Journal ArticleDOI
Automatic scoring of dicentric chromosomes as a tool in large scale radiation accidents
Horst Romm,Elizabeth A. Ainsbury,Stephen Barnard,Leonardo Barrios,Joan Francesc Barquinero,Christina Beinke,M Deperas,Eric Gregoire,Koivistoinen A,Carita Lindholm,Jayne Moquet,Ursula Oestreicher,Roser Puig,Kai Rothkamm,Sylwester Sommer,Hubert Thierens,Veerle Vandersickel,Anne Vral,Andrzej Wojcik +18 more
TLDR
Semi-automated dicentric scoring is a useful tool in a large scale radiation accident as it enables high throughput screening of samples for fast triage of potentially exposed individuals.Abstract:
Mass casualty scenarios of radiation exposure require high throughput biological dosimetry techniques for population triage in order to rapidly identify individuals who require clinical treatment. The manual dicentric assay is a highly suitable technique, but it is also very time consuming and requires well trained scorers. In the framework of the MULTIBIODOSE EU FP7 project, semi-automated dicentric scoring has been established in six European biodosimetry laboratories. Whole blood was irradiated with a Co-60 gamma source resulting in 8 different doses between 0 and 4.5Gy and then shipped to the six participating laboratories. To investigate two different scoring strategies, cell cultures were set up with short term (2-3h) or long term (24h) colcemid treatment. Three classifiers for automatic dicentric detection were applied, two of which were developed specifically for these two different culture techniques. The automation procedure included metaphase finding, capture of cells at high resolution and detection of dicentric candidates. The automatically detected dicentric candidates were then evaluated by a trained human scorer, which led to the term 'semi-automated' being applied to the analysis. The six participating laboratories established at least one semi-automated calibration curve each, using the appropriate classifier for their colcemid treatment time. There was no significant difference between the calibration curves established, regardless of the classifier used. The ratio of false positive to true positive dicentric candidates was dose dependent. The total staff effort required for analysing 150 metaphases using the semi-automated approach was 2 min as opposed to 60 min for manual scoring of 50 metaphases. Semi-automated dicentric scoring is a useful tool in a large scale radiation accident as it enables high throughput screening of samples for fast triage of potentially exposed individuals. Furthermore, the results from the participating laboratories were comparable which supports networking between laboratories for this assay.read more
Citations
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Journal ArticleDOI
State-of-the-Art Advances in Radiation Biodosimetry for Mass Casualty Events Involving Radiation Exposure
Mary Sproull,Kevin Camphausen +1 more
TL;DR: The field of biodosimetry has advanced significantly beyond this original initiative, with expansion into the fields of genomics, proteomics, metabolomics and transcriptomics, and areas that still need to be addressed to achieve true field-deployment readiness are evaluated.
Journal ArticleDOI
Integration of new biological and physical retrospective dosimetry methods into EU emergency response plans – joint RENEB and EURADOS inter-laboratory comparisons
Elizabeth A. Ainsbury,Christophe Badie,Stephen Barnard,Grainne Manning,Jayne Moquet,Michael Abend,Ana Antunes,Lleonard Barrios,C. Bassinet,Christina Beinke,Emanuela Bortolin,Lily Bossin,Clare Bricknell,Kamil Brzóska,Iwona Buraczewska,Carlos Huertas Castaño,Zina Čemusová,Maria Christiansson,Santiago Mateos Cordero,Guillaume Cosler,Sara Della Monaca,François Desangles,Michael Discher,Inmaculada Domínguez,Sven Doucha-Senf,Jon Eakins,Paola Fattibene,Silvia Filippi,Monika Frenzel,Dimka Georgieva,Eric Gregoire,Kamile Guogyte,Valeria Hadjidekova,Ljubomira Hadjiiska,Rositsa Hristova,Maria Karakosta,Enikő Kis,Ralf Kriehuber,Jungil Lee,David Lloyd,Katalin Lumniczky,Fiona M. Lyng,Ellina Macaeva,Matthaeus Majewski,S. Vanda Martins,Stephen W.S. McKeever,Aidan D. Meade,Dinesh K. R. Medipally,Roberta Meschini,Radhia M'kacher,Octávia Monteiro Gil,Alegria Montero,Mercedes Moreno,Mihaela Noditi,Ursula Oestreicher,Dominik Oskamp,Fabrizio Palitti,Valentina Palma,Gabriel E. Pantelias,Jerome Pateux,Clarice Patrono,Gaetano Pepe,Matthias Port,María Jesús Prieto,Maria Cristina Quattrini,Roel Quintens,Michelle Ricoul,Laurence Roy,Laure Sabatier,Natividad Sebastià,Sergey Sholom,Sylwester Sommer,Albena Staynova,Sonja Strunz,Georgia I. Terzoudi,Antonella Testa,François Trompier,M. Valente,Olivier Van Hoey,Ivan Veronese,Andrzej Wojcik,Clemens Woda +81 more
TL;DR: Inter-comparisons of four new dosimetry methods proposed or developed show good agreement between the laboratories and methods within the expected levels of uncertainty, and demonstrate that there is a lot of potential for each of the candidate techniques.
Journal ArticleDOI
New tool for biological dosimetry: reevaluation and automation of the gold standard method following telomere and centromere staining.
Radhia M'kacher,Elie Maalouf,Michelle Ricoul,Leonhard Heidingsfelder,Eric Laplagne,Corina Cuceu,William M. Hempel,Bruno Colicchio,Alain Dieterlen,Laure Sabatier +9 more
TL;DR: The use of TC staining has permitted a reevaluation of the dose-response curve and the highly efficient automation of the scoring process, marking a new step in the management and follow-up of populations exposed to genotoxic agents including ionizing radiation.
Journal ArticleDOI
Inter- and intra-laboratory comparison of a multibiodosimetric approach to triage in a simulated, large scale radiation emergency
Elizabeth A. Ainsbury,Jenna Al-hafidh,Ainars Bajinskis,Stephen Barnard,Joan Francesc Barquinero,Christina Beinke,Virginie de Gelder,Eric Gregoire,Alicja Jaworska,Carita Lindholm,David Lloyd,Jayne Moquet,Reetta Nylund,Ursula Oestreicher,Sandrine Roch-Lefèvre,Kai Rothkamm,Horst Romm,Harry Scherthan,Sylwester Sommer,H. Thierens,Charlot Vandevoorde,Anne Vral,Andrzej Wojcik +22 more
TL;DR: The data demonstrate that the MULTIBIODOSE approach of applying multiparametric tools to radiation emergencies is valid and effective.
Journal ArticleDOI
Zero-inflated regression models for radiation-induced chromosome aberration data: A comparative study
María Oliveira,Jochen Einbeck,Manuel Higueras,Manuel Higueras,Elizabeth A. Ainsbury,Pedro Puig,Kai Rothkamm,Kai Rothkamm +7 more
TL;DR: Different methodologies for analyzing cytogenetic chromosomal aberrations datasets are compared, with special focus on zero-inflated Poisson and zero- inflated negative binomial models.
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