Journal ArticleDOI
Cell-laden microfibers fabricated using μL cell-suspension.
TLDR
Investigations on the morphology and function of the encapsulated cells show the viability of the cells is not significantly affected by the fabrication process, and indicate the potential of using the method to perform quantitative assays on fiber-shaped tissues, while reducing the overall material- and time- consumption.Abstract:
Current microfluidic methods for cell-laden microfiber fabrication generally require larger than 100 μl of cell-suspensions. Since some 'rare' cells can be only acquired in small amounts, the preparation of >100 μl cell-suspensions with high-cell density can be both expensive and time consuming. Here, we present a facile method capable of fabricating cell-laden microfibers using small-volume cell-suspensions. The method utilizes a 3D-printed coaxial microfluidic device featured with a 'luer-lock inlet' to effectively load cell-suspensions in a deterministic volume (down to 5 μl) with a low sample-loss. In experiments, we demonstrate the formation of fibrous tissues consisting of various kinds of cells. Investigations on the morphology and function of the encapsulated cells show the viability of the cells is not significantly affected by the fabrication process, and also indicate the potential of using our method to perform quantitative assays on fiber-shaped tissues, while reducing the overall material and time consumption.read more
Citations
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Organ Printing: Tissue Spheroids as Building Blocks
Vladimir Mironov,Jing Zhang,Carmine Gentile,K Brakke,Thomas C. Trusk,Karoly Jakab,Gabor Forgacs,Vladimirs Kasjanovs,Richard P. Visconti,Roger R. Markwald +9 more
TL;DR: Organ printing can be defined as layer-by-layer additive robotic biofabrication of three-dimensional functional living macrotissues and organ constructs using tissue spheroids as building blocks.
Journal ArticleDOI
Microfluidic Formulation of Topological Hydrogels for Microtissue Engineering
TL;DR: This work reviews the available microfluidic fabrication methods by exploiting various cross-linking mechanisms and various routes toward compartmentalization and critically discusses the available tissue-specific applications.
Journal ArticleDOI
Luer-lock valve: A pre-fabricated pneumatic valve for 3D printed microfluidic automation
Minghao Nie,Shoji Takeuchi +1 more
TL;DR: A type of prefabricated polydimethylsiloxane valves, named the "Luer-lock" valve, which can be incorporated in 3D printed microfluidic devices utilizing the Luer-lock mechanism, which has the potential to be easily adopted by researchers around the globe.
Journal ArticleDOI
Electrospinning and Cell Fibers in Biomedical Applications.
Qilong Zhao,Xuemin Du,Min Wang +2 more
TL;DR: In this paper , a concise review is provided of the "bottom-up" biomanufacturing technologies and materials usable for fabricating cell fibers, with an emphasis on electrospinning that can effectively and efficiently produce thin cell fibers.
Journal ArticleDOI
Millimeter-thick 3D tissues constructed by densely cellularized core–shell microfluidic bioprinting
TL;DR: In this article , a microfluidic bioprinting method was proposed to fabricate 3D tissue constructs consisting of core-shell microfibers where extracellular matrices and cells can be encapsulated within the core of the fibers.
References
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Journal ArticleDOI
Organ printing: Tissue spheroids as building blocks☆
Vladimir Mironov,Richard P. Visconti,Vladimir Kasyanov,Gabor Forgacs,Christopher J. Drake,Roger R. Markwald +5 more
TL;DR: Organ printing is a new emerging enabling technology paradigm which represents a developmental biology-inspired alternative to classic biodegradable solid scaffold-based approaches in tissue engineering.
Organ Printing: Tissue Spheroids as Building Blocks
Vladimir Mironov,Jing Zhang,Carmine Gentile,K Brakke,Thomas C. Trusk,Karoly Jakab,Gabor Forgacs,Vladimirs Kasjanovs,Richard P. Visconti,Roger R. Markwald +9 more
TL;DR: Organ printing can be defined as layer-by-layer additive robotic biofabrication of three-dimensional functional living macrotissues and organ constructs using tissue spheroids as building blocks.
Journal ArticleDOI
Metre-long cell-laden microfibres exhibit tissue morphologies and functions
Hiroaki Onoe,Teru Okitsu,Akane Itou,Midori Kato-Negishi,Riho Gojo,Daisuke Kiriya,Koji Sato,Shigenori Miura,Shintaroh Iwanaga,Kaori Kuribayashi-Shigetomi,Yukiko T. Matsunaga,Yuto Shimoyama,Shoji Takeuchi +12 more
TL;DR: Fibres encapsulating primary pancreatic islet cells and transplanted through a microcatheter into the subrenal capsular space of diabetic mice normalized blood glucose concentrations for about two weeks and may find use as templates for the reconstruction of fibre-shaped functional tissues that mimic muscle fibres, blood vessels or nerve networks in vivo.
Journal ArticleDOI
Centrifugal microfluidics for biomedical applications
Robert Gorkin,Jiwoon Park,Jonathan Siegrist,Mary Amasia,Beom Seok Lee,Jong Myeon Park,Jong Myeon Park,Jin-Tae Kim,Hanshin Kim,Marc J. Madou,Marc J. Madou,Yoon-Kyoung Cho +11 more
TL;DR: An in-depth review of the centrifugal microfluidic platform, while highlighting recent progress in the field and outlining the potential for future applications, is presented in this paper.
Book
Cell Biology by the Numbers
Ron Milo,Rob Phillips +1 more
TL;DR: The Path to Biological Numeracy 1. Size and Geometry 2. Concentrations and Absolute Numbers 3. Energies and Forces 4. Rates and Duration 5. Information & Errors 6. A Quantitative Miscellany Epilogue