An improved Escherichia coli donor strain for diparental mating
Sabrina Thoma,Max Schobert +1 more
TLDR
This improved method uses a new donor strain, E. coli ST18, a hemA deletion mutant mutant defective in tetrapyrrole biosynthesis, which means that counterselection of the Escherichia coli donor strain is not required.Abstract:
We present a new method for diparental mating with the outstanding advantage that counterselection of the Escherichia coli donor strain is not required. This improved method uses a new donor strain, E. coli ST18, a hemA deletion mutant defective in tetrapyrrole biosynthesis. The hemA mutation can be complemented by addition of 5-aminolevulinic acid. Therefore, counterselection is carried out only using standard media and growth conditions optimal for the recipient strain. Consequently, recipient strains are isolated in a significantly shorter period.read more
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Precision-engineering the Pseudomonas aeruginosa genome with two-step allelic exchange
Laura R. Hmelo,Bradley R. Borlee,Henrik Almblad,Michelle E. Love,Trevor E. Randall,Boo Shan Tseng,Chuyang Lin,Yasuhiko Irie,Kelly M. Storek,Jaeun Yang,Richard Siehnel,P. Lynne Howell,Pradeep K. Singh,Tim Tolker-Nielsen,Matthew R. Parsek,Herbert P. Schweizer,Joe J. Harrison +16 more
TL;DR: This protocol describes the use of this technique to make gene knockouts and knock-ins, as well as single-nucleotide insertions, deletions and substitutions, in Pseudomonas aeruginosa.
Journal ArticleDOI
Growth of E. coli on formate and methanol via the reductive glycine pathway.
Seohyoung Kim,Steffen N. Lindner,Selçuk Aslan,Oren Yishai,Sebastian Wenk,Karin Schann,Arren Bar-Even +6 more
TL;DR: The central carbon metabolism of Escherichia coli is redesigned with the reductive glycine pathway to enable growth on the one-carbon compounds formate and CO2, and the addition of methanol dehydrogenase further enables growth on meethanol andCO2.
Journal ArticleDOI
Quorum Sensing Controls Adaptive Immunity through the Regulation of Multiple CRISPR-Cas Systems.
Adrian G. Patterson,Simon A. Jackson,Corinda Taylor,Gary B. Evans,George P. C. Salmond,Rita Przybilski,Raymond H.J. Staals,Peter C. Fineran +7 more
TL;DR: It is proposed that bacteria can use chemical communication to modulate the balance between community-level defense requirements in high cell density populations and host fitness costs of basal CRISPR-Cas activity.
Journal ArticleDOI
The antimicrobial volatile power of the rhizospheric isolate Pseudomonas donghuensis P482.
TL;DR: It is demonstrated that VOCs emitted by P. donghuensis P482 have strong antifungal and antioomycete, but not antibacterial activity, as the gacA mutant entirely lost the ability to inhibit via volatiles the growth of tested plant pathogens.
Journal ArticleDOI
CpxR Activates MexAB-OprM Efflux Pump Expression and Enhances Antibiotic Resistance in Both Laboratory and Clinical nalB-Type Isolates of Pseudomonas aeruginosa.
TL;DR: It is demonstrated that CpxR, previously identified as a regulator of the cell envelope stress response in Escherichia coli, is directly involved in activation of expression of RND efflux pump MexAB-OprM in P. aeruginosa.
References
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Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
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One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
TL;DR: A simple and highly efficient method to disrupt chromosomal genes in Escherichia coli in which PCR primers provide the homology to the targeted gene(s), which should be widely useful, especially in genome analysis of E. coli and other bacteria.
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A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram negative bacteria
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A short course in bacterial genetics
TL;DR: This second edition of Molecular Cloning reflects more comprehensive coverage of topics previously included in the first edition as well as the addition of new chapters, such as those on oligonucleotide probes and mutagenesis, in vitro amplification by the polymerase chain reaction, expression of cloned genes in Escherichia coli and mammalian cells, and analysis of proteins ex pressed from cloning genes.