Showing papers in "Experimental Eye Research in 2003"

TL;DR: A revised interpretation of human corneal nerve architecture is presented based on recent observations obtained by in vivo confocal microscopy (IVCM), immunohistochemistry, and ultrastructural analyses of serial-sectionedhuman corneas.

TL;DR: The susceptibility of mtDNA to oxidative damage in human RPE cells, together with the age-related decrease of cellular anti-oxidant system, provides the rationale for a mitochondria-based model of AMD.

TL;DR: Activated microglia may be a missing link in understanding why initial rod cell death in the human diseases RP, L-ORD, and AMD leads to death of the cones that are critical for high acuity daytime vision.

TL;DR: The qualitative and quantitative differences in the cellular wound healing response after PRK for high and low myopia and LASIK for high myopia are likely determinants of the clinical differences in refractive outcome and some of the complications, such as regression and haze, seen after these procedures.

TL;DR: The content of melanin in RPE cells undergoes an age-related change to which photo-oxidation may contribute, raising the question of whether age- related changes in melanin reduce the photoprotective role of the pigment in aging R PE cells.

TL;DR: It is reported for the first time that the keratocyte phenotype is non-contractile and that cell quiescence is not a defining characteristic, and it is established that changes in environmental conditions modulate the ker atocyte phenotype resulting in physiologically functional differences regarding cell contractility and capacity to contract extracellular matrix.

TL;DR: Pharmacological alteration of AQP function may provide a new approach for therapy of glaucoma, corneal edema, and other diseases of the eye associated with abnormalities in IOP or tissue hydration.

TL;DR: Not only occludin, but also some claudins act as integral transmembrane proteins in the corneal epithelium.

TL;DR: Investigating whether transforming growth factor-beta2, increased in the aqueous humor of eyes with primary open angle glaucoma (POAG), can affect factors responsible for the activity of matrix metalloproteinases (MMPs) in human trabecular cell cultures found it appeared that treatment with TGF- beta2 significantly increased expression of the proform of MMP-2, whereas the active form was not detectable.

TL;DR: BQ123 antagonizes the effects of exogenously administeredET-1 on retinal blood flow in healthy subjects, compatible with the hypothesis that ET-1 exerts its vasoconstrictor effects in the retina mainly on the microvessels.

TL;DR: The air-lifting technique reduces intercellular spaces in the superficial cells and promotes the formation of the barrier function, which is useful in culturing corneal epithelial cells for use in ocular surface reconstruction.

TL;DR: The results demonstrate that the P2Y(2) receptor gene is expressed in a variety of ocular cells types and suggest that P2y( 2) receptors are associated with diverse physiological functions throughout the eye.

TL;DR: It is concluded that local antagonism of IL-1 after alkali injury can significantly decreaseCorneal inflammation and lead to enhanced corneal transparency.

TL;DR: The ability to confer neuroprotection to retinal neurones is a common feature of three ophthalmic beta-adrenoceptor antagonists (betaxolol, metipranolol and timolol), which appears to relate to their capacity to attenuate neuronal calcium and sodium influx.

TL;DR: Results show that 24-hr IOP pattern in mouse eyes is biphasic and that extended exposure to constant light disrupts this 24- Hour intraocular pressure pattern.

TL;DR: Limitations do exit, however a greater understanding of the molecular biology of eye tissues coupled with the development of low immunogenicity vectors will continue edging the way for a future use of gene therapy in the clinical setting.

TL;DR: The CP49 knockout mouse is therefore an important model to study the functional link between lens transparency, the cytoskeleton and plasma membrane organisation as revealed by detailed morphological examinations.

TL;DR: Minocycline has its greatest effect on lipid types, which result from degradation (lipase) reactions, suggesting a lipase inhibition effect and/or direct effect on ocular flora.

TL;DR: Culture of RPE with EC reduces RPE barrier properties and the reduction is, in part, mediated by soluble VEGF, which occurs in ocular pathologies such as choroidal neovascularization.

TL;DR: Evaluated corneal organ culture provides an adequate system for elucidating mechanisms of epithelial alterations in human DR corneas, and epithelial wound healing was much slower in organ-cultured DRCorneas than in normal corneAs, in complete accordance with clinical data in diabetic patients.

TL;DR: Magnetic attachment of iron-endocytosed corneal endothelial cells to Descemet's nembrane can be a method of choice for cornean endothelial decompensation.

TL;DR: Findings support a possible role for crystallins in protecting photoreceptors from lightDamage and suggest anti-alphaB-crystallin immunoreactivity was increased in rod outer segments and retinal pigment epithelium.

TL;DR: The role of corneal nerves passing between the stroma and epithelium at the sites of early degradative change observed previously are investigated, and may be facilitating the keratocyte-epithelial interactions in this disease.

TL;DR: It is proposed that the high relative contents of DHSMs provide a biochemically inert matrix in which only small amounts of PCs and SMs and their metabolites, known to promote and arrest growth, respectively, are present, to contribute to the slow multiplication and elongation of human lens cells.

TL;DR: Compared the onsets of expression of the classical visual opsins with those of the non-rod, non-cone opsins in foetal and post-natal eye tissue from mice and humans are compared to discuss the possible functions of these opsins at early stages of ocular development.

TL;DR: This study demonstrates matrix metalloproteinase expression in epithelial and fibroblast-like cells following wounding of human corneal tissue in culture where the cells remain in contact with their natural matrices.

TL;DR: It is suggested that in an intact tissue the ability of a cell to withstand a metabolic challenge will depend very much on the adequacy of the supply of glucose.

TL;DR: The MAP kinase inhibitor U0126 prevented activation of ERK1/2 in LECs and inhibited EF-directed migration, implicatingMAP kinase signaling in directing the migration of L ECs in a physiological EF.

TL;DR: Wogonin may inhibit IL-6 and IL-8 mRNA expression via the suppression of NF-kappaB binding activities in human retinal pigment epithelial cell line (ARPE-19) cells.

TL;DR: The data suggest that H(2)O is cotransported along with H(+) and lactate ions in MCT1 localized to the retinal membrane and supports the previous study performed by an invasive technique in an amphibian tissue.