An Atypical Kinase under Balancing Selection Confers Broad-Spectrum Disease Resistance in Arabidopsis
Carine Huard-Chauveau,Laure Perchepied,Marilyne Debieu,Marilyne Debieu,Susana Rivas,Susana Rivas,Thomas Kroj,Thomas Kroj,Ilona Kars,Ilona Kars,Joy Bergelson,Fabrice Roux,Dominique Roby,Dominique Roby +13 more
TLDR
The identification, map-based cloning and functional validation of QRX3 (RKS1, Resistance related KinaSe 1), conferring broad-spectrum resistance to Xanthomonas campestris (Xc), a devastating worldwide bacterial vascular pathogen of crucifers is reported.Abstract:
The failure of gene-for-gene resistance traits to provide durable and broad-spectrum resistance in an agricultural context has led to the search for genes underlying quantitative resistance in plants. Such genes have been identified in only a few cases, all for fungal or nematode resistance, and encode diverse molecular functions. However, an understanding of the molecular mechanisms of quantitative resistance variation to other enemies and the associated evolutionary forces shaping this variation remain largely unknown. We report the identification, map-based cloning and functional validation of QRX3 (RKS1, Resistance related KinaSe 1), conferring broad-spectrum resistance to Xanthomonas campestris (Xc), a devastating worldwide bacterial vascular pathogen of crucifers. RKS1 encodes an atypical kinase that mediates a quantitative resistance mechanism in plants by restricting bacterial spread from the infection site. Nested Genome-Wide Association mapping revealed a major locus corresponding to an allelic series at RKS1 at the species level. An association between variation in resistance and RKS1 transcription was found using various transgenic lines as well as in natural accessions, suggesting that regulation of RKS1 expression is a major component of quantitative resistance to Xc. The co-existence of long lived RKS1 haplotypes in A. thaliana is shared with a variety of genes involved in pathogen recognition, suggesting common selective pressures. The identification of RKS1 constitutes a starting point for deciphering the mechanisms underlying broad spectrum quantitative disease resistance that is effective against a devastating and vascular crop pathogen. Because putative RKS1 orthologous have been found in other Brassica species, RKS1 provides an exciting opportunity for plant breeders to improve resistance to black rot in crops.read more
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The Decoy Substrate of a Pathogen Effector and a Pseudokinase Specify Pathogen-Induced Modified-Self Recognition and Immunity in Plants
Guoxun Wang,Brice Roux,Feng Feng,Endrick Guy,Endrick Guy,Lin Li,Nannan Li,Xiaojuan Zhang,Martine Lautier,Marie-Françoise Jardinaud,Matthieu Chabannes,Matthieu Chabannes,Matthieu Arlat,Matthieu Arlat,Matthieu Arlat,She Chen,Chaozu He,Laurent D. Noël,Laurent D. Noël,Jianmin Zhou +19 more
TL;DR: It is shown that PBL2, a paralog of BIK1, is similarly uridylylated by AvrAC, which acts as a decoy and enables Avrac detection, and illustrates how decoy substrates and pseudokinases can specify and expand the capacity of the plant immune system.
Journal ArticleDOI
Quantitative Resistance to Plant Pathogens in Pyramiding Strategies for Durable Crop Protection.
Marie-Laure Pilet-Nayel,Benoît Moury,Valérie Caffier,Josselin Montarry,Marie-Claire Kerlan,Sylvain Fournet,Charles-Eric Durel,Régine Delourme +7 more
TL;DR: This review summarizes current knowledge on the genetic inheritance, molecular basis, and durability of quantitative resistance and discusses how strategies that combine major R genes and QTL in crops can maintain the effectiveness of plant resistance to pathogens.
Journal ArticleDOI
Quantitative Resistance: More Than Just Perception of a Pathogen
TL;DR: Previous work on qualitative resistance to focus on the mechanisms of quantitative resistance, such as the link between perception of microbe-associated molecular patterns and growth, has shown that the mechanisms underlying these defense outputs are also highly polygenic.
Journal ArticleDOI
Resistance to phytopathogens e tutti quanti: placing plant quantitative disease resistance on the map
Fabrice Roux,Fabrice Roux,Derry Voisin,Derry Voisin,Thomas Badet,Thomas Badet,Claudine Balagué,Claudine Balagué,Xavier Barlet,Xavier Barlet,Carine Huard-Chauveau,Carine Huard-Chauveau,Dominique Roby,Dominique Roby,Sylvain Raffaele,Sylvain Raffaele +15 more
TL;DR: The molecular architecture of QDR can be hypothesized as an intricate network integrating multiple response pathways to several pathogen molecular determinants and environmental cues, as revealed by crossing host parents with contrasted phenotypes.
Journal ArticleDOI
Of guards, decoys, baits and traps: pathogen perception in plants by type III effector sensors.
TL;DR: A characteristic overview of plant T3E sensors is provided and it is discussed how these sensors convey the presence of T3Es to NLR proteins to activate ETI.
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