Chromatin Boundaries in Budding Yeast: The Nuclear Pore Connection
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TLDR
The data demonstrate that physical tethering of genomic loci to the NPC can dramatically alter their epigenetic activity.About:
This article is published in Cell.The article was published on 2002-05-31 and is currently open access. It has received 371 citations till now. The article focuses on the topics: Chromatin & Chromatin immunoprecipitation.read more
Citations
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Histone and chromatin cross-talk.
TL;DR: Different layers of cross-talk between several components of this complex regulatory system are emerging, and these epigenetic circuits are the focus of this review.
Journal ArticleDOI
Beyond the sequence : Cellular organization of genome function
TL;DR: The functional relevance of spatial and temporal genome organization at three hierarchical levels: the organization of nuclear processes, the higher-order organization of the chromatin fiber, and the spatial arrangement of genomes within the cell nucleus are discussed.
Journal ArticleDOI
Proteomic analysis of the mammalian nuclear pore complex
TL;DR: A proteomic analysis to identify and classify all of its protein components and defines the proteome of the mammalian NPC for the first time and paves the way for a more detailed characterization of NPC structure and function.
Journal ArticleDOI
Chromatin immunoprecipitation and microarray-based analysis of protein location.
TL;DR: These protocols require approximately 1 week to complete once sufficient numbers of cells have been obtained, and have been used to produce robust, high-quality ChIP-chip results in many different cell and tissue types.
References
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Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae
Mark S. Longtine,Amos Mckenzie,Douglas J. Demarini,Nirav Shah,Achim Wach,Arndt Brachat,Peter Philippsen,John R. Pringle +7 more
TL;DR: A new set of plasmids that serve as templates for the PCR synthesis of fragments that allow a variety of gene modifications that should further facilitate the rapid analysis of gene function in S. cerevisiae.
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A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance.
TL;DR: Mutations at the URA3 locus of Saccharomyces cerevisiae can be obtained by a positive selection, based on the loss of orotidine-5′-phosphate decarboxylase activity, and seems applicable to a variety of eucaryotic and procaryotic cells.
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Chromosome territories, nuclear architecture and gene regulation in mammalian cells.
TL;DR: The emerging view is that chromosomes are compartmentalized into discrete territories and the location of a gene within a chromosome territory seems to influence its access to the machinery responsible for specific nuclear functions, such as transcription and splicing.
Journal ArticleDOI
Transport between the cell nucleus and the cytoplasm.
Dirk Görlich,Ulrike Kutay +1 more
TL;DR: A focus of this review is nuclear export of messenger RNA, which apparently largely relies on export mediators distinct from importin beta-related factors.
Nuclear architecture and gene regulation in mammalian cells
TL;DR: The emerging view is that chromosomes are compartmentalized into discrete territories, and the location of a gene within a chromosome territory seems to influence its access to the machinery responsible for specific nuclear functions, such as transcription and splicing.
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