Journal ArticleDOI
A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry
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TLDR
A flow cytometric method for measuring the percentage of apoptotic nuclei after propidium iodide staining in hypotonic buffer is developed and shown an excellent correlation with the results obtained with both electrophoretic and colorimetric methods.About:
This article is published in Journal of Immunological Methods.The article was published on 1991-06-03. It has received 4660 citations till now. The article focuses on the topics: Propidium iodide & DNA fragmentation.read more
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The neutrophil gelatinase-associated lipocalin (NGAL), a NF-κB-regulated gene, is a survival factor for thyroid neoplastic cells
Alessio Iannetti,Francesco Pacifico,Renato Acquaviva,Alfonso Lavorgna,Elvira Crescenzi,Carlo Vascotto,Gianluca Tell,Anna Maria Salzano,Andrea Scaloni,Emilia Vuttariello,Gennaro Chiappetta,S. Formisano,Antonio Leonardi +12 more
TL;DR: The data suggest that NF-κB contributes to thyroid tumor cell survival by controlling iron uptake via NGAL, a protein involved in inflammatory and immune responses, and which is highly expressed in human thyroid carcinomas.
Journal ArticleDOI
CXCR4 and CD4 mediate a rapid CD95-independent cell death in CD4+ T cells
TL;DR: This work used human peripheral blood lymphocytes, malignant T cells, and CD4/CXCR4 transfectants, and found cell death induced by both cell surface receptors, CD4 and CX CR4, which might contribute to T cell depletion in AIDS and might have major implications for therapeutic intervention.
Journal Article
Modulation of Hsp90 function by ansamycins sensitizes breast cancer cells to chemotherapy-induced apoptosis in an RB- and schedule-dependent manner. See: E. A. Sausville, Combining cytotoxics and 17-allylamino, 17-demethoxygeldanamycin: sequence and tumor biology matters, Clin. Cancer Res., 7: 2155-2158, 2001.
TL;DR: Inhibition of Hsp90 function by 17-AAG enhances the apoptotic effects of cytotoxic agents and the sequence of drug administration and the RB status significantly influence efficacy.
Journal ArticleDOI
Sustained activation of AMP-activated protein kinase induces c-Jun N-terminal kinase activation and apoptosis in liver cells.
D. Meisse,Mark Van de Casteele,Christophe Beauloye,Isabelle Hainault,Benjamin A. Kefas,Mark H. Rider,Fabienne Foufelle,Louis Hue +7 more
TL;DR: Experiments supported the notion that prolonged AMPK activation in liver cells induces apoptosis through an activation pathway that involves JNK and caspase‐3.
Journal ArticleDOI
Increased membrane permeability of apoptotic thymocytes: A flow cytometric study
M. G. Ormerod,Xiao-Ming Sun,Roger T. Snowden,Reginald Davies,Howard O. Fearnhead,Gerald M. Cohen +5 more
TL;DR: It is shown that the efflux of fluorescein from apoptotic cells is more rapid than that from normal thymocytes, and an increase in the permeability of the plasma membrane of the apoptoticThymocytes results in the more rapid uptake of Hoechst 33342.
References
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Journal ArticleDOI
A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid
TL;DR: The present study arose from the observation that a more intense colour was sometimes produced if, instead of being heated at 1000 for 10 min., the reaction mixture was allowed to stand overnight at room temperature.
Book ChapterDOI
Cell death : the significance of apoptosis
TL;DR: It has proved feasible to categorize most if not all dying cells into one or the other of two discrete and distinctive patterns of morphological change, which have, generally, been found to occur under disparate but individually characteristic circumstances.
Book
Practical Flow Cytometry
TL;DR: Using Flow Cytometers: Applications, Extensions, and Alternatives as mentioned in this paper is an excellent overview of the history of the field of Flow Sorting, its applications, extensions, and alternatives.
Journal ArticleDOI
Monoclonal antibody-mediated tumor regression by induction of apoptosis
B. C. Trauth,C. Klas,Anke M.J. Peters,Siegfried Matzku,Peter Möller,Werner Falk,Klaus-Michael Debatin,Peter H. Krammer +7 more
TL;DR: Histological thin sections of the regressing tumor showed that anti-APO-1 was able to induce apoptosis in vivo, suggesting induction of apoptosis as a consequence of a signal mediated through cell surface molecules like APO- 1 may be a useful therapeutic approach in treatment of malignancy.
Journal ArticleDOI
Chromatin cleavage in apoptosis: association with condensed chromatin morphology and dependence on macromolecular synthesis.
TL;DR: The data confirm that the condensed chromatin which characterizes apoptosis morphologically consists of endogenously digested chromatin fragments, and provide support for the view that at least some cells enter apoptosis by a process dependent upon macromolecular synthesis.