Journal ArticleDOI
A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry
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TLDR
A flow cytometric method for measuring the percentage of apoptotic nuclei after propidium iodide staining in hypotonic buffer is developed and shown an excellent correlation with the results obtained with both electrophoretic and colorimetric methods.About:
This article is published in Journal of Immunological Methods.The article was published on 1991-06-03. It has received 4660 citations till now. The article focuses on the topics: Propidium iodide & DNA fragmentation.read more
Citations
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Journal ArticleDOI
Decreased susceptibility to oxidative stress-induced apoptosis of peripheral blood mononuclear cells from healthy elderly and centenarians.
Daniela Monti,Stefano Salvioli,Miriam Capri,Walter Malorni,Elisabetta Straface,Andrea Cossarizza,Barbara Botti,Mauro Piacentini,Giovannella Baggio,Giovannella Baggio,Cristiana Barbi,Silvana Valensin,Massimiliano Bonafè,Claudio Franceschi +13 more
TL;DR: Physiological aging is characterised by a decreased tendency to undergo apoptosis, a phenomenon likely resulting from adaptation to lifelong exposure to damaging agents, such as reactive oxygen species, and may contribute to one of the major phenomena of immunosenescence, i.e. the progressive accumulation of memory/effector T cells.
Book ChapterDOI
Chapter 24 Difficulties and pitfalls in analysis of apoptosis
TL;DR: This chapter discusses the most common errors in measurement of apoptosis as well as the frequent mistakes in the analysis and interpretation of the data.
Journal ArticleDOI
Primary DNA damage in chrome-plating workers
Angela Gambelunghe,R. Piccinini,M. Ambrogi,Milena Villarini,Massimo Moretti,Cristina Marchetti,Giuseppe Abbritti,Giacomo Muzi +7 more
TL;DR: The study confirms that measurements of chromium in erythrocytes and lymphocytes may provide useful information about recent and past exposure to hexavalent chromium at the workplace and suggests this test is valid for the biological monitoring of workers exposed to genotoxic compounds such as chromium (VI).
Journal ArticleDOI
Interleukin-13 inhibits interleukin-2-induced proliferation and protects chronic lymphocytic leukemia B cells from in vitro apoptosis
Nadia Chaouchi,Christine Wallon,Cέcile Goujard,Gέrard Tertian,Annick Rudent,Daniel Caput,Pascal Ferrera,Adrian Minty,Aimέ Vazquez,Jean-François Delfraissy +9 more
TL;DR: The present observations show that IL-13 may exhibit a negative regulatory effect on neoplasic B cells in contrast with that observed in normal B cells, and suggest thatIL-13 could be an important factor in the pathogenesis of CLL by preventing the death of monoclonal B cells.
Journal ArticleDOI
HLA class II-mediated death is induced via Fas/Fas ligand interactions in human splenic B lymphocytes.
Jean-Philip Truman,Jean-Philip Truman,Jean-Philip Truman,Christine Choqueux,Christine Choqueux,Christine Choqueux,Jürg Tschopp,Jürg Tschopp,Jürg Tschopp,Jocelyn Vedrenne,Jocelyn Vedrenne,Jocelyn Vedrenne,Françoise Le Deist,Françoise Le Deist,Françoise Le Deist,Dominique Charron,Dominique Charron,Dominique Charron,Nuala Mooney,Nuala Mooney,Nuala Mooney +20 more
TL;DR: Data demonstrate FasL expression on activated human B lymphocytes and support the idea that antigen presentation could contribute to the regulation of lymphocyte populations via Fas and FasL interactions, as well as confirming the crucial role of Fas/FasL interactions.
References
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Journal ArticleDOI
A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid
TL;DR: The present study arose from the observation that a more intense colour was sometimes produced if, instead of being heated at 1000 for 10 min., the reaction mixture was allowed to stand overnight at room temperature.
Book ChapterDOI
Cell death : the significance of apoptosis
TL;DR: It has proved feasible to categorize most if not all dying cells into one or the other of two discrete and distinctive patterns of morphological change, which have, generally, been found to occur under disparate but individually characteristic circumstances.
Book
Practical Flow Cytometry
TL;DR: Using Flow Cytometers: Applications, Extensions, and Alternatives as mentioned in this paper is an excellent overview of the history of the field of Flow Sorting, its applications, extensions, and alternatives.
Journal ArticleDOI
Monoclonal antibody-mediated tumor regression by induction of apoptosis
B. C. Trauth,C. Klas,Anke M.J. Peters,Siegfried Matzku,Peter Möller,Werner Falk,Klaus-Michael Debatin,Peter H. Krammer +7 more
TL;DR: Histological thin sections of the regressing tumor showed that anti-APO-1 was able to induce apoptosis in vivo, suggesting induction of apoptosis as a consequence of a signal mediated through cell surface molecules like APO- 1 may be a useful therapeutic approach in treatment of malignancy.
Journal ArticleDOI
Chromatin cleavage in apoptosis: association with condensed chromatin morphology and dependence on macromolecular synthesis.
TL;DR: The data confirm that the condensed chromatin which characterizes apoptosis morphologically consists of endogenously digested chromatin fragments, and provide support for the view that at least some cells enter apoptosis by a process dependent upon macromolecular synthesis.