Direct and sex-specific stimulation by sex steroids of creatine kinase activity and DNA synthesis in rat bone.
TLDR
A direct in vitro effect of 17 beta-estradiol (E2) was demonstrated on bone and cartilage cell energy metabolism and it is possible that direct sex-specific actions of gonadal steroids may contribute to stimulating bone growth and maintaining balanced bone turnover.Abstract:
A direct in vitro effect of 17 beta-estradiol (E2) was demonstrated on bone and cartilage cell energy metabolism. Sex-specific stimulation by E2 and testosterone was shown in diaphyseal bone of weanling rats. E2 (30 nM) caused, within 24 hr, a 70-200% increase in creatine kinase (CK; ATP:creatine N-phosphotransferase, EC 2.7.3.2) specific activity in ROS 17/2.8 rat osteogenic sarcoma cells, MC3T3-E1 mouse calvaria-derived cells, and rat fetal calvaria cells, and a 40% increase in rat epiphyseal cartilage cells. Stimulation of CK activity by E2 was dose and time dependent: in ROS 17/2.8 cells, a highly significant increase was found at 3 nM E2 and a greater than 100% increase in CK activity was found 1 hr after E2 administration. In female 20-day-old Wistar-derived rats, E2 (5 micrograms per rat) increased CK activity in diaphyseal bone by 82% within 1 hr of i.p. injection, with a maximal increase of 200% after 24 hr; neither the weakly estrogenic agonist 17 alpha-estradiol, testosterone, nor progesterone showed this effect. Conversely, in male rat diaphyseal bone, testosterone or dihydrotestosterone increased CK activity after 24 hr by approximately 100%, while E2 was ineffective. In epiphyseal cartilage, both E2 and testosterone increased CK activity. Stimulation of CK activity by sex hormones was paralleled by significant increases in [3H]thymidine incorporation into DNA. Therefore, it is possible that direct sex-specific actions of gonadal steroids may contribute to stimulating bone growth and maintaining balanced bone turnover.read more
Citations
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Journal ArticleDOI
Androgens and bone
Dirk Vanderschueren,Liesbeth Vandenput,Steven Boonen,Marie K. Lindberg,Roger Bouillon,Claes Ohlsson +5 more
TL;DR: Observations in androgen-resistant animals clearly demonstrated that the sexual dimorphism of bone depends on the presence of a functional androgen receptor, and optimal peak bone mass seems related to an appropriately timed androgen secretion.
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Creatine kinase in non-muscle tissues and cells
Theo Wallimann,Wolfram Hemmer +1 more
TL;DR: New findings concerning the isoenzyme-specific cellular localization and subcellular compartmentation of CK isoenzymes in photoreceptor cells, in glial and neuronal cells of the cerebellum and in spermatozoa are reported on.
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Estrogen-like activity of glabrene and other constituents isolated from licorice root.
TL;DR: This is the first study to indicate that glabrene, an isoflavene exerted varying degrees of ER agonism in different tissues, and both showed an ER-dependent growth-promoting effect at low concentrations, and ER-independent antiproliferative activity at concentrations >15 microM.
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Snait Tamir,Mark Eizenberg,Dalia Somjen,Naftali Stern,Rayah Shelach,Alvin M. Kaye,Jacob Vaya +6 more
TL;DR: This is the first study to indicate that isoflavans have estrogen-like activities, and glabridin and its derivatives exhibited varying degrees of estrogen receptor agonism in different tests and demonstrated growth-inhibitory actions on breast cancer cells.
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Bioenergetics and mitochondrial transmembrane potential during differentiation of cultured osteoblasts.
TL;DR: Progressive differentiation coincided with changes in cellular metabolism and mitochondrial activity, which are likely to play key roles in osteoblast function.
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