Derivation of multipotent mesenchymal precursors from human embryonic stem cells.
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TLDR
This work presents culture conditions for the derivation of unlimited numbers of pure mesenchymal precursors from human embryonic stem cells and demonstrates multilineage differentiation into fat, cartilage, bone, and skeletal muscle cells.Abstract:
Background Human embryonic stem cells provide access to the earliest stages of human development and may serve as a source of specialized cells for regenerative medicine. Thus, it becomes crucial to develop protocols for the directed differentiation of embryonic stem cells into tissue-restricted precursors. Methods and Findings Here, we present culture conditions for the derivation of unlimited numbers of pure mesenchymal precursors from human embryonic stem cells and demonstrate multilineage differentiation into fat, cartilage, bone, and skeletal muscle cells. Conclusion Our findings will help to elucidate the mechanism of mesoderm specification during embryonic stem cell differentiation and provide a platform to efficiently generate specialized human mesenchymal cell types for future clinical applications.read more
Citations
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Repairing skeletal muscle: regenerative potential of skeletal muscle stem cells
TL;DR: The main properties of satellite cells and other myogenic progenitors are discussed as well as recent efforts to obtain myogenic cells from pluripotent stem cells for patient-tailored cell therapy.
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Whole-genome mapping of histone H3 Lys4 and 27 trimethylations reveals distinct genomic compartments in human embryonic stem cells.
Xiao Dong Zhao,Xu Han,Joon Lin Chew,Joon Lin Chew,Jun Liu,Kuo Ping Chiu,Andre Choo,Yuriy L. Orlov,Wing-Kin Sung,Wing-Kin Sung,Atif Shahab,Vladimir A. Kuznetsov,Guillaume Bourque,Steve Oh,Yijun Ruan,Huck-Hui Ng,Huck-Hui Ng,Chia-Lin Wei +17 more
TL;DR: These global histone methylation maps provide an epigenetic framework that enables the discovery of novel transcriptional networks and delineation of different genetic compartments of the pluripotent cell genome.
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Isolation and directed differentiation of neural crest stem cells derived from human embryonic stem cells
Gabsang Lee,Hyesoo Kim,Yechiel Elkabetz,George Al Shamy,Georgia Panagiotakos,Tiziano Barberi,Viviane Tabar,Lorenz Studer +7 more
TL;DR: It is shown that NCS cells plated at clonal density give rise to multiple neural crest lineages and the availability of unlimited numbers of human N CS cells offers new opportunities for studies of neural crest development and for efforts to model and treat neural crest–related disorders.
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Making muscle: skeletal myogenesis in vivo and in vitro.
TL;DR: This Review provides a comprehensive overview of skeletal myogenesis from the earliest premyogenic progenitor stage to terminally differentiated myofibers, and discusses how this knowledge has been applied to differentiate PSCs into muscle fibers and their progenitors in vitro.
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Stretch-activated ion channel Piezo1 directs lineage choice in human neural stem cells
Medha M. Pathak,Jamison L. Nourse,Truc T. Tran,Jennifer Hwe,Janahan Arulmoli,Dai Trang T. Le,Elena Bernardis,Lisa A. Flanagan,Francesco Tombola +8 more
TL;DR: It is proposed that the mechanically gated ion channel Piezo1 is an important determinant of mechanosensitive lineage choice in neural stem cells and may play similar roles in other multipotent stem cells.
References
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Journal ArticleDOI
Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
TL;DR: Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
Journal ArticleDOI
Embryonic Stem Cell Lines Derived from Human Blastocysts
James A. Thomson,Joseph Itskovitz-Eldor,Sander S. Shapiro,Michelle A. Waknitz,Swiergiel Jennifer J,Vivienne S. Marshall,Jeffrey M. Jones +6 more
TL;DR: Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages.
Journal ArticleDOI
Multilineage cells from human adipose tissue: implications for cell-based therapies.
Patricia A. Zuk,Min Zhu,Hiroshi Mizuno,Jerry I. Huang,Futrell Jw,Adam J. Katz,Prosper Benhaim,H. P. Lorenz,Marc H. Hedrick +8 more
TL;DR: The data support the hypothesis that a human lipoaspirate contains multipotent cells and may represent an alternative stem cell source to bone marrow-derived MSCs.
Journal ArticleDOI
Functional expression cloning of nanog, a pluripotency sustaining factor in embryonic stem cells
Ian Chambers,Douglas Colby,Morag Robertson,Jennifer Nichols,Sonia Lee,Susan Tweedie,Austin Smith +6 more
TL;DR: These findings establish a central role for Nanog in the transcription factor hierarchy that defines ES cell identity and confirm that Cytokine dependence, multilineage differentiation, and embryo colonization capacity are fully restored upon transgene excision.
Journal ArticleDOI
In vitro differentiation of transplantable neural precursors from human embryonic stem cells
TL;DR: In vitro differentiation, enrichment, and transplantation of neural precursor cells from human ES cells are described, depicting humanES cells as a source of transplantable neural precursors for possible nervous system repair.