Journal ArticleDOI
Adhesion forces between individual ligand-receptor pairs.
TLDR
Under conditions that allowed only a limited number of molecular pairs to interact, the force required to separate tip and bead was found to be quantized in integer multiples of 160 +/- 20 piconewtons for biotin and 85 +/- 15 piconewstons for iminobiotin.Abstract:
The adhesion force between the tip of an atomic force microscope cantilever derivatized with avidin and agarose beads functionalized with biotin, desthiobiotin, or iminobiotin was measured Under conditions that allowed only a limited number of molecular pairs to interact, the force required to separate tip and bead was found to be quantized in integer multiples of 160 +/- 20 piconewtons for biotin and 85 +/- 15 piconewtons for iminobiotin The measured force quanta are interpreted as the unbinding forces of individual molecular pairsread more
Citations
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Synthesis and surface engineering of iron oxide nanoparticles for biomedical applications
Ajay Kumar Gupta,Mona Gupta +1 more
TL;DR: This review discusses the synthetic chemistry, fluid stabilization and surface modification of superparamagnetic iron oxide nanoparticles, as well as their use for above biomedical applications.
Journal ArticleDOI
Force measurements with the atomic force microscope: Technique, interpretation and applications
TL;DR: The atomic force microscope (AFM) is not only used to image the topography of solid surfaces at high resolution but also to measure force-versus-distance curves as discussed by the authors, which provide valuable information on local material properties such as elasticity, hardness, Hamaker constant, adhesion and surface charge densities.
Journal ArticleDOI
Dynamic strength of molecular adhesion bonds.
Evan Evans,Ken Ritchie +1 more
TL;DR: How Brownian dynamics can help bridge the gap between molecular dynamics and probe tests is described, which shows that bond strength progresses through three dynamic regimes of loading rate.
Journal ArticleDOI
Single-molecule force spectroscopy: optical tweezers, magnetic tweezers and atomic force microscopy
Keir C. Neuman,Attila Nagy +1 more
TL;DR: These techniques are described and illustrated with examples highlighting current capabilities and limitations of single-molecule force spectroscopy.
Journal ArticleDOI
Dynamic atomic force microscopy methods
Ricardo Garcia,Rubén Pérez +1 more
TL;DR: In this paper, the authors review the fundamentals, applications and future tendencies of dynamic atomic force microscopy (AFM) methods and present a detailed quantitative comparison between theoretical simulations and experiment.
References
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Book
Intermolecular and surface forces
TL;DR: The forces between atoms and molecules are discussed in detail in this article, including the van der Waals forces between surfaces, and the forces between particles and surfaces, as well as their interactions with other forces.
Journal ArticleDOI
Atomic force microscope
TL;DR: The atomic force microscope as mentioned in this paper is a combination of the principles of the scanning tunneling microscope and the stylus profilometer, which was proposed as a method to measure forces as small as 10-18 N. As one application for this concept, they introduce a new type of microscope capable of investigating surfaces of insulators on an atomic scale.
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Mechanotransduction across the cell surface and through the cytoskeleton
TL;DR: The results suggest that integrins act as mechanoreceptors and transmit mechanical signals to the cytoskeleton, which may be mediated simultaneously at multiple locations inside the cell through force-induced rearrangements within a tensionally integrated cytos skeleton.
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Optical trapping and manipulation of single cells using infrared laser beams
TL;DR: The use of infrared (IR) light is used to make much improved laser traps with significantly less optical damage to a variety of living cells, and new manipulative techniques using IR light are capable of producing large forces under damage-free conditions and improve the prospects for wider use of optical manipulation techniques in microbiology.
Journal ArticleDOI
Direct observation of kinesin stepping by optical trapping interferometry
TL;DR: It is found that kinesin moves with 8-nm steps, similar to biological motors that move with regular steps.