Showing papers in "Experimental Cell Research in 1991"

TL;DR: There is evidence for the possible involvement of both the RB and p53 proteins in the regulation of cellular senescence, and combinations of adenovirus E1A + E1B or human papillomavirus E6 + E7 genes are able to replace T-antigen functions and permit cell proliferation, whereas the individual genes do not.

TL;DR: MAC-T cells show a population doubling time of approximately 17 h and have been cultured more than 350 passages without showing any sign of senescence, and show the characteristic "cobblestone" morphology of epithelial cells when grown on plastic substratum.

TL;DR: Mitochondrial nucleoids (mt-nucleoids) of the A2780 line of cultured human cells were stained with DAPI and observed using an epifluorescence microscope and appeared to be organized compactly in mitochondria.

TL;DR: The treated cells had no swollen mitochondria and retained the ability to exclude trypan blue until the final stage of dissolution, supporting the hypothesis that the changes were apoptotic rather than necrotic.

TL;DR: Studies with human autoimmune sera identified auto-antibodies reacting with a novel antigen of 80 kDa enriched in nuclear coiled bodies and was used as a marker for this nuclear structure, subsequently named p80-coilin.

TL;DR: The inhibitory effects of the peptides used in this study produced effects on dentine resorption which were generally weaker and variable, although osteoclast cell adhesion was consistently inhibited in an Arg-Gly-Asp (RGD)-dependent manner, demonstrating that osteOClast function can be disrupted by low concentrations of the anti-vitronectin receptor antibody, 23C6.

TL;DR: Two ionophores specific for K+, valinomycin and beauvericin, induce a type of cell death very similar to apoptosis due to tumor necrosis factor, suggesting that other nonphysiological toxins might also cause apoptosis through their ability to indirectly elevate the cytoplasmic calcium concentration, without the need to invoke specific surface receptors.

TL;DR: It is suggested that periosteal-derived cells of young chicks contain mesenchymal cells which possess the potential to undergo terminal differentiation into osteogenic or chondrogenic phenotypes depending on local environmental or positional cues.

TL;DR: It is demonstrated that recombinant BMP-2B stimulates cartilage formation by chick limb bud mesoderm in vitro and is further modulated by TGF-beta isoforms.

TL;DR: HGF is a potent factor which enhances growth and migration of normal human keratinocyte under conditions of physiological Ca2+.

TL;DR: The existence of highly ordered organizational patterns of the cell nucleus appears to be beyond any doubt and it is difficult to escape the conclusion that interphase chromosome topology is important for the complex regulation of the many varied and interrelated nuclear processes.

TL;DR: Results show that changes in tissue organization can exert rapid and profound effects on the morphology and biosynthetic function of cells within the tissue.

TL;DR: The most plausible explanation seems to be that the sample degradation is the result of photochemical reactions initiated by laser excitation at 514.5 nm of as yet unidentified sensitizer molecules or complexes present in chromosomes and cells but not in purified DNA and histone protein samples.

TL;DR: Results demonstrate that ES cell lines from inbred mouse strains other than 129/Sv may be used as vectors to introduce selected mutations into the germ-line of mice.

TL;DR: The data suggest that CAM or other topoisomerase I inhibitors may be effective in some myelogenous leukemias, especially in combination with treatments synchronizing cells in S phase, as well as the sensitivity of the whole cell population to CAM.

TL;DR: Results demonstrate that inhibition of DNA fragmentation also prevents the onset of apoptosis, directly linking endonuclease activation and cell death.

TL;DR: This dermal substitute is the first one entirely composed of human collagens and supports a type IV collagen top layer which offers an excellent substrate for keratinocytes, favors their anchorage, and favors the formation of the basement membrane in vitro.

TL;DR: The results suggest that ricin, modeccin, and Pseudomonas toxin share a common pathway of intracellular transport from endosomes to the Golgi region where they are released into the cytosol.

TL;DR: It is concluded that elevation of pHi by integrins is specific and is a property shared by many members of the integrin family.

TL;DR: A cooperation between cath-D and bF GF is suggested, by which the protease could facilitate the release of bFGF from ECM and its subsequent use by breast cancer cells and/or adjacent cells involved in angiogenesis.

TL;DR: These findings directly support the recent suggestion that the CD11b/CD18 integrin is responsible for the "spontaneous oscillations" of cytosolic-free Ca2+ observed in adherent neutrophils and also explain how integrin-mediated adherence can modify the functional responsiveness of neutrophil to a subsequent agonist stimulation.

TL;DR: The blasticidin S-resistance gene (bsr), isolated from Bacillus cereus K55-S1 strain, was inserted into pSV2 plasmid vector and introduced into cultured mammalian cells by transfection and was concluded to be useful as a drug-res resistance marker for mammalian cells.

TL;DR: This cell line pair was used to compare the nonimmunosuppressive cyclosporin analog PSC 833 with several resistance-modifying agents (RMAs) for their in vitro chemosensitizing activity and for their restoration of DAU retention, and it is assumed that it acts on the P-glycoprotein, which is highly expressed on the membrane of the MDR-P388 cells, by inhibiting the function of the P

TL;DR: This study demonstrates that in addition to an acute inhibition of precursor growth, glucocorticoids exert a clear stimulation of adipose conversion, which depends mainly on the presence of insulin and the glucoc Corticoid concentration.

TL;DR: The effect of staurosporine, a potent microbial inhibitor of protein kinases, on the cell cycle of cultured fibroblast cells was investigated and showed a clear contrast to those by the other G2 arresters, trichostatin A and leptomycin B, which formed proliferative tetraploid cells after release by entering the cells into a new S phase without passage through M phase.

TL;DR: A novel lamin/chromatin binding assay was developed that quantitated the binding of soluble, radiolabeled lamins to minichromosomes assembled in Xenopus laevis oocyte nuclear extracts and defined carboxy-terminal sequence elements of LA and LII required for the observed lamin-chromatin interaction that are rich in serine, threonine, and glycine residues.

TL;DR: It is hypothesized that RARs are involved in the suppression of squamous differentiation in tracheobronchial epithelial cells and that lung fibroblasts are target cells for retinoids.

TL;DR: It is shown that a derivative of the avian spleen necrosis virus carrying the marker gene lacZ, designated CXL, has the potential to mark a wide variety of chick embryo cell types by infection in ovo and can provide a significant advantage over alternative lacZ vectors, especially in lineage marking of early stage embryos.

TL;DR: The adenosine analogue 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB) unravels the compact nucleoli to necklace-like structures when applied to living cells, implying that formerly inactive rRNA genes are recruited for transcription.

TL;DR: Using the isolated HDs for immunization, monoclonal antibodies specific for the 230- and 180-kDa polypeptides were prepared and showed that both were exclusively located in HDs, useful in studies of the molecular organization of HDs and make HD research independent from human autoimmune antibodies.