Journal ArticleDOI
Detection of single cell heterogeneity in cancer.
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TLDR
Various detection targets applied in single cell study, including tumor tissue cells, circulating tumor cells, disseminated tumor cells (DTCs), circulating tumor DNA (ctDNA), cell-free DNA (cfDNA), and cancer stem cells (CSCs), are listed and detection methods using these detection targets in different fields to reveal single cell heterogeneity in cancer are discussed.About:
This article is published in Seminars in Cell & Developmental Biology.The article was published on 2017-04-01. It has received 50 citations till now. The article focuses on the topics: Cancer stem cell & Single cell sequencing.read more
Citations
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Journal ArticleDOI
The evolving concept of liver cancer stem cells.
TL;DR: This review focuses on the recent advances in understanding of the biology of liver CSCs and the development of strategies for their treatment.
Journal ArticleDOI
Single-cell heterogeneity in Sézary syndrome.
Terkild B. Buus,Andreas Willerslev-Olsen,Simon Fredholm,Edda Blümel,Claudia Nastasi,Maria Gluud,Tengpeng Hu,Lise M. Lindahl,Lars Iversen,Hanne Fogh,Robert Gniadecki,Ivan V. Litvinov,Jenny L. Persson,Jenny L. Persson,Charlotte M. Bonefeld,Carsten Geisler,Jan Pravsgaard Christensen,Thorbjørn Krejsgaard,Thomas Litman,Anders Woetmann,Niels Ødum +20 more
TL;DR: The presence of single-cell heterogeneity and resistance to histone deacetylase inhibitors (HDACi) within primary malignant T cells from patients with SS is investigated and it is shown that distinct subpopulations of malignantT cells carry HDACi resistance.
Journal ArticleDOI
Single-cell sequencing and tumorigenesis: improved understanding of tumor evolution and metastasis.
Darrell L. Ellsworth,Heather L. Blackburn,Craig D. Shriver,Shahrooz Rabizadeh,Patrick Soon-Shiong,Rachel E. Ellsworth +5 more
TL;DR: Current methodologies and recent technological advances for isolating single cells, single-cell whole-genome and whole-transcriptome amplification using minute amounts of nucleic acids, and SCS are highlighted and the promise for integrating SCS in the clinical care arena for improved patient care is discussed.
Journal ArticleDOI
Modeling the process of human tumorigenesis.
TL;DR: In this review, the advantages, contributions and limitations of current de novo human tumorigenesis strategies are surveyed and several exciting prospects on the horizon are noted.
Journal ArticleDOI
Tumorigenesis as a process of gradual loss of original cell identity and gain of properties of neural precursor/progenitor cells.
TL;DR: Synthesis of the information strongly supports that cancer cells share much more similarities with neural progenitor/stem cells than with mesenchymal-type cells and that tumorigenesis represents a process of gradual loss of cell or lineage identity and gain of characteristics of neural cells.
References
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Journal ArticleDOI
Single-cell RNA-seq highlights intratumoral heterogeneity in primary glioblastoma
Anoop P. Patel,Itay Tirosh,John J. Trombetta,Alex K. Shalek,Shawn M. Gillespie,Hiroaki Wakimoto,Daniel P. Cahill,Brian V. Nahed,William T. Curry,Robert L. Martuza,David N. Louis,Orit Rozenblatt-Rosen,Mario L. Suvà,Mario L. Suvà,Aviv Regev,Aviv Regev,Aviv Regev,Bradley E. Bernstein,Bradley E. Bernstein,Bradley E. Bernstein +19 more
TL;DR: The genome sequence of single cells isolated from brain glioblastomas was examined, which revealed shared chromosomal changes but also extensive transcription variation, including genes related to signaling, which represent potential therapeutic targets.
Journal ArticleDOI
Tumour evolution inferred by single-cell sequencing
Nicholas Navin,Jude Kendall,Jennifer Troge,Peter Andrews,Linda Rodgers,Jeanne McIndoo,Kerry Cook,Asya Stepansky,Dan Levy,Diane Esposito,Lakshmi Muthuswamy,Alexander Krasnitz,W. Richard McCombie,James W. Hicks,Michael Wigler +14 more
TL;DR: It is shown that with flow-sorted nuclei, whole genome amplification and next generation sequencing the authors can accurately quantify genomic copy number within an individual nucleus and indicate that tumours grow by punctuated clonal expansions with few persistent intermediates.
Journal ArticleDOI
Evolution and Impact of Subclonal Mutations in Chronic Lymphocytic Leukemia
Dan A. Landau,Scott L. Carter,Petar Stojanov,Petar Stojanov,Aaron McKenna,Kristen E. Stevenson,Michael S. Lawrence,Carrie Sougnez,Chip Stewart,Andrey Sivachenko,Lili Wang,Youzhong Wan,Wandi Zhang,Sachet A. Shukla,Sachet A. Shukla,Alexander R. Vartanov,Stacey M. Fernandes,Gordon Saksena,Kristian Cibulskis,Bethany Tesar,Stacey Gabriel,Nir Hacohen,Nir Hacohen,Matthew Meyerson,Eric S. Lander,Donna Neuberg,Jennifer R. Brown,Jennifer R. Brown,Gad Getz,Gad Getz,Catherine J. Wu,Catherine J. Wu +31 more
TL;DR: Analysis of intratumoral heterogeneity in chronic lymphocytic leukemia cases uncovers patterns of clonal evolution in CLL, providing insights into its stepwise transformation, and links the presence of subclones with adverse clinical outcomes.
Journal ArticleDOI
Non-genetic origins of cell-to-cell variability in TRAIL-induced apoptosis
TL;DR: It is shown that naturally occurring differences in the levels or states of proteins regulating receptor-mediated apoptosis are the primary causes of cell-to-cell variability in the timing and probability of death in human cell lines.
Journal ArticleDOI
Clonal evolution in breast cancer revealed by single nucleus genome sequencing
Yong Wang,Jill Waters,Marco L. Leung,Anna K. Unruh,Whijae Roh,Xiuqing Shi,Ken Chen,Paul Scheet,Selina Vattathil,Han Liang,Asha S. Multani,Hong Zhang,Rui Zhao,Franziska Michor,Funda Meric-Bernstam,Nicholas Navin +15 more
TL;DR: The data show that aneuploid rearrangements occurred early in tumour evolution and remained highly stable as the tumour masses clonally expanded, which has important implications for the diagnosis, therapeutic treatment and evolution of chemoresistance in breast cancer.