Conjugation Chemistry-Dependent T-Cell Activation with Spherical Nucleic Acids
Reads0
Chats0
TLDR
The role of peptide chemical conjugation to the DNA, which is used to load SNAs with antigens via hybridization, is explored in the context of APC activation and significantly augments the downstream T-cell response in terms of both activation and proliferation.Abstract:
Spherical nucleic acids (SNAs) can be potent sequence-specific stimulators of antigen presenting cells (APCs). When loaded with peptide antigens, they can be used to activate the immune system to train T-cells to specifically kill cancer cells. Herein, the role of peptide chemical conjugation to the DNA, which is used to load SNAs with antigens via hybridization, is explored in the context of APC activation. Importantly, though the antigen chemistry does not impede TLR-9 regulated APC activation, it significantly augments the downstream T-cell response in terms of both activation and proliferation. A comparison of three linker types, (1) noncleavable, (2) cleavable but nontraceless, and (3) traceless, reveals up to an 8-fold improvement in T-cell proliferation when the traceless linker is used. This work underscores the critical importance of the choice of conjugation chemistry in vaccine development.read more
Citations
More filters
Journal ArticleDOI
Biomaterials for vaccine-based cancer immunotherapy.
TL;DR: The rational design and clinical status of several classes of cancer vaccines are discussed along with novel biomaterial‐based delivery technologies that improve their safety and efficacy.
Journal ArticleDOI
A bi-adjuvant nanovaccine that potentiates immunogenicity of neoantigen for combination immunotherapy of colorectal cancer
Qianqian Ni,Qianqian Ni,Fuwu Zhang,Yijing Liu,Zhantong Wang,Guocan Yu,Brian Liang,Gang Niu,Ting Su,Guizhi Zhu,Guangming Lu,Longjiang Zhang,Xiaoyuan Chen +12 more
TL;DR: It is concluded that banNVs are promising to optimize personalized therapeutic neoantigen vaccines for cancer immunotherapy.
Journal ArticleDOI
Bioconjugated Oligonucleotides: Recent Developments and Therapeutic Applications.
Sébastien Benizri,Sébastien Benizri,Sébastien Benizri,Arnaud Gissot,Arnaud Gissot,Arnaud Gissot,Andrew Martin,Brune Vialet,Brune Vialet,Brune Vialet,Mark W. Grinstaff,Philippe Barthélémy,Philippe Barthélémy,Philippe Barthélémy +13 more
TL;DR: Bioconjugated oligonucleotide-based agents have the potential to treat or cure almost any disease, and are one of the key therapeutic drug classes of the future as discussed by the authors.
Journal ArticleDOI
Bioconjugated oligonucleotides: recent developments and thera-eutic applications
Sébastien Benizri,Arnaud Gissot,Andrew Martin,Brune Vialet,Mark W. Grinstaff,Philippe Barthélémy +5 more
TL;DR: This Review first briefly describes two approaches for inhibiting specific genes using oligonucleotides-antisense DNA (ASO) and RNA interference (RNAi)-followed by a discussion on delivery to cells, and summarizes the state of the field, describe current limitations, and discuss future prospects.
Journal ArticleDOI
Supramolecularly Engineered Circular Bivalent Aptamer for Enhanced Functional Protein Delivery
Ying Jiang,Ying Jiang,Xiaoshu Pan,Jin Chang,Weijia Niu,Weijia Hou,Hailan Kuai,Zilong Zhao,Ji Liu,Ming Wang,Weihong Tan,Weihong Tan,Weihong Tan +12 more
TL;DR: The design of a β-cyclodextrin-modified cb-apt (cb-apt-βCD) and its supramolecular interaction with molecular therapeutics via host-guest chemistry for targeted intracellular delivery and a general platform to expand and diversify the function of aptamers, enabling new biological and therapeutic applications.
References
More filters
Journal ArticleDOI
Dendritic cells and the control of immunity
TL;DR: Once a neglected cell type, dendritic cells can now be readily obtained in sufficient quantities to allow molecular and cell biological analysis and the realization that these cells are a powerful tool for manipulating the immune system is realized.
Journal ArticleDOI
Scanometric DNA Array Detection with Nanoparticle Probes
TL;DR: When coupled with a signal amplification method based on nanoparticle-promoted reduction of silver(I), the sensitivity of this scanometric array detection system exceeds that of the analogous fluorophore system by two orders of magnitude.
Journal ArticleDOI
Identification of a novel cell type in peripheral lymphoid organs of mice. I. Morphology, quantitation, tissue distribution.
Ralph M. Steinman,Zanvil A. Cohn +1 more
TL;DR: Dendritic cells represent a novel cell type on both functional and morphological grounds and do not possess the functional properties of other types of reticular cells proposed to exist in lymphoid organs.
Journal ArticleDOI
Oligonucleotide-Modified Gold Nanoparticles for Intracellular Gene Regulation
Nathaniel L. Rosi,David A. Giljohann,C. Shad Thaxton,Abigail K. R. Lytton-Jean,Min Su Han,Chad A. Mirkin +5 more
TL;DR: By chemically tailoring the density of DNA bound to the surface of gold nanoparticles, a tunable gene knockdown was demonstrated and it was demonstrated that gold nanoparticle-oligonucleotide complexes are nontoxic to the cells under the conditions studied.
Journal ArticleDOI
Drug delivery strategy utilizing conjugation via reversible disulfide linkages: role and site of cellular reducing activities
TL;DR: This review focuses on understanding where and how the disulfide bond in the bioconjugate is reduced upon contact with biological milieu, which affects delivery design and the interpretation of the delivery strategies.