Open AccessJournal Article
Detection of Chlamydophila psittaci from pigeons by polymerase chain reaction in Ahvaz.
TLDR
The results suggest that Cp.Abstract:
Background and Objective : Chlamydophila psittaci is a lethal bacterium that causes endemic avian chlamydiosis, and respiratory psittacosis. Laboratory diagnosis of Chlamydophila psittaci is difficult by culture. This study was design to investigate the presence of Chlamydophila psittaci in collected pharyngeal swabs from asyptomatic pigeons by PCR. Materials and Methods : Pharyngeal samples from pigeons with no symptoms of disease (n=280) were collected during hot and cold seasons in different parts of Ahvaz. DNA was extracted from specimens and subjected to PCR targeting pmp genes and 16s-23s rRNA intergenic spacer of Cp. psittaci and chlamydiales specific primers. Results : Of 280 samples 2 (0.7%) harbor were positive for chlamydiales (16s-23s intergenic spacer) and Cp. psittaci specific genes (pmp gene). Conclusions : In this research the pigeons were asymptomatic carriers for Cp. psittaci in their respiratory discharges. These results suggest that Cp. psittaci infection of human can occur in very close and continuous contact with pigeons.read more
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References
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Journal ArticleDOI
Sequencing of the Chlamydophila psittaci ompA Gene Reveals a New Genotype, E/B, and the Need for a Rapid Discriminatory Genotyping Method
Tom Geens,Ann Desplanques,Marnix Van Loock,Brigitte M. Bönner,Erhard F. Kaleta,Simone Magnino,Arthur A. Andersen,Karin D. E. Everett,Daisy Vanrompay +8 more
TL;DR: Results reveal the presence of a new genotype, E/B, in several European countries and stress the need for a discriminatory rapid genotyping method.
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Characterization of avian Chlamydia psittaci strains using omp1 restriction mapping and serovar-specific monoclonal antibodies.
TL;DR: Genotyping enabled a more precise differentiation of avian C. psittaci serovar A strains and can thus be a highly valuable alternative to serotyping, especially when applied directly to the clinical samples.
Journal ArticleDOI
Transcriptional response patterns of Chlamydophila psittaci in different in vitro models of persistent infection.
Stefanie Goellner,Evelyn Schubert,Elisabeth M. Liebler-Tenorio,Helmut Hotzel,Hans Peter Saluz,Konrad Sachse +5 more
TL;DR: Consistent downregulation of membrane proteins, chlamydial sigma factors, cell division protein, and reticulate body-elementary body differentiation proteins from 24 h postinfection onward proved to be a general feature of C. psittaci persistence, which suggests that there is no persistence model per se.
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The ribosomal intergenic spacer and domain I of the 23S rRNA gene are phylogenetic markers for Chlamydia spp.
TL;DR: DNA sequence analysis of either the intergenic spacer or domain I provides a rapid and reproducible method for identifying, grouping, and classifying chlamydial strains.
Journal ArticleDOI
Identification of nine species of the Chlamydiaceae using PCR-RFLP.
TL;DR: A PCR method for species identification of chlamydial isolates and for distinguishing mycoplasmas and chlamydiae was created and can be used to rapidly identify known species of the family Chlamydiaceae.