Characterization of filamentous phages of Vibrio cholerae O139 and O1
Masahiko Ehara,Shoichi Shimodori,Fumiko Kojima,Yoshio Ichinose,Toshiya Hirayama,M. John Albert,Krongkaew Supawat,Yasuko Honma,Masaaki Iwanaga,Kazunobu Amako +9 more
Reads0
Chats0
TLDR
In this paper, a collection of Vibrio cholerae O139 strains were analyzed to determine whether filamentous phages are produced in their culture supernatants, and whether any replicative form of DNA is detectable in cell lysates.Abstract:
We have analyzed our collection of Vibrio cholerae O139 strains to determine whether filamentous phages are produced in their culture supernatants, and whether any replicative form of DNA is detectable in cell lysates. Two types of filamentous phage, designated fs1 (6.4 kb) and fs2 (8.5 kb), were found in strains of Vibrio cholerae O139, fs1 was commonly produced from clinical isolates of Vibrio cholerae O1. Infectious particles (filamentous phages) were inducible by subculture, mitomycin C, and cultivation in a ligated ileal loop of a rabbit. Type 4 fimbriae of Vibrio cholerae O1 sensitive to d-glucose and d-mannose were suggested to be receptors for fs1 and fs2. The genome of fs1 was revealed to encode a potential new enterotoxin homologous to zonula occludens toxin. Clarification of the relation of type 4 fimbriae and these filamentous phages will provide a new understanding of the colonization of Vibrio cholerae O1 and O139. Thus the presence of a new enterotoxin encoded by the genome of filamentous phage like fs1 may clarify the pathogenesis of cholera toxin negative clinical isolates of Vibrio cholerae O1 and non-O1. Our findings combined with the earlier report by Ehara et al. [Microbio. Immunol. 37 (1993) 679–688] suggest that type 4 fimbriae of Vibrio cholerae O1 are important for the development of an effective vaccine against cholera.read more
Citations
More filters
Journal ArticleDOI
Filamentous phage structure, infection and assembly
TL;DR: The structural model of filamentous phage derived by X-ray fibre diffraction is supported by spectroscopic and genetic experiments and suggests models for virus infection and assembly.
Journal ArticleDOI
Genetic and phenotypic diversity of quorum-sensing systems in clinical and environmental isolates of Vibrio cholerae.
Adam Joelsson,Zhi Liu,Jun Zhu +2 more
TL;DR: It is found that a functional quorum-sensing system conferred a survival advantage on bacteria in biofilms when the bacteria were exposed to seawater, though quorum sensing was less important to survival in a planktonic state under the same conditions.
Journal ArticleDOI
Phage-bacterial interactions in the evolution of toxigenic Vibrio cholerae.
TL;DR: The complex array of natural phenomena driving the evolution of pathogenic V. cholerae includes, among other factors, phages that either participate in horizontal gene transfer or in a bactericidal selection process favoring the emergence of new clones of V. Cholerae.
Journal ArticleDOI
Satellite phage TLCφ enables toxigenic conversion by CTX phage through dif site alteration
TL;DR: It is found that acquisition and chromosomal integration of the TLC-Knφ1 genome restored a perfect dif site and normal morphology to V. cholerae wild-type and mutant strains with dif− filamentation phenotypes.
Journal ArticleDOI
VGJφ, a Novel Filamentous Phage of Vibrio cholerae, Integrates into the Same Chromosomal Site as CTXφ
Javier Campos,Eriel Martínez,Edith Suzarte,Boris L. Rodríguez,Karen Marrero,Yussuan Silva,Talena Ledón,Ricardo Del Sol,Rafael Fando +8 more
TL;DR: A model in which repeated integration events with different phages might have contributed to the evolution of the CTX chromosomal region in V. cholerae El Tor is proposed.
References
More filters
Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
TL;DR: A method has been devised for the electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets that results in quantitative transfer of ribosomal proteins from gels containing urea.
Journal ArticleDOI
Lysogenic conversion by a filamentous phage encoding cholera toxin.
TL;DR: The emergence of toxigenic V. cholerae involves horizontal gene transfer that may depend on in vivo gene expression, and is shown here to be encoded by a filamentous bacteriophage (designated CTXΦ), which is related to coliphage M13.
Journal ArticleDOI
Vibrio cholerae produces a second enterotoxin, which affects intestinal tight junctions.
Alessio Fasano,B Baudry,D W Pumplin,Steven S. Wasserman,Ben D. Tall,Julian M. Ketley,J B Kaper +6 more
TL;DR: In this paper, the authors examined culture supernatants of V. cholerae strains, both CT-positive and CT-negative, and found a toxin that increases the permeability of the small intestinal mucosa by affecting the structure of the intercellular tight junction, or zonula occludens.
Related Papers (5)
Lysogenic conversion by a filamentous phage encoding cholera toxin.
Filamentous phage integration requires the host recombinases XerC and XerD
DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae
John F. Heidelberg,Jonathan A. Eisen,William C. Nelson,Rebecca A. Clayton,Michelle L. Gwinn,Robert J. Dodson,Daniel H. Haft,Erin Hickey,Jeremy Peterson,Lowell Umayam,Steven R. Gill,Karen E. Nelson,Timothy D. Read,Hervé Tettelin,Delwood Richardson,Maria D. Ermolaeva,Jessica Vamathevan,Steven Bass,Haiying Qin,Ioana Dragoi,Patrick Sellers,Lisa McDonald,Teresa Utterback,Robert D. Fleishmann,William C. Nierman,Owen White,Steven L. Salzberg,Hamilton O. Smith,Rita R. Colwell,Rita R. Colwell,John J. Mekalanos,J. Craig Venter,Claire M. Fraser +32 more