Comprehensive analysis of the ICEN (Interphase Centromere Complex) components enriched in the CENP-A chromatin of human cells.
Hiroshi Izuta,Masashi Ikeno,Nobutaka Suzuki,Takeshi Tomonaga,Naohito Nozaki,Chikashi Obuse,Yasutomo Kisu,Naoki Goshima,Fumio Nomura,Nobuo Nomura,Kinya Yoda +10 more
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TLDR
Analysis of seven ICEN components with unknown function suggest that the seven components of the ICEN complex are predominantly localized at the centromeres and are required for kinetochore function perhaps through or not through loading of CENP‐H and hMis6 onto the centromere.Abstract:
The centromere is a chromatin structure essential for correct segregation of sister chromatids, and defects in this region often lead to aneuploidy and cancer. We have previously reported purification of the interphase centromere complex (ICEN) from HeLa cells, and have demonstrated the presence of 40 proteins (ICEN1-40), along with CENP-A, -B, -C, -H and hMis6, by proteomic analysis. Here we report analysis of seven ICEN components with unknown function. Centromere localization of EGFP-tagged ICEN22, 24, 32, 33, 36, 37 and 39 was observed in transformant cells. Depletion of each of these proteins by short RNA interference produced abnormal metaphase cells carrying misaligned chromosomes and also produced cells containing aneuploid chromosomes, implying that these ICEN proteins take part in kinetochore functions. Interestingly, in the ICEN22, 32, 33, 37 or 39 siRNA-transfected cells, CENP-H and hMis6 signals disappeared from all the centromeres in abnormal mitotic cells containing misaligned chromosomes. These results suggest that the seven components of the ICEN complex are predominantly localized at the centromeres and are required for kinetochore function perhaps through or not through loading of CENP-H and hMis6 onto the centromere.read more
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Molecular architecture of the kinetochore–microtubule interface
Iain M. Cheeseman,Arshad Desai +1 more
TL;DR: The kinetochore is composed of a number of conserved protein complexes that direct its specification and assembly, bind to spindle microtubules and regulate chromosome segregation.
Journal ArticleDOI
Centromere-Specific Assembly of CENP-A Nucleosomes Is Mediated by HJURP
Daniel R. Foltz,Daniel R. Foltz,Lars E.T. Jansen,Lars E.T. Jansen,Aaron O. Bailey,John R. Yates,Emily A. Bassett,Stacey Wood,Ben E. Black,Don W. Cleveland,Don W. Cleveland +10 more
TL;DR: The proteins responsible for assembly of human CENP-A into centromeric nucleosomes during the G1 phase of the cell cycle are shown here to be distinct from the chromatin assembly factors previously shown to load other histone H3 variants.
Journal ArticleDOI
HJURP is a cell-cycle-dependent maintenance and deposition factor of CENP-A at centromeres
Elaine M. Dunleavy,Danièle Roche,Hideaki Tagami,Nicolas Lacoste,Dominique Ray-Gallet,Yusuke Nakamura,Yataro Daigo,Yoshihiro Nakatani,Geneviève Almouzni-Pettinotti +8 more
TL;DR: HJURP centromeric localization is cell cycle regulated, and its transient appearance at the centromere coincides precisely with the proposed time window for new CENP-A deposition and maintenance at centromeres.
Journal ArticleDOI
Epigenetic regulation of centromeric chromatin: old dogs, new tricks?
Robin C. Allshire,Gary H. Karpen +1 more
TL;DR: New models for how centromeric chromatin is established and propagated are proposed for the establishment and maintenance of centromere identity and kinetochore assembly.
Journal ArticleDOI
The life and miracles of kinetochores
TL;DR: This review discusses how the molecular organization of the four modules allows a dynamic integration of kinetochore–microtubule attachment with the prevention of chromosome segregation errors and cell‐cycle progression.
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TL;DR: There seems to be a slight bias against GC-rich transcripts in current gene prediction procedures in the “full-length long Japan” collection of sequenced human cDNAs.
Journal ArticleDOI
Centromeres and Kinetochores: From Epigenetics to Mitotic Checkpoint Signaling
TL;DR: Efforts to understand the nature and specification of the centromere have demonstrated that this central element for ensuring inheritance is itself epigenetically determined.