Journal ArticleDOI
Daily coordination of orexinergic gating in the rat superior colliculus—Implications for intrinsic clock activities in the visual system
Lukasz Chrobok,Jagoda Stanislawa Jeczmien-Lazur,Monika Bubka,Kamil Pradel,Aleksandra Klekocinska,Jasmin Daniela Klich,Amalia Ridla Rahim,Jihwan Myung,Mariusz Kepczynski,Marian H. Lewandowski +9 more
TLDR
In this paper, a combination of electrophysiological, immunohistochemical, and molecular approaches across 24 hours was used to investigate the daily coordination between the orexin system and the superior colliculus (SC).Abstract:
The orexinergic system delivers excitation for multiple brain centers to facilitate behavioral arousal, with its malfunction resulting in narcolepsy, somnolence, and notably, visual hallucinations. Since the circadian clock underlies the daily arousal, a timed coordination is expected between the orexin system and its target subcortical visual system, including the superior colliculus (SC). Here, we use a combination of electrophysiological, immunohistochemical, and molecular approaches across 24 h, together with the neuronal tract-tracing methods to investigate the daily coordination between the orexin system and the rodent SC. Higher orexinergic input was found to occur nocturnally in the superficial layers of the SC, in time for nocturnal silencing of spontaneous firing in this visual brain area. We identify autonomous daily and circadian expression of clock genes in the SC, which may underlie these day-night changes. Additionally, we establish the lateral hypothalamic origin of the orexin innervation to the SC and that the SC neurons robustly respond to orexin A via OX2 receptor in both excitatory and GABAA receptor-dependent inhibitory manners. Together, our evidence elucidates the combination of intrinsic and extrinsic clock mechanisms that shape the daily function of the visual layers of the SC.read more
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Posted ContentDOI
Intrinsic circadian timekeeping properties of the thalamic lateral geniculate nucleus
Lukasz Chrobok,Kamil Pradel,Marcelina Janik,Anna M Sanetra,Monika Bubka,Jihwan Myung,Amalia Ridla Rahim,Jasmin Daniela Klich,Jagoda Stanislawa Jeczmien-Lazur,Katarzyna Palus-Chramiec,Marian H. Lewandowski +10 more
TL;DR: In this article, a combination of molecular, electrophysiological, and optogenetic tools were used to evaluate intrinsic clock properties of the lateral geniculate nucleus (LGN) in male rats and mice.
Journal ArticleDOI
Racing and Pacing in the Reward System: A Multi-Clock Circadian Control Over Dopaminergic Signalling
TL;DR: The dopaminergic system is placed as a hub in the extensive network of extra-SCN circadian oscillators and the possible consequences of its daily entrainment for animal physiology and behaviour are discussed.
Journal ArticleDOI
From Fast Oscillations to Circadian Rhythms: Coupling at Multiscale Frequency Bands in the Rodent Subcortical Visual System
TL;DR: The subcortical visual system (SVS) is a unique collection of brain structures localised in the thalamus, hypothalamus and midbrain this article, which receives ambient light inputs from retinal ganglion cells and integrates this signal with internal homeostatic demands to influence physiology.
Journal ArticleDOI
Electrophysiological complexity in the rat dorsomedial hypothalamus and its susceptibility to daily rhythms and high‐fat diet
TL;DR: This study is the first to show electrophysiological complexity of the DMH with its sensitivity to diet and daily rhythms, and provides an electrophysics characteristic of DMH neurons with a separation into three anatomically and functionally distinct subpopulations, namely, the compact part.
Journal ArticleDOI
Ticking and talking in the brainstem satiety centre: Circadian timekeeping and interactions in the diet-sensitive clock of the dorsal vagal complex
TL;DR: Evaluating how intrinsic circadian signals regulate molecular and cellular activity in the area postrema (AP), nucleus of the solitary tract (NTS), and dorsal motor nucleus ofThe vagus (DMV) of the DVC suggests a simple framework in which to model these oscillators and their interactions is suggested.
References
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TL;DR: This paper presents a meta-analyses of the determinants of earthquake-triggered landsliding in the Czech Republic over a period of 18 months in order to establish a probabilistic framework for estimating the intensity of the earthquake.
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Neurons Containing Hypocretin (Orexin) Project to Multiple Neuronal Systems
Christelle Peyron,Devin K. Tighe,Anthony N. van den Pol,Anthony N. van den Pol,Luis de Lecea,H. Craig Heller,J. Gregor Sutcliffe,Thomas S. Kilduff +7 more
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PERIOD2::LUCIFERASE real-time reporting of circadian dynamics reveals persistent circadian oscillations in mouse peripheral tissues
Seung Hee Yoo,Shin Yamazaki,Shin Yamazaki,Phillip L. Lowrey,Kazuhiro Shimomura,Caroline H. Ko,Caroline H. Ko,Ethan D. Buhr,Sandra M. Siepka,Hee Kyung Hong,Won Jun Oh,Ook Joon Yoo,Michael Menaker,Joseph S. Takahashi +13 more
TL;DR: It is demonstrated that peripheral tissues express self-sustained, rather than damped, circadian oscillations and the existence of organ-specific synchronizers of circadian rhythms at the cell and tissue level is suggested.
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