Open AccessJournal Article
Detection of Chlamydophila psittaci from pigeons by polymerase chain reaction in Ahvaz.
TLDR
The results suggest that Cp.Abstract:
Background and Objective : Chlamydophila psittaci is a lethal bacterium that causes endemic avian chlamydiosis, and respiratory psittacosis. Laboratory diagnosis of Chlamydophila psittaci is difficult by culture. This study was design to investigate the presence of Chlamydophila psittaci in collected pharyngeal swabs from asyptomatic pigeons by PCR. Materials and Methods : Pharyngeal samples from pigeons with no symptoms of disease (n=280) were collected during hot and cold seasons in different parts of Ahvaz. DNA was extracted from specimens and subjected to PCR targeting pmp genes and 16s-23s rRNA intergenic spacer of Cp. psittaci and chlamydiales specific primers. Results : Of 280 samples 2 (0.7%) harbor were positive for chlamydiales (16s-23s intergenic spacer) and Cp. psittaci specific genes (pmp gene). Conclusions : In this research the pigeons were asymptomatic carriers for Cp. psittaci in their respiratory discharges. These results suggest that Cp. psittaci infection of human can occur in very close and continuous contact with pigeons.read more
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Bayesian evaluation of the performance of three diagnostic tests for Chlamydophila psittaci in humans
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Survey on Carrier State of Sheep in Chlamydia pecorum Infection
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References
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Journal ArticleDOI
Usefulness of omp1 restriction mapping for avian Chlamydia psittaci isolate differentiation.
Chalom Sayada,Arthur A. Andersen,C Storey,Alain Milon,F. Eb,N Hashimoto,K Hirai,Jacques Elion,Erick Denamur +8 more
TL;DR: This PCR approach based upon omp1 restriction mapping enables the differentiation of avian C. psittaci isolates and can be proposed as a taxonomic and epidemiologic tool.
Journal ArticleDOI
Improved sensitivity of PCR for Chlamydophila using pmp genes.
TL;DR: It is demonstrated that these specific pmp primers could serve as valuable, sensitive and common tools for a specific Chlamydophila diagnosis in ruminant, avian and human diseases.
Journal ArticleDOI
Identification of a multigene family coding for the 90 kDa proteins of the ovine abortion subtype of Chlamydia psittaci
TL;DR: These clones contained fragments of a gene coding for a group of three chlamydial proteins of approximately 90 kDa which appeared as major immunogens by immunoblotting experiments, indicating their potential as diagnostic or possibly protective antigens.
Journal ArticleDOI
Chlamydiosis in workers at a duck farm and processing plant.
TL;DR: An outbreak of chlamydiosis was investigated in workers at a duck farm and processing plant during winter 1989 and there was a strong association between years of employment and exposure to infection.
Journal ArticleDOI
Detection and strain differentiation of Chlamydia psittaci mediated by a two-step polymerase chain reaction.
TL;DR: Specific and sensitive amplification of major outer membrane protein (MOMP) gene DNA sequences of Chlamydia psittaci was achieved in a two-step polymerase chain reaction and restriction endonuclease analysis with HaeIII indicated a close relationship between C. Psittaci strains of avian and mammalian serovar 1 lineage.