Institution
Walter and Eliza Hall Institute of Medical Research
Nonprofit•Melbourne, Victoria, Australia•
About: Walter and Eliza Hall Institute of Medical Research is a nonprofit organization based out in Melbourne, Victoria, Australia. It is known for research contribution in the topics: Antigen & Immune system. The organization has 5012 authors who have published 10620 publications receiving 873561 citations.
Topics: Antigen, Immune system, Population, T cell, Plasmodium falciparum
Papers published on a yearly basis
Papers
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TL;DR: The three splenic DC subtypes behave as rapidly turning over products of three independent developmental streams, with the CD8α+ spleen DC, apparently the most mature, displayed an extremely rapid turnover based on bromodeoxyuridine uptake and the fastest generation from bone marrow precursors.
Abstract: Three distinct subtypes of dendritic cells (DC) are present in mouse spleen, separable as CD4(-)8alpha(-), CD4(+)8alpha(-), and CD4(-)8alpha(+) DC. We have tested whether these represent stages of development or activation within one DC lineage, or whether they represent separate DC lineages. All three DC subtypes appear relatively mature by many criteria, but all retain a capacity to phagocytose particulate material in vivo. Although further maturation or activation could be induced by bacterially derived stimuli, phagocytic capacity was retained, and no DC subtype was converted to the other. Continuous elimination of CD4(+)8(-) DC by Ab depletion had no effect on the levels of the other DC subtypes. Bromodeoxyuridine labeling experiments indicated that all three DC subtypes have a rapid turnover (half-life, 1.5-2.9 days) in the spleen, with none being the precursor of another. The three DC subtypes showed different kinetics of development from bone marrow precursors. The CD8alpha(+) spleen DC, apparently the most mature, displayed an extremely rapid turnover based on bromodeoxyuridine uptake and the fastest generation from bone marrow precursors. In conclusion, the three splenic DC subtypes behave as rapidly turning over products of three independent developmental streams.
437 citations
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TL;DR: A single species of HSPG undergoes a rapid, tightly controlled change in growth factor-binding specificity concomitant with the temporal expression of the FGFs, which is an essential prerequisite for the binding of growth factor to the signal transducing receptors.
Abstract: Murine neural precursor cells and cell lines derived from them are stimulated by members of the heparin-binding fibroblast growth factor (FGF) family. The activity of FGF is regulated by heparan sulfate proteoglycans (HSPGs), and this interaction is an essential prerequisite for the binding of growth factor to the signal transducing receptors. Messenger RNA for FGF-2 was detectable in the neuroepithelium at embryonic day 9, and the HSPGs produced by these cells at this time preferentially bound FGF-2. However, at embryonic day 11, when messenger RNA for FGF-1 was first detectable, there was a switch in the binding specificity of the HSPG to FGF-1. Thus, a single species of HSPG undergoes a rapid, tightly controlled change in growth factor-binding specificity concomitant with the temporal expression of the FGFs.
437 citations
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TL;DR: It is demonstrated that the partial deficiency in RelB null thymic DC is a secondary effect of disruptedThymic architecture, and the deficiency in splenic CD8alpha- DEC-205- DC is an direct, stem cell intrinsic effect of the RelB mutation.
437 citations
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TL;DR: Radioactive iodide was covalently bound to living cells from normal mouse spleen and a variety of lymphoid tumours by a system consisting of lactoperoxidase, hydrogen peroxide and iodide and Electron-micrographic radioautography showed that the radioactive label was associated with the outer surfaces of the cells.
Abstract: 1. Radioactive iodide was covalently bound to living cells from normal mouse spleen and a variety of lymphoid tumours by a system consisting of lactoperoxidase, hydrogen peroxide and iodide. 2. About 3×10 5 -6×10 5 molecules of [ 125 I]iodide/cell could be incorporated without affecting cell viability. 3. Electron-micrographic radioautography showed that the radioactive label was associated with the outer surfaces of the cells. 4. Radioiodinated proteins were solubilized in 9m-urea–0.2m-mercaptoethanol and analysed by gel-filtration and disc electrophoresis. 5. Comparison of distinct tumour lines by disc electrophoresis showed qualitative and quantitative differences in protein distribution patterns.
436 citations
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TL;DR: This work found that transcriptional signatures of the cDC1 and cDC2 lineages became evident at the single-cell level from the CDP stage, and identified Siglec-H and Ly6C as lineage markers that distinguished pre-DC subpopulations committed to the c DC1 lineage (SigleC-H−Ly6C− pre- DCs) or c DC2 lineage (CDC2 lineage).
Abstract: Mouse conventional dendritic cells (cDCs) can be classified into two functionally distinct lineages: the CD8α(+) (CD103(+)) cDC1 lineage, and the CD11b(+) cDC2 lineage. cDCs arise from a cascade of bone marrow (BM) DC-committed progenitor cells that include the common DC progenitors (CDPs) and pre-DCs, which exit the BM and seed peripheral tissues before differentiating locally into mature cDCs. Where and when commitment to the cDC1 or cDC2 lineage occurs remains poorly understood. Here we found that transcriptional signatures of the cDC1 and cDC2 lineages became evident at the single-cell level from the CDP stage. We also identified Siglec-H and Ly6C as lineage markers that distinguished pre-DC subpopulations committed to the cDC1 lineage (Siglec-H(-)Ly6C(-) pre-DCs) or cDC2 lineage (Siglec-H(-)Ly6C(+) pre-DCs). Our results indicate that commitment to the cDC1 or cDC2 lineage occurs in the BM and not in the periphery.
434 citations
Authors
Showing all 5041 results
Name | H-index | Papers | Citations |
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Martin White | 196 | 2038 | 232387 |
Stuart H. Orkin | 186 | 715 | 112182 |
Tien Yin Wong | 160 | 1880 | 131830 |
Mark J. Smyth | 153 | 713 | 88783 |
Anne B. Newman | 150 | 902 | 99255 |
James P. Allison | 137 | 483 | 83336 |
Scott W. Lowe | 134 | 396 | 89376 |
Rajkumar Buyya | 133 | 1066 | 95164 |
Peter Hall | 132 | 1640 | 85019 |
Ralph L. Brinster | 131 | 382 | 56455 |
Nico van Rooijen | 130 | 513 | 62623 |
David A. Hafler | 128 | 558 | 64314 |
Andreas Strasser | 128 | 509 | 66903 |
Marc Feldmann | 125 | 663 | 64916 |
Herman Waldmann | 118 | 586 | 49942 |