengths for heat and momentum. The modified set of formulae simplifies the calculation of surface-layer fluxes over most natural land surfaces, where the roughness length for momentum can be almost an order of magnitude greater than that for heat.

Characterization of a binding site for chemically synthesized lipo-oligosaccharidic NodRm factors in particulate fractions prepared from roots.

Abstract: Summary This paper describes the characteristics of a binding site for the major, lipo-oligosaccharide Nod factor of Rhizobium meliloti in roots of the symbiotic host plant, Medicago truncatula. Chemically synthesized NodRm-IV(Ac, S, C16:2) was labelled by tritiation to a specific activity of 56 Ci mmol−1 and this ligand was shown to be biologically active in the root hair deformation assay at 10−11 M. Binding of the ligand to a particulate fraction from roots of M. truncatula was found to be saturable and reversible with an affinity (Kd) of 86 nM and the binding characteristics were consistent with a single class of binding sites. Competition with modified Nod factors showed that the binding was independent of both the O-acetyl and the sulphyl group and did not depend on the unsaturation of the fatty acid. However, both moieties of the lipo-oligosaccharide are required for high-affinity binding since tetra-N-acetyl-chitotetraose and palmitate were found to be poor competitors of ligand binding. A binding site with analogous characteristics was also found in a similarly prepared particulate fraction of tomato roots. This binding site for Nod factors, termed NFBS1, which is present in both a leguminous and a non-leguminous plant, may have a more general role than symbiosis.