A role for the GCC-box in jasmonate-mediated activation of the PDF1.2 gene of arabidopsis
TLDR
The PDF1.2 gene of Arabidopsis encoding a plant defensin is commonly used as a marker for characterization of the jasmonate-dependent defense responses and ethylene response factors also appear to play important roles in regulating jasMonate-responsive gene expression, possibly via interaction with the GCC-box.Abstract:
The PDF1.2 gene of Arabidopsis encoding a plant defensin is commonly used as a marker for characterization of the jasmonate-dependent defense responses. Here, using PDF1.2 promoter-deletion lines linked to the beta-glucoronidase-reporter gene, we examined putative promoter elements associated with jasmonate-responsive expression of this gene. Using stably transformed plants, we first characterized the extended promoter region that positively regulates basal expression from the PDF1.2 promoter. Second, using promoter deletion constructs including one from which the GCC-box region was deleted, we observed a substantially lower response to jasmonate than lines carrying this motif. In addition, point mutations introduced into the core GCC-box sequence substantially reduced jasmonate responsiveness, whereas addition of a 20-nucleotide-long promoter element carrying the core GCC-box and flanking nucleotides provided jasmonate responsiveness to a 35S minimal promoter. Taken together, these results indicated that the GCC-box plays a key role in conferring jasmonate responsiveness to the PDF1.2 promoter. However, deletion or specific mutations introduced into the core GCC-box did not completely abolish the jasmonate responsiveness of the promoter, suggesting that the other promoter elements lying downstream from the GCC-box region may also contribute to jasmonate responsiveness. In other experiments, we identified a jasmonate- and pathogen-responsive ethylene response factor transcription factor, AtERF2, which when overexpressed in transgenic Arabidopsis plants activated transcription from the PDF1.2, Thi2.1, and PR4 (basic chitinase) genes, all of which contain a GCC-box sequence in their promoters. Our results suggest that in addition to their roles in regulating ethylene-mediated gene expression, ethylene response factors also appear to play important roles in regulating jasmonate-responsive gene expression, possibly via interaction with the GCC-box.read more
Citations
More filters
Journal ArticleDOI
Elicitor signal transduction leading to production of plant secondary metabolites.
TL;DR: Progress made on several aspects of elicitor signal transduction leading to production of plant secondary metabolites are summarized, including the integration of multiple signaling pathways into or by transcription factors, as well as the linkage of the above signal components in eliciting network through protein phosphorylation and dephosphorylation.
Journal ArticleDOI
JASMONATE-INSENSITIVE1 Encodes a MYC Transcription Factor Essential to Discriminate between Different Jasmonate-Regulated Defense Responses in Arabidopsis
TL;DR: The identification of several new loci involved in JA signaling and the characterization and positional cloning of one of them, JASMONATE-INSENSITIVE1 (JAI1/JIN1), a nuclear-localized basic helix-loop-helix-leucine zipper transcription factor whose expression is rapidly upregulated by JA, are described.
Journal ArticleDOI
Plant hormone-mediated regulation of stress responses.
TL;DR: The intricate web of crosstalk among the often redundant multitudes of signaling intermediates is just beginning to be understood and future research employing genome-scale systems biology approaches to solve problems of such magnitude will undoubtedly lead to better understanding of plant development.
Journal ArticleDOI
Antagonistic Interaction between Abscisic Acid and Jasmonate-Ethylene Signaling Pathways Modulates Defense Gene Expression and Disease Resistance in Arabidopsis
Jonathan P. Anderson,Jonathan P. Anderson,Ellet Badruzsaufari,Ellet Badruzsaufari,Peer M. Schenk,John M. Manners,John M. Manners,Olivia J. Desmond,Olivia J. Desmond,Christina Ehlert,Donald J. Maclean,Donald J. Maclean,Paul R. Ebert,Paul R. Ebert,Kemal Kazan,Kemal Kazan +15 more
TL;DR: The results indicate that the antagonistic interactions between multiple components of ABA and the JA-ethylene signaling pathways modulate defense and stress responsive gene expression in response to biotic and abiotic stresses.
Journal ArticleDOI
Functional analysis of a NAC-type transcription factor OsNAC6 involved in abiotic and biotic stress-responsive gene expression in rice
Kazuo Nakashima,Lam-Son Phan Tran,Dong Van Nguyen,Miki Fujita,Kyonoshin Maruyama,Daisuke Todaka,Yusuke Ito,Nagao Hayashi,Kazuo Shinozaki,Kazuko Yamaguchi-Shinozaki +9 more
TL;DR: In this article, a transactivation assay using a yeast system demonstrated that OsNAC6 functions as a transcriptional activator, and transient localization studies with OsAC6-sGFP fusion protein revealed its nuclear localization.
References
More filters
Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana
Steven J. Clough,Andrew F. Bent +1 more
TL;DR: The modified method should facilitate high-throughput transformation of Arabidopsis for efforts such as T-DNA gene tagging, positional cloning, or attempts at targeted gene replacement.
Journal ArticleDOI
Assaying chimeric genes in plants: The GUS gene fusion system
TL;DR: Gene fusions can be defined its DNA constructions that result in the coding sequences from one gene (r@o,ter) being transcribed and/or translated under the direction of the controlling sequences of another gene (cmltrr).
Journal ArticleDOI
DNA-binding specificity of the ERF/AP2 domain of Arabidopsis DREBs, transcription factors involved in dehydration- and cold-inducible gene expression
Yoh Sakuma,Qiang Liu,Joseph G. Dubouzet,Hiroshi Abe,Kazuo Shinozaki,Kazuko Yamaguchi-Shinozaki +5 more
TL;DR: Gel mobility shift assay using mutant DREB proteins showed that the two amino acids, valine and glutamic acid conserved in the ERF/AP2 domains, especially valine, have important roles in DNA-binding specificity.
Journal ArticleDOI
pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation.
TL;DR: The pGreen plasmid system allows any arrangement of selectable marker and reporter gene at the right and left T-DNA borders without compromising the choice of restriction sites for cloning, since the pGreen cloning sites are based on the well-known pBluescript general vector plasmids.