Open AccessJournal Article
Detection of Chlamydophila psittaci from pigeons by polymerase chain reaction in Ahvaz.
TLDR
The results suggest that Cp.Abstract:
Background and Objective : Chlamydophila psittaci is a lethal bacterium that causes endemic avian chlamydiosis, and respiratory psittacosis. Laboratory diagnosis of Chlamydophila psittaci is difficult by culture. This study was design to investigate the presence of Chlamydophila psittaci in collected pharyngeal swabs from asyptomatic pigeons by PCR. Materials and Methods : Pharyngeal samples from pigeons with no symptoms of disease (n=280) were collected during hot and cold seasons in different parts of Ahvaz. DNA was extracted from specimens and subjected to PCR targeting pmp genes and 16s-23s rRNA intergenic spacer of Cp. psittaci and chlamydiales specific primers. Results : Of 280 samples 2 (0.7%) harbor were positive for chlamydiales (16s-23s intergenic spacer) and Cp. psittaci specific genes (pmp gene). Conclusions : In this research the pigeons were asymptomatic carriers for Cp. psittaci in their respiratory discharges. These results suggest that Cp. psittaci infection of human can occur in very close and continuous contact with pigeons.read more
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References
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Journal ArticleDOI
Frequency of Development and Associated Physiological Cost of Azithromycin Resistance in Chlamydia psittaci 6BC and C. trachomatis L2
Rachel Binet,Anthony T. Maurelli +1 more
TL;DR: The hypothesis that the mechanisms which confer high-level macrolide resistance in chlamydiae carry a prohibitive physiological cost and may thus limit the emergence of highly resistant clones of these important pathogens in vivo is supported.
Journal ArticleDOI
Use of a nested PCR-enzyme immunoassay with an internal control to detect Chlamydophila psittaci in turkeys.
Marnix Van Loock,Kristel Verminnen,Trudy O Messmer,Guido Volckaert,Bruno Goddeeris,Bruno Goddeeris,Daisy Vanrompay +6 more
TL;DR: The present study stresses the need for an internal control to confirm PCR true-negatives and demonstrates the high prevalence of chlamydiosis in Belgian turkeys and its potential zoonotic risk.
Journal ArticleDOI
Detection of chlamydiae in boar semen and genital tracts.
Komkrich Teankum,Andreas Pospischil,Fredi Janett,E. Bürgi,Enrico Brugnera,Katharina Hoelzle,Adam Polkinghorne,R. Weilenmann,Dieter R. Zimmermann,Nicole Borel +9 more
TL;DR: The prevalence of chlamydial infection was low in group A animals indicating that venereal transmission may not be significant for Chlamydia-associated reproductive diseases in pigs, although rare cases may occur.
Journal ArticleDOI
PCR detection of Chlamydia psittaci in faecal samples from passerine birds in Sweden.
TL;DR: To investigate to what extent wild passerine birds are carriers of Chlamydia psittaci, 312 faecal samples from 18 bird species were collected and results indicate that a significant proportion of wild passerines birds are carrier of C. psittacia, but rarely infectious to humans.
Journal ArticleDOI
Development of an internally controlled real-time PCR assay for detection of Chlamydophila psittaci in the LightCycler 2.0 system.
Edou R. Heddema,M.G.H.M. Beld,B. de Wever,Ankie A. J. Langerak,Yvonne Pannekoek,Birgitta Duim +5 more
TL;DR: A real-time PCR assay with a DNA purification and inhibition control (internal control; IC) was developed to detect Chlamydophila psittaci DNA in human clinical samples as mentioned in this paper.