Journal ArticleDOI
A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry
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TLDR
A flow cytometric method for measuring the percentage of apoptotic nuclei after propidium iodide staining in hypotonic buffer is developed and shown an excellent correlation with the results obtained with both electrophoretic and colorimetric methods.About:
This article is published in Journal of Immunological Methods.The article was published on 1991-06-03. It has received 4660 citations till now. The article focuses on the topics: Propidium iodide & DNA fragmentation.read more
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Journal ArticleDOI
Acetylsalicylic acid (ASA) blocks influenza virus propagation via its NF-kappa B-inhibiting activity
Igor Mazur,Walter J. Wurzer,Christina Ehrhardt,Stephan Pleschka,Pilaipan Puthavathana,Tobias Silberzahn,Thorsten Wolff,Oliver Planz,Stephan Ludwig +8 more
TL;DR: In this article, the authors showed that specific targeting of a cellular factor is a suitable approach for anti-influenza virus intervention and showed that the drug efficiently blocks influenza virus replication in vitro and in vivo.
Journal ArticleDOI
S100A8/A9 induces autophagy and apoptosis via ROS-mediated cross-talk between mitochondria and lysosomes that involves BNIP3.
Saeid Ghavami,Mehdi Eshragi,Sudharsana R. Ande,Walter J. Chazin,Thomas Klonisch,Andrew J. Halayko,Karol D. McNeill,Mohammad Hashemi,Claus Kerkhoff,Marek Los +9 more
TL;DR: The data indicate that S100A8/A9-promoted cell death occurs through the cross-talk of mitochondria and lysosomes via ROS and the process involves BNIP3.
Journal ArticleDOI
Adenine nucleotide translocator mediates the mitochondrial membrane permeabilization induced by lonidamine, arsenite and CD437
Anne-Sophie Belzacq,Chahrazed El Hamel,Helena L. A. Vieira,Isabel Cohen,Delphine Haouzi,Didier Métivier,Philippe Marchetti,Catherine Brenner,Catherine Brenner,Guido Kroemer +9 more
TL;DR: It is indicated that ANT is a target of lonidamine, arsenite, and CD437 and an unexpected heterogeneity in the mode of action of these three compounds is unraveled.
Journal ArticleDOI
Chloromethyl-X-Rosamine is an aldehyde-fixable potential-sensitive fluorochrome for the detection of early apoptosis.
Antonio Macho,Didier Decaudin,Maria Castedo,Tamara Hirsch,Santos A. Susin,Naoufal Zamzami,Guido Kroemer +6 more
TL;DR: Chloromethyl-X-rosamine (CMXRos) is shown to be a viable alternative to other delta psi m-sensitive probes, and that it allows for formaldehyde fixation of cells before analysis, and provides several methodological advantages over other, nonfixable fluorochromes used forDelta psi m determination.
Journal ArticleDOI
Neisserial porin (PorB) causes rapid calcium influx in target cells and induces apoptosis by the activation of cysteine proteases
TL;DR: This work reports on the induction of programmed cell death after prolonged infection of epithelial cells with pathogenic Neisseria species, and proposes an underlying mechanism that includes translocation of the porin, a transient increase in cytosolic Ca2+ and subsequent activation of the Ca2+.
References
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Journal ArticleDOI
A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid
TL;DR: The present study arose from the observation that a more intense colour was sometimes produced if, instead of being heated at 1000 for 10 min., the reaction mixture was allowed to stand overnight at room temperature.
Book ChapterDOI
Cell death : the significance of apoptosis
TL;DR: It has proved feasible to categorize most if not all dying cells into one or the other of two discrete and distinctive patterns of morphological change, which have, generally, been found to occur under disparate but individually characteristic circumstances.
Book
Practical Flow Cytometry
TL;DR: Using Flow Cytometers: Applications, Extensions, and Alternatives as mentioned in this paper is an excellent overview of the history of the field of Flow Sorting, its applications, extensions, and alternatives.
Journal ArticleDOI
Monoclonal antibody-mediated tumor regression by induction of apoptosis
B. C. Trauth,C. Klas,Anke M.J. Peters,Siegfried Matzku,Peter Möller,Werner Falk,Klaus-Michael Debatin,Peter H. Krammer +7 more
TL;DR: Histological thin sections of the regressing tumor showed that anti-APO-1 was able to induce apoptosis in vivo, suggesting induction of apoptosis as a consequence of a signal mediated through cell surface molecules like APO- 1 may be a useful therapeutic approach in treatment of malignancy.
Journal ArticleDOI
Chromatin cleavage in apoptosis: association with condensed chromatin morphology and dependence on macromolecular synthesis.
TL;DR: The data confirm that the condensed chromatin which characterizes apoptosis morphologically consists of endogenously digested chromatin fragments, and provide support for the view that at least some cells enter apoptosis by a process dependent upon macromolecular synthesis.