Journal ArticleDOI
Co-delivery of Doxorubicin and Ceramide in a Liposomal Formulation Enhances Cytotoxicity in Murine B16BL6 Melanoma Cell Lines
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This study reports co-delivery of doxorubicin (DOX) and ceramide in a liposomal system in B16BL6 melanoma cell lines for enhanced cytotoxic effects and provides basis for developing a co-Delivery system of DOX and ceramides for lowering the dose and dose-related side effects ofDOX for the treatment of melanoma.Abstract:
This study reports co-delivery of doxorubicin (DOX) and ceramide in a liposomal system in B16BL6 melanoma cell lines for enhanced cytotoxic effects. Different types of ceramides (C6-ceramide, C8-ceramide, and C8-glucosylceramide) and lipids (1,2-dioleoyl-3-trimethylammonium-propane (DOTAP), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine (DSPE)) were considered in the preparation of liposomes. DOX was encapsulated within liposome, and ceramide was used as the component of the lipid bilayer. The formulations were optimized for size and size distribution, zeta potential, and DOX encapsulation efficiency (EE). Cytotoxic effect on B16BL6 melanoma cell lines was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The ceramide based liposome formulations generally provided a mean diameter 90% DOX EE. Co-delivery of DOX and C8-ceramide with DOTAP liposomes demonstrated significantly higher cytotoxicity as compared to DOX liposomes without ceramide (P < 0.001), and also showed enhanced cellular uptake by B16BL6 cell lines. This study provides basis for developing a co-delivery system of DOX and ceramide for lowering the dose and dose-related side effects of DOX for the treatment of melanoma.read more
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Journal ArticleDOI
Outstanding Graphene Quantum Dots from Carbon Source for Biomedical and Corrosion Inhibition Applications: A Review
Badreah A. Al Jahdaly,Mohamed Farouk Elsadek,Badreldin Mohamed Ahmed,Mohamed Fawzy Farahat,Mohamed M. Taher,Ahmed Khalil +5 more
TL;DR: In this article, a review of GQD-based materials in the fields of sensing, bio-imaging, energy storage, and corrosion inhibitors is presented, which emphasizes possible eco-friendly and sustainable methodologies that have been successfully employed in the fabrication of graphene quantum dots.
Journal ArticleDOI
What sustains the multidrug resistance phenotype beyond ABC efflux transporters? Looking beyond the tip of the iceberg
Teodora Alexa-Stratulat,Milica Pešić,Ana Čipak Gašparović,Ioannis P. Trougakos,Chiara Riganti +4 more
TL;DR: The aim of this review is to discuss the key molecular mechanisms of cellular reprogramming that induce and maintain MDR, beyond the presence of MDR efflux transporters and suggest that mapping the specific adaptation pathways underlying cell survival in response to stress and targeting these pathways with potent pharmacologic agents may be a new approach to enhance therapeutic efficacy against MDR tumors.
Journal ArticleDOI
Nanotechnology approaches in the current therapy of skin cancer.
Lívia Neves Borgheti-Cardoso,Juliana Santos Rosa Viegas,Ana Vitoria Pupo Silvestrini,Angelo Luis Caron,Fabíola Silva Garcia Praça,Marcelo Kravicz,Maria Vitória Lopes Badra Bentley +6 more
TL;DR: In this review, nanotechnological tools for skin cancer will be summarized and discussed, and the use of NPs to deliver chemotherapeutics, immune and vaccine molecules and nucleic acids will be reviewed and discussed.
Journal ArticleDOI
Resveratrol-loaded nanomedicines for cancer applications.
Manjusha Annaji,Ishwor Poudel,Sai H.S. Boddu,Robert D. Arnold,Amit K. Tiwari,R. Jayachandra Babu +5 more
TL;DR: In this paper, the physicochemical properties of resveratrol, the therapeutic potential of nano-formulations, and the anticancer activity of encapsulated nanoparticles on various malignancies such as skin, breast, prostate, colon, liver, ovarian, and lung cancers (focusing on both in vitro and in vivo studies).
Journal ArticleDOI
Sphingolipid/Ceramide Pathways and Autophagy in the Onset and Progression of Melanoma: Novel Therapeutic Targets and Opportunities.
TL;DR: This review illustrates the role of autophagy in tumor progression and resistance to therapy, focusing on molecular targets for future drugs.
References
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