Journal ArticleDOI
Estrogen binding, receptor mRNA, and biologic response in osteoblast-like osteosarcoma cells
Barry S. Komm,Christopher M. Terpening,David J. Benz,Kimberlie A. Graeme,Alfred Gallegos,Murray Korc,Geoffrey L. Greene,Bert W. O'Malley,Mark R. Haussler +8 more
TLDR
Estradiol can act directly on osteoblasts by a receptor-mediated mechanism and thereby modulate the extracellular matrix and other proteins involved in the maintenance of skeletal mineralization and remodeling.Abstract:
High specific activity estradiol labeled with iodine-125 was used to detect approximately 200 saturable, high-affinity (dissociation constant approximately equal to 1.0 nM) nuclear binding sites in rat (ROS 17/2.8) and human (HOS TE85) clonal osteoblast-like osteosarcoma cells. Of the steroids tested, only testosterone exhibited significant cross-reactivity with estrogen binding. RNA blot analysis with a complementary DNA probe to the human estrogen receptor revealed putative receptor transcripts of 6 to 6.2 kilobases in both rat and human osteosarcoma cells. Type I procollagen and transforming growth factor-beta messenger RNA levels were enhanced in cultured human osteoblast-like cells treated with 1 nM estradiol. Thus, estrogen can act directly on osteoblasts by a receptor-mediated mechanism and thereby modulate the extracellular matrix and other proteins involved in the maintenance of skeletal mineralization and remodeling.read more
Citations
More filters
Journal ArticleDOI
Estrogen receptor null mice: what have we learned and where will they lead us?
John F. Couse,Kenneth S. Korach +1 more
TL;DR: The recent successful generation of double knockout, or alpha beta ERKO mice of both sexes, suggests that this receptor is also not essential to survival and was most likely not a compensatory factor in the survival of the alpha ERKO.
Journal ArticleDOI
Mechanisms of Estrogen Action
Stefan Nilsson,Sari Mäkelä,Eckardt Treuter,Michel Tujague,Jane S. Thomsen,Göran Andersson,Eva Enmark,Katarina Pettersson,Margaret Warner,Jan-Åke Gustafsson +9 more
TL;DR: The role of estrogen receptors in physiology and pathology has been investigated in the past decade and it was found that there was not one but two distinct and functional estrogen receptors, now called ERα and ERβ.
Journal ArticleDOI
Sex steroids and the construction and conservation of the adult skeleton.
TL;DR: A new unitary model for the pathophysiology of involutional osteoporosis is reviewed and extended that identifies estrogen (E) as the key hormone for maintaining bone mass and E deficiency as the major cause of age-related bone loss in both sexes.
Journal ArticleDOI
Minireview: The OPG/RANKL/RANK system
TL;DR: The identification of the OPG-ligand/osteoclast differentiating factor and subsequently RANKL, was identified as the key mediator of osteoclastogenesis in both a membrane-bound form expressed on preosteoblastic/stromal cells as well as a soluble form.
Journal ArticleDOI
Estrogen receptors and human disease
TL;DR: This Review will describe diseases in which estrogen plays a role in the development or severity of disease, through the ER, which serves as the basis for many therapeutic interventions.
References
More filters
Journal ArticleDOI
Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
TL;DR: A simple and rapid method for transferring RNA from agarose gels to nitrocellulose paper for blot hybridization has been developed, allowing removal of the hybridized probes and rehybridization of the RNA blots without loss of sensitivity.
Journal ArticleDOI
Purification of Biologically Active Globin Messenger RNA by Chromatography on Oligothymidylic acid-Cellulose
Haim Aviv,Philip Leder +1 more
TL;DR: A convenient technique for the partial purification of large quantities of functional, poly(adenylic acid)-rich mRNA is described and should prove generally useful as an initial step in the isolation of specific mRNAs.
Journal ArticleDOI
Human transforming growth factor-beta complementary DNA sequence and expression in normal and transformed cells.
Rik Derynck,Julie A. Jarrett,Ellson Y. Chen,Dennis H. Eaton,John Richard Bell,Richard K. Assoian,Anita B. Roberts,Michael B. Sporn,David V. Goeddel +8 more
TL;DR: The cDNA sequence indicates that the 112-amino acid monomeric form of the natural TGF-β homodimer is derived proteolytically from a much longer precursor polypeptide which may be secreted.
Journal ArticleDOI
Monoclonal antibodies localize oestrogen receptor in the nuclei of target cells.
TL;DR: The development of an immunocytochemical procedure suitable for localizing oestrophilin directly in frozen tissue sections or cells from human and several non-human sources is reported.
Journal ArticleDOI
Sequence and expression of human estrogen receptor complementary DNA
TL;DR: A complementary DNA clone containing the entire translated portion of the messenger RNA for the estrogen receptor from MCF-7 human breast cancer cells was sequenced and then expressed in Chinese hamster ovary cells to give a functional protein, which suggests that steroid receptor genes and the avian erythroblastosis viral oncogene are derived from a common primordial gene.