A bioinformatics analysis of exosomal microRNAs released following mycobacterial infection
TLDR
This analysis demonstrated that these dysregulated miRNAs have central roles in the host metabolic and energy pathways, and suggests that the host miRNA network is perturbed by Mycobacterium to re-patterning host metabolism machinery to favor its intracellular survival.Abstract:
Background: Tuberculosis (TB) still remains a major health threat worldwide. The current TB diagnostics are suboptimal, and there is a high clinical need for identifying novel biomarkers of disease prevalence. Circulating exosomes have been currently attractive as novel biomarkers in a wide range of pathological conditions. Methods: In this study, we performed bioinformatics analysis on the downstream targets of a dysregulated microRNA (miRNA) cluster induced by Bacillus Calmette–Guerin infection of human macrophages to provide greater understanding of their potential roles in disease pathogenesis. Results: Our analysis demonstrated that these dysregulated miRNAs have central roles in the host metabolic and energy pathways. Conclusion: This suggests that the host miRNA network is perturbed by Mycobacterium to re-patterning host metabolism machinery to favor its intracellular survival. The dysregulated miRNAs can be delivered to local and distal cells by exosomes and thereby modulate their function.read more
Citations
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The emerging role of exosomal miRNAs as a diagnostic and therapeutic biomarker in Mycobacterium tuberculosis infection.
Rasoul Mirzaei,Sajad Babakhani,Parisa Ajorloo,Razieh Ahmadi,Seyed Reza Hosseini-Fard,Hossein Keyvani,Yaghoub Ahmadyousefi,Ali Teimoori,Farhad Zamani,Sajad Karampoor,Rasoul Yousefimashouf +10 more
TL;DR: In this paper, the authors explored the possible function of exosomal miRNAs as a diagnostic biomarker for TB and LTBI, and included the latest data on the pathogenic and therapeutic role of Exosomal MiRNAs in TB.
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Emerging Role of Exosomes in Diagnosis and Treatment of Infectious and Inflammatory Bowel Diseases
TL;DR: Evidence suggests that exosomes could be used as vaccines to prime the immune system to recognize and kill invading pathogens, and as therapeutic components relieving intestinal inflammation as biomarkers, vaccines and conveyers of therapeutic molecules in inflammatory bowel diseases.
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Extracellular Vesicles and Asthma—More Than Just a Co-Existence
Bilal Alashkar Alhamwe,Bilal Alashkar Alhamwe,Daniel P. Potaczek,Sarah Miethe,Fahd Alhamdan,Lukas Hintz,Arslan Magomedov,Holger Garn +7 more
TL;DR: In this article, the authors summarize the functional relevance of extracellular vesicles (EVs) in asthma, one of the most common chronic non-communicable diseases.
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Small RNA sequencing and bioinformatics analysis of RAW264.7-derived exosomes after Mycobacterium Bovis Bacillus Calmette-Guérin infection
TL;DR: In this article , the authors investigated the possible related mechanisms at the exosomal miRNA level and found that Bacillus Calmette-Guérin (BCG) infection could alter the composition of the macrophage exosome content, and the expression levels of miRNAs in exosomes derived from the cell culture media of macrophages showed significant differences between the BCG-infected and noninfected groups.
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