Journal ArticleDOI
Competing endogenous RNA network crosstalk reveals novel molecular markers in colorectal cancer.
Nehal Samir,Marwa Matboli,Hanaa El-Tayeb,Ahmed El‐Tawdi,Mohmed K. Hassan,Amr A. Waly,Hesham A. E. EL‐Akkad,Mohamed G. Ramadan,Tarek N. Al‐Belkini,Sherif F. El-Khamisy,Farid El-Asmar +10 more
TLDR
The results revealed that the three RNAs‐based biomarker network, long non‐coding intergenic RNA‐[lncRNA RP11‐909B2.1], Homo sapiens microRNA‐595 [hsa‐mi RNA‐595], and L3MBTL1 mRNA), had high sensitivity and specificity for discriminating CRC from healthy controls and also from benign colorectal neoplasm.Abstract:
The competing endogenous RNA networks play a pivotal role in cancer diagnosis and progression. Novel properstrategies for early detection of colorectal cancer (CRC) are strongly needed. We investigated a novel CRC-specific RNA-based integrated competing endogenous network composed of lethal3 malignant brain tumor like1 (L3MBTL1) gene, long non-coding intergenic RNA- (lncRNA RP11-909B2.1) and homo sapiens microRNA-595 (hsa-miRNA-595) using in silico data analysis. RT-qPCR-based validation of the network was achieved in serum of 70 patients with CRC, 40 patients with benign colorectal neoplasm, and 20 healthy controls. Moreover, in cancer tissues of 20 of the 70 CRC cases were involved in the study. The expression of RNA-based biomarker network in both CRC and adjacent non-tumor tissues and their correlation with the serum levels of this network members was investigated. Lastly, the expression levels of the chosen ceRNA was verified in CRC cell line. Our results revealed that the three RNAs-based biomarker network (long non-coding intergenic RNA-[lncRNA RP11-909B2.1], Homo sapiens microRNA-595 [hsa-miRNA-595], and L3MBTL1 mRNA), had high sensitivity and specificity for discriminating CRC from healthy controls and also from benign colorectal neoplasm. The data suggest that among these three RNAs, serum lncRNA RP11-909B2.1 could be a promising independent prognostic factors in CRC. The circulatory RNA based biomarker panel can act as potential biomarker for CRC diagnosis and prognosis.read more
Citations
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MicroRNAs: Target Recognition and Regulatory Functions
TL;DR: In this article, a review outlines the current understanding of miRNA target recognition in animals and discusses the widespread impact of miRNAs on both the expression and evolution of protein-coding genes.
Journal ArticleDOI
MicroRNA hsa-miR-623 directly suppresses MMP1 and attenuates IL-8-induced metastasis in pancreatic cancer
Y.-m. Chen,Siqi Peng,Hong Cen,Yujing Lin,Chumei Huang,Yinting Chen,Hong Shan,Yonghui Su,Linjuan Zeng +8 more
TL;DR: Hsa-miR-623 represents a novel adjuvant therapeutic target to prevent metastasis in pancreatic cancer and is screened as a candidate miRNA that interacts with the MMP1 transcript.
Journal ArticleDOI
Dynamic liquid biopsy components as predictive and prognostic biomarkers in colorectal cancer
Afsheen Raza,Abdul Quaiyoom Khan,Varghese Inchakalody,Sarra Mestiri,Z. S. K. M. Yoosuf,Takwa Bedhiafi,Dina M. Abo El-Ella,Nassiba Taib,Shereena Hydrose,Shayista Akbar,Queenie Fernandes,Lobna Al-Zaidan,Roopesh Krishnankutty,Maysaloun Merhi,Shahab Uddin,Said Dermime +15 more
TL;DR: A review of liquid biopsy biomarkers for colorectal cancer can be found in this paper , where the authors aim to provide updated information on various novel liquid-biopsy markers such as a) oral microbiota related bacterial network b) gut microbiome-associated serum metabolites c) PIWI-interacting RNAs, microRNA(miRNAs), long non-coding RNAs), circular RNAs (circRNAs) and d) circulating tumor DNAs (ctDNA) and circulating tumor cells (CTC) and their applicability for personalized management of CRC.
Journal ArticleDOI
Dynamic liquid biopsy components as predictive and prognostic biomarkers in colorectal cancer
Afsheen Raza,Abdul Q. Khan,Varghese Inchakalody,Sarra Mestiri,Z. S. K. M. Yoosuf,Takwa Bedhiafi,Dina M. Abo El-Ella,Nassiba Taib,Shereena Hydrose,Shayista Akbar,Queenie Fernandes,Lobna Al-Zaidan,Roopesh Krishnankutty,Maysaloun Merhi,Shahab Uddin,Said Dermime +15 more
TL;DR: A review of liquid biopsy biomarkers for colorectal cancer can be found in this article , where the authors aim to provide updated information on various novel liquid-biopsy markers such as a) oral microbiota related bacterial network b) gut microbiome-associated serum metabolites c) PIWI-interacting RNAs, microRNA(miRNAs), long non-coding RNAs), circular RNAs (circRNAs) and d) circulating tumor DNAs (ctDNA) and circulating tumor cells (CTC) and their applicability for personalized management of CRC.
Journal ArticleDOI
The long non-coding RNA HOXA11-AS promotes epithelial mesenchymal transition by sponging miR-149-3p in Colorectal Cancer.
Dong Chen,Min Zhang,Jian Ruan,Xiaolin Li,Saisai Wang,Xiaofei Cheng,Huiying Zhao,Ying Zeng,Jingjing Liu,Kang-Xin He,Peng Zhao +10 more
TL;DR: It is demonstrated that high expression of HOXA11-AS is correlated with CRC progression and poor prognosis and may promote metastasis via EMT by modulating miR-149-3p.
References
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MicroRNAs: Target Recognition and Regulatory Functions
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