Altered high-density lipoprotein composition and functions during severe COVID-19.
Floran Begue,Sébastien Tanaka,Zarouki Mouktadi,Philippe Rondeau,Bryan Veeren,Nicolas Diotel,Alexy Tran-Dinh,Tiphaine Robert,Erick Vélia,Patrick Mavingui,Marie Lagrange-Xelot,Philippe Montravers,Philippe Montravers,David Couret,Olivier Meilhac +14 more
TLDR
In this article, HDL-C was found to be less protective in endothelial cells stiumalted by TNFα (permeability, VE-cadherin disorganization and apoptosis).Abstract:
Coronavirus disease 2019 (COVID-19) pandemic is affecting millions of patients worldwide. The consequences of initial exposure to SARS-CoV-2 go beyond pulmonary damage, with a particular impact on lipid metabolism. Decreased levels in HDL-C were reported in COVID-19 patients. Since HDL particles display antioxidant, anti-inflammatory and potential anti-infectious properties, we aimed at characterizing HDL proteome and functionality during COVID-19 relative to healthy subjects. HDLs were isolated from plasma of 8 severe COVID-19 patients sampled at admission to intensive care unit (Day 1, D1) at D3 and D7, and from 16 sex- and age-matched healthy subjects. Proteomic analysis was performed by LC-MS/MS. The relative amounts of proteins identified in HDLs were compared between COVID-19 and controls. apolipoprotein A-I and paraoxonase 1 were confirmed by Western-blot analysis to be less abundant in COVID-19 versus controls, whereas serum amyloid A and alpha-1 antitrypsin were higher. HDLs from patients were less protective in endothelial cells stiumalted by TNFα (permeability, VE-cadherin disorganization and apoptosis). In these conditions, HDL inhibition of apoptosis was blunted in COVID-19 relative to controls. In conclusion, we show major changes in HDL proteome and decreased functionality in severe COVID-19 patients.read more
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Incomplete Systemic Recovery and Metabolic Phenoreversion in Post-Acute-Phase Nonhospitalized COVID-19 Patients: Implications for Assessment of Post-Acute COVID-19 Syndrome.
Elaine Holmes,Elaine Holmes,Julien Wist,Julien Wist,Reika Masuda,Samantha Lodge,Philipp Nitschke,Torben Kimhofer,Ruey Leng Loo,Sofina Begum,Berin A. Boughton,Rongchang Yang,Aude-Claire Morillon,Sung Tong Chin,Drew Hall,Monique Ryan,Sze-How Bong,Dale W. Edgar,Dale W. Edgar,John C. Lindon,Toby Richards,Bu B. Yeap,Sven Pettersson,Sven Pettersson,Manfred Spraul,Hartmut Schaefer,Nathan G. Lawler,Nicola Gray,Luke Whiley,Jeremy K. Nicholson,Jeremy K. Nicholson +30 more
TL;DR: In this paper, a multivariate metabotyping approach was used to assess the functional recovery of non-hospitalized COVID-19 patients and the possible biochemical sequelae of "Post-Acute CoV-19 Syndrome", colloquially known as long-COVID.
Journal ArticleDOI
The Endothelium Is Both a Target and a Barrier of HDL's Protective Functions.
TL;DR: In this article, a detailed understanding of the structure-function relationships underlying the multiple interactions of HDL with endothelial cells is expected to elucidate new targets and strategies for the treatment or prevention of various diseases.
Journal ArticleDOI
The HDL Proteome Watch: Compilation of studies leads to new insights on HDL function
TL;DR: The HDL Proteome Watch Database as discussed by the authors is a collection of proteins associated with HDL proteins from different laboratories across the world, including the US, Canada, Australia, and New Zealand.
Journal ArticleDOI
The HDL Proteome Watch: Compilation of studies leads to new insights on HDL function.
TL;DR: The HDL Proteome Watch Database as discussed by the authors is a collection of proteins associated with HDL, which is used to track proteomics studies from different laboratories across the world, and can offer interesting new insights into HDL function, such as immunity, inflammation, cell adhesion, hemostasis and protease regulation.
Journal ArticleDOI
Characterization by Quantitative Serum Proteomics of Immune-Related Prognostic Biomarkers for COVID-19 Symptomatology.
Margarita Villar,Margarita Villar,José Miguel Urra,Francisco J. Rodríguez-del-Río,Sara Artigas-Jerónimo,Natalia Jiménez-Collados,Elisa Ferreras-Colino,Marinela Contreras,Isabel G. Fernández de Mera,Agustín Estrada-Peña,Christian Gortázar,José de la Fuente,José de la Fuente +12 more
TL;DR: In this article, the authors used a quantitative proteomics together with multiple data analysis algorithms to characterize serum protein profiles in five cohorts from healthy to SARS-CoV-2-infected recovered (hospital discharge), nonsevere (hospitalized), and severe [at the intensive care unit (ICU)] cases with increasing systemic inflammation in comparison with healthy individuals sampled prior to the COVID-19 pandemic.
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Shotgun proteomics implicates protease inhibition and complement activation in the antiinflammatory properties of HDL
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TL;DR: The observations suggest that HDL plays previously unsuspected roles in regulating the complement system and protecting tissue from proteolysis and that the protein cargo of HDL contributes to its antiinflammatory and antiatherogenic properties.
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