Journal ArticleDOI
Differences in the distribution of bifidobacterial and enterobacterial species in human faecal microflora of three different (children, adults, elderly) age groups
Françoise Gavini,Chantal Cayuela,Jean-Michel Antoine,Catherine Lecoq,Bernard Lefebvre,Jeanne-Marie Membré,Christel Neut +6 more
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TLDR
To determine if there is any change with age in the distribution of Bifidobacterium and Enterobacteriaceae species in human intestinal microflora, strains were isolated from a total of 54 samples of human faeces and there is a noticeable trend whereby B. adolescentis occurs more frequently in children than in the elderly.Abstract:
To determine if there is any change with age in the distribution of Bifidobacterium and Enterobacteriaceae species in human intestinal microflora, strains were isolated from a total of 54 samples of human faeces (15 children, 3–15 years old; 17 adults, 30–46 years old; 22 elderly, 69–89 years old). The bifidobacteria were identified at species level using a numerical phenotypic identification system developed on the Internet (http://kounou.lille.inra.fr) and DNA–DNA hyridization. The enterobacteria were identified using the API 20E system (Biomerieux, France). Escherichia coli was present in 93% of samples. Enterobacter and Klebsiella species were more frequently found in children while Proteus and Providencia species were typically found in the elderly. The species Bifiobacterium longum was the most frequently species isolated in children and adults, whereas Bifidobacterium adolescentis was the most encountered species in the elderly. The distribution of B. adolescentis was significantly split between the children and the elderly age groups. There is a noticeable trend whereby Bi?fidobacterium pseudocatenulatum occurs more frequently in children than in the elderly. The species Bifidobacterium infantis , Bi?fidobacterium gallicum and Bi?fidobacterium angulatum have never been isolated, while Bi?fidobacterium breve has only been isolated on one occasion. Keywords : human faeces, elderly, Bi?fidobacterium longum , Bi?fidobacterium adolescentis , Bi?fidobacterium pseudocatenulatum , Bi?fidobacterium dentium , Escherichia coli , Enterobacter , Klebsiella , Proteus , Providencia .read more
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Bacterial metabolism and health-related effects of galacto-oligosaccharides and other prebiotics
TL;DR: Inulin-derived oligosaccharides and GOS are mildly laxative, but can result in flatulence and osmotic diarrhoea if taken in large amounts.
Journal ArticleDOI
Characterization of Bacterial Communities in Feces from Healthy Elderly Volunteers and Hospitalized Elderly Patients by Using Real-Time PCR and Effects of Antibiotic Treatment on the Fecal Microbiota
TL;DR: The numbers of enterobacteria increased in the hospitalized patients who did not receive antibiotics, and due to profound changes in fecal microbiotas during antibiotic treatment, the opportunistic species Enterococcus faecalis proliferated.
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The Lactobacillus and Bifidobacterium microflora of the human intestine: composition and succession.
TL;DR: The autochthonous Lactobacillus and Bifidobacterium microflora in man will remain stable life-long, however, some successions may be caused by transient species derived from food or from the oral cavity, thus giving the impression of an altered microFLora.
Journal ArticleDOI
Intestinal bacteria and ageing
TL;DR: Dietary supplements containing prebiotics, probiotics and a combination of both of these, synbiotics show promising results with these supplements, although further longer‐term investigations are required to substantiate their use in elderly healthcare fields.
Journal ArticleDOI
Gut microbiome and aging: Physiological and mechanistic insights
Ravinder Nagpal,Rabina Mainali,Shokouh Ahmadi,Shokouh Ahmadi,Shaohua Wang,Ria Singh,Kylie Kavanagh,Dalane W. Kitzman,Almagul Kushugulova,Francesco Marotta,Hariom Yadav +10 more
TL;DR: The present review summarizes the evidences related to the age-associated changes in intestinal microbiota and vice-versa, mechanisms involved in this bi-directional relationship, and the prospective for development of microbiota-based interventions such as probiotics for healthy aging.
References
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Journal ArticleDOI
A procedure for the isolation of deoxyribonucleic acid from micro-organisms
TL;DR: A method has been described for the isolation of DNA from micro-organisms which yields stable, biologically active, highly polymerized preparations relatively free from protein and RNA, and Representative samples have been characterized for their thermal stability and sedimentation behaviour.
Journal ArticleDOI
The quantitative measurement of DNA hybridization from renaturation rates.
TL;DR: A new method is proposed to measure relatedness amongst bacteria, based on renaturation rate determinations of DNA types and their mixture, and the advantages are summarized.
Journal ArticleDOI
The Intestinal Microflora of Infants: Composition of Fecal Flora in Breast-Fed and Bottle-Fed Infants
TL;DR: The fecal flora of 35 breast‐fed and 35 bottle‐fed babies was determined and the organism that showed the highest number and the highest frequency of occurrence in both groups was Bifidobacterium breve.
Journal ArticleDOI
Modification of the Intestinal Microflora Using Probiotics and Prebiotics
R. Fuller,Glenn R. Gibson +1 more
TL;DR: Probiotics and prebiotics modulate the composition of the human gut microbiota and represent an attempt to reconstitute the gut flora to its normal composition which has been adversely affected by dietary and environmental stresses.
Journal ArticleDOI
An elective and selective isolation medium for Bifidobacterium spp.
TL;DR: A modified Columbia agar medium, containing 5 or 10 ml/l propionic acid and adjusted to pH 5.0, is described, which is both elective and selective for bifidobacteria.
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