Journal ArticleDOI
A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry
Reads0
Chats0
TLDR
A flow cytometric method for measuring the percentage of apoptotic nuclei after propidium iodide staining in hypotonic buffer is developed and shown an excellent correlation with the results obtained with both electrophoretic and colorimetric methods.About:
This article is published in Journal of Immunological Methods.The article was published on 1991-06-03. It has received 4660 citations till now. The article focuses on the topics: Propidium iodide & DNA fragmentation.read more
Citations
More filters
Journal ArticleDOI
Mutation of CD95 (Fas/Apo-1) Gene in Adult T-Cell Leukemia Cells
TL;DR: The finding that Fas-negative ATL cells were resistant to adriamycin-induced apoptosis in vitro, which is consistent with the finding that ATL in this case was resistant to chemotherapy, indicates that mutation of the Fas gene may be associated with the progression of ATL and with resistance to anticancer drugs.
Journal ArticleDOI
A Chemical and Genetic Approach Together Define the Biological Consequences of 3-Methyladenine Lesions in the Mammalian Genome
Bevin P. Engelward,James M. Allan,Andrew J. Dreslin,Jack D. Kelly,Mavis M. Wu,Barry Gold,Leona D. Samson +6 more
TL;DR: The combined use of these two experimental tools has defined the biological consequences of 3-methyladenine, a DNA lesion produced by endogenous cellular metabolites, environmental carcinogens, and chemotherapeutic alkylating agents.
Journal ArticleDOI
Alveolar macrophages that phagocytose apoptotic neutrophils produce hepatocyte growth factor during bacterial pneumonia in mice.
Kounosuke Morimoto,Hideaki Amano,Fuminari Sonoda,Motoo Baba,Masachika Senba,Hiroyuki Yoshimine,Hidefumi Yamamoto,Tsuyoshi,Kazunori Oishi,Tsuyoshi Nagatake +9 more
TL;DR: Results strongly indicate a novel mechanism of HGF production by AMs, which are phagocytosing apoptotic neutrophils, and the pivotal role of AMs in the healing and repair of damaged pulmonary epithelium through the production of H GF.
Journal ArticleDOI
Cellular disposition of sulphamethoxazole and its metabolites: implications for hypersensitivity.
Dean J. Naisbitt,Sally J Hough,Helen J. Gill,Munir Pirmohamed,Neil R. Kitteringham,B. Kevin Park +5 more
TL;DR: The results suggest that the balance between oxidation of SMX to its toxic metabolites and their reduction is an important protective cellular mechanism.
Journal ArticleDOI
hTERT: a novel endogenous inhibitor of the mitochondrial cell death pathway.
Christophe Massard,Zermati Y,Anne-Laure Pauleau,Nathanael Larochette,Didier Métivier,Laure Sabatier,Guido Kroemer,J-C. Soria +7 more
TL;DR: In this article, the authors show that hTERT depletion has no adverse effects on the viability or proliferation of cervical and colon carcinoma cell lines, as evaluated within 72 h after transfection with hTERTs-specific small interfering RNAs (siRNAs).
References
More filters
Journal ArticleDOI
A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid
TL;DR: The present study arose from the observation that a more intense colour was sometimes produced if, instead of being heated at 1000 for 10 min., the reaction mixture was allowed to stand overnight at room temperature.
Book ChapterDOI
Cell death : the significance of apoptosis
TL;DR: It has proved feasible to categorize most if not all dying cells into one or the other of two discrete and distinctive patterns of morphological change, which have, generally, been found to occur under disparate but individually characteristic circumstances.
Book
Practical Flow Cytometry
TL;DR: Using Flow Cytometers: Applications, Extensions, and Alternatives as mentioned in this paper is an excellent overview of the history of the field of Flow Sorting, its applications, extensions, and alternatives.
Journal ArticleDOI
Monoclonal antibody-mediated tumor regression by induction of apoptosis
B. C. Trauth,C. Klas,Anke M.J. Peters,Siegfried Matzku,Peter Möller,Werner Falk,Klaus-Michael Debatin,Peter H. Krammer +7 more
TL;DR: Histological thin sections of the regressing tumor showed that anti-APO-1 was able to induce apoptosis in vivo, suggesting induction of apoptosis as a consequence of a signal mediated through cell surface molecules like APO- 1 may be a useful therapeutic approach in treatment of malignancy.
Journal ArticleDOI
Chromatin cleavage in apoptosis: association with condensed chromatin morphology and dependence on macromolecular synthesis.
TL;DR: The data confirm that the condensed chromatin which characterizes apoptosis morphologically consists of endogenously digested chromatin fragments, and provide support for the view that at least some cells enter apoptosis by a process dependent upon macromolecular synthesis.