Journal ArticleDOI
Biophysical aspects of lysozyme adduct with monocrotophos
TLDR
The potential of lysozyme to be used as a sensitive biomarker to monitor exposure levels to the commonly used organophosphorus pesticide monocrotophos is evaluated to understand the adduct formation mechanism at a molecular level.Abstract:
The present study on in vitro formation and characterization of lysozyme adduct with monocrotophos (MP) evaluates the potential of lysozyme to be used as a sensitive biomarker to monitor exposure levels to the commonly used organophosphorus pesticide monocrotophos. Crystallization of lysozyme protein adduct with monocrotophos was also undertaken to understand the adduct formation mechanism at a molecular level. The binding of organophosphorus pesticides to lysozyme is one of the key steps in their mutagenicity. The formation and structural characterization of lysozyme adduct with monocrotophos was done using MALDI-TOFMS, fluorescence, UV/Vis spectroscopy, circular dichroism, and X-ray diffraction studies. We report the crystal structure of lysozyme adduct with monocrotophos at 1.9 A. It crystallized in the P43 space group with two monomers in one asymmetric unit having one molecule of monocrotophos bound to each protein chain. The results proved that the fluorescence quenching of lysozyme by monocrotophos is due to binding of monocrotophos with a tryptophan residue of lysozyme. Monocrotophos interacts most strongly with the Trp-108 and Asp-52 of lysozyme. The interactions of the monocrotophos molecule with the lysozyme suggest the formation of a stable adduct. In addition, the alteration of lysozyme secondary structure in the presence of monocrotophos was confirmed by circular dichroism and fluorescence inhibition of lysozyme by increasing monocrotophos and UV/Vis spectrophotometry. The formation of lysozyme adduct with monocrotophos was confirmed by MALDI-TOFMS.read more
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Policresulen, a novel NS2B/NS3 protease inhibitor, effectively inhibits the replication of DENV2 virus in BHK-21 cells
TL;DR: Policresulen is a potent inhibitor ofDENV2 NS2B/NS3 protease that inhibits DENV2 replication in BHK-21 cells and provides useful hints for designing new type of inhibitors against the protease.
Journal ArticleDOI
Relationships of human α/β hydrolase fold proteins and other organophosphate-interacting proteins
TL;DR: This review sitsuate the α/β hydrolase fold proteins among the distinctively folded proteins known to interact with OPs, in particular the different lipases, peptidases, and enzymes hydrolyzing OPs.
Journal ArticleDOI
Binding studies for the interaction between hazardous organophosphorus compound phosmet and lysozyme: Spectroscopic and In-silico analyses
Lajpreet Kaur,Afreen Jahan Rahman,Anju Aji Sarita Karthikeyan Sarabjit Singh,Mallika Pathak,Anup Kumar Datta,Rahul Singhal,Himanshu Ojha +6 more
TL;DR: In this article , the binding of phosmet with lysozyme was investigated to understand the toxicology and the transport of Phosmet using various spectroscopic techniques, molecular modeling and enzymatic assay.
Journal ArticleDOI
Analyzing organophosphate pesticide-serum albumin binding interaction: a combined STD NMR and molecular docking study
TL;DR: In Vitro analysis of the interaction of organophosphate pesticides (OP) with bovine serum albumin (BSA) is crucial to understand their potential effects at the molecular level and Saturation Transfer Difference NMR experiments in conjunction with molecular docking studies revealed a high binding affinity of OP-BSA complexes through non-covalent interaction.
Journal ArticleDOI
Deposition, Bioaccumulation and Depletion of Organophosphate Triesters (tri-OPEs) and Their Organophosphate Diester Metabolites (di-OPEs) From Feed to Laying Hens’ Eggs
TL;DR: In this paper , the authors fed 135 laying hens (control group, low-level group (LLG) and high level group (HLG) with chlorinated alkyl-, alkyal- and aryl-tri-OPEs for 14 days and followed by a 28-day depuration period to investigate in ovo transfer.
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