Journal ArticleDOI
Bioprocess development for the production of a recombinant MUC1 fusion protein expressed by CHO-K1 cells in protein-free medium.
Thomas Link,Malin Bäckström,Ros Graham,Ruth Essers,K Zörner,Jochem Gätgens,J. Burchell,Joyce Taylor-Papadimitriou,Gunnar C. Hansson,Thomas Noll +9 more
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TLDR
A bioprocess for the expression of a recombinant MUC1 fusion protein with a cancer associated glycosylation in CHO-K1 cells has been developed, indicating that the desired glycoforms were obtained and suggesting that the recombinantMUC1 protein could be tested for use in immunotherapy.About:
This article is published in Journal of Biotechnology.The article was published on 2004-05-13. It has received 77 citations till now. The article focuses on the topics: Cell culture & Fusion protein.read more
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Journal ArticleDOI
Retargeting of human T cells to tumor-associated MUC1: the evolution of a chimeric antigen receptor.
Scott Wilkie,Gianfranco Picco,Julie Foster,David M. Davies,Sylvain Julien,Lucienne Cooper,Sefina Arif,Stephen J. Mather,Joyce Taylor-Papadimitriou,Joy Burchell,John Maher +10 more
TL;DR: Together, these data demonstrate for the first time that the near-ubiquitous MUC1 tumor Ag can be targeted using CAR-grafted T cells.
Journal ArticleDOI
Process parameter shifting: Part I. Effect of DOT, pH, and temperature on the performance of Epo-Fc expressing CHO cells cultivated in controlled batch bioreactors.
Evelyn Trummer,Katharina Fauland,Silke Seidinger,Kornelia Schriebl,Christine Lattenmayer,Renate Kunert,Karola Vorauer-Uhl,Robert Weik,Nicole Borth,Hermann Katinger,Dethardt Müller +10 more
TL;DR: The reduction of cultivation temperature and the reduction of (external) pH are found to exert the most significant effects on process performance by mainly reducing cell growth and metabolism.
Journal ArticleDOI
The ST6GalNAc-I sialyltransferase localizes throughout the golgi and is responsible for the synthesis of the tumor- associated sialyl-Tn O-glycan in human breast cancer
Robert Sewell,Malin Bäckström,Martin Dalziel,Steven Gschmeissner,Hasse Karlsson,Thomas Noll,Jochem Gätgens,Henrik Clausen,Gunnar C. Hansson,Joy Burchell,Joyce Taylor-Papadimitriou +10 more
TL;DR: The development of a Chinese hamster ovary cell line expressing Muc1 and ST6GalNAc-I allowed the large scale production of MUC1 carrying 83% sialyl-Tn O-glycans, and the availability of large quantities of this MUC 1 glycoform will allow the evaluation of its efficacy as an immunogen for immunotherapy of M UC1/STn-expressing tumors.
Journal ArticleDOI
Expression of Tumor-Associated Differentiation Antigens, MUC1 Glycoforms and CEA, in Human Thymic Epithelial Cells: Implications for Self-Tolerance and Tumor Therapy
Silvie Cloosen,Janna Arnold,Marco Thio,Gerard M. J. Bos,Bruno Kyewski,Wilfred T. V. Germeraad +5 more
TL;DR: The findings imply that MUC1 and CEA are amenable to central tolerance induction, which might, however, be incomplete in case of tumor cell-restricted MUC2 glycoforms, and knowledge of these subtleties in promiscuous gene expression may assist the selection of T cell tumor vaccines for clinical trials.
Journal ArticleDOI
Recombinant Tumor-Associated MUC1 Glycoprotein Impairs the Differentiation and Function of Dendritic Cells
Aurelia Rughetti,Ilenia Pellicciotta,Mauro Biffoni,Malin Bäckström,Thomas Link,Eric P. Bennet,Henrik Clausen,Thomas Noll,Gunnar C. Hansson,Joy Burchell,Luigi Frati,Joyce Taylor-Papadimitriou,Marianna Nuti +12 more
TL;DR: The altered DC differentiation and Ag presentation function induced by the soluble sialylated tumor-associated mucin may represent a mechanism by which epithelial tumors can escape immunosurveillance.
References
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Role of HIF-1alpha in hypoxia-mediated apoptosis, cell proliferation and tumour angiogenesis.
Peter Carmeliet,Yuval Dor,Jean-Marc Herbert,Dai Fukumura,Koen Brusselmans,Mieke Dewerchin,Michal Neeman,Françoise Bono,Rinat Abramovitch,Patrick H. Maxwell,Cameron J. Koch,Peter J. Ratcliffe,Lieve Moons,Rakesh K. Jain,Desire Collen,Eli Keshert +15 more
TL;DR: It is shown that hypoxia and hypoglycaemia reduce proliferation and increase apoptosis in wild-type (Hif-1α+/+) embryonic stem (ES) cells, but not in ES cells with inactivated HIF-1 α genes (HIF- 1α−/−), suggesting that there are at least two different adaptive responses to being deprived of oxygen and nutrients.
Journal ArticleDOI
Molecular cloning and expression of human Tumor-associated Polymorphic Epithelial Mucin
Sandra Gendler,Carole A. Lancaster,Joyce Taylor-Papadimitriou,Trevor Duhig,Nigel Peat,Joy Burchell,Lucy Pemberton,El-Nasir Lalani,David Wilson +8 more
TL;DR: The full sequence for PEM, as deduced from cDNA sequences, is reported, with length variations in the tandem repeat result in PEM being an expressed variable number tandem repeat locus.
Journal ArticleDOI
Transcription factor HIF-1 is a necessary mediator of the pasteur effect in mammalian cells.
Tiffany N. Seagroves,Heather E. Ryan,Han Lu,Bradly G. Wouters,Merrill Knapp,Pierre Thibault,Keith R. Laderoute,Randall S. Johnson +7 more
TL;DR: HIF-1 activation is an essential control element of the metabolic state during hypoxia; this requirement has important implications for the regulation of cell growth during development, angiogenesis, and vascular injury.
Journal ArticleDOI
Industrial choices for protein production by large-scale cell culture.
Lily Chu,David K. Robinson +1 more
TL;DR: Traditional barriers to large-scale mammalian culture have now been addressed, with the standard stirred-tank reactor emerging as industry's technology of choice and low-volume reactor technology remains diversified.
Journal ArticleDOI
Getting the glycosylation right: implications for the biotechnology industry.
TL;DR: The impact of advances in cloning of glycosyltransferase enzymes, in understanding the varied biological functions of carbohydrates, and in the accurate analysis of glycoprotein heterogeneity are discussed.