Distribution and Genetic Profiles of Campylobacter in Commercial Broiler Production from Breeder to Slaughter in Thailand.
Sakaoporn Prachantasena,Petcharatt Charununtakorn,Suthida Muangnoicharoen,Luck Hankla,Natthaporn Techawal,Prapansak Chaveerach,Pravate Tuitemwong,Nipa Chokesajjawatee,Nicola J. Williams,Tom J. Humphrey,Taradon Luangtongkum +10 more
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TLDR
Genotypic identification of C. jejuni in slaughterhouses indicated that broilers were the main source of Campylobacter contamination of chicken meat during processing, and control and prevention strategies should be aimed at both farm and slaughterhouse levels.Abstract:
Poultry and poultry products are commonly considered as the major vehicle of Campylobacter infection in humans worldwide. To reduce the number of human cases, the epidemiology of Campylobacter in poultry must be better understood. Therefore, the objective of the present study was to determine the distribution and genetic relatedness of Campylobacter in the Thai chicken production industry. During June to October 2012, entire broiler production processes (i.e., breeder flock, hatchery, broiler farm and slaughterhouse) of five broiler production chains were investigated chronologically. Representative isolates of C. jejuni from each production stage were characterized by flaA SVR sequencing and multilocus sequence typing (MLST). Amongst 311 selected isolates, 29 flaA SVR alleles and 17 sequence types (STs) were identified. The common clonal complexes (CCs) found in this study were CC-45, CC-353, CC-354 and CC-574. C. jejuni isolated from breeders were distantly related to those isolated from broilers and chicken carcasses, while C. jejuni isolates from the slaughterhouse environment and meat products were similar to those isolated from broiler flocks. Genotypic identification of C. jejuni in slaughterhouses indicated that broilers were the main source of Campylobacter contamination of chicken meat during processing. To effectively reduce Campylobacter in poultry meat products, control and prevention strategies should be aimed at both farm and slaughterhouse levels.read more
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Campylobacter jejuni survival in a poultry processing plant environment
Lourdes García-Sánchez,Beatriz Melero,Isabel Jaime,Marja-Liisa Hänninen,Mirko Rossi,Jordi Rovira +5 more
TL;DR: Whole genome MLST strongly suggest that highly clonal populations of C. jejuni can survive in adverse environmental conditions, even after cleaning and disinfection, and persist for longer periods than previous thought in the poultry plant environment.
Journal ArticleDOI
Prevalence, antimicrobial resistance and genetic diversity of Campylobacter coli and Campylobacter jejuni in Ecuadorian broilers at slaughter age.
Christian Vinueza-Burgos,Magali Wautier,Delphine Martiny,Marco Cisneros,Inge Van Damme,Lieven De Zutter +5 more
TL;DR: Investigation of the prevalence, antimicrobial resistance and genetic diversity of C. coli and C. jejuni in broilers from Ecuador found the resistance pattern tetracycline, ciprofloxin, and nalidixic acid was the dominant one in both Campylobacter species.
Journal ArticleDOI
Local genes for local bacteria: evidence of allopatry in the genomes of transatlantic Campylobacter populations.
Ben Pascoe,Guillaume Méric,Koji Yahara,Koji Yahara,Helen M. L. Wimalarathna,Susan Murray,Matthew D. Hitchings,E. L. Sproston,Catherine D. Carrillo,Eduardo N. Taboada,Kerry K. Cooper,Steven Huynh,Alison J. Cody,Keith A. Jolley,Martin C. J. Maiden,Noel D. McCarthy,Noel D. McCarthy,Xavier Didelot,Craig T. Parker,Samuel K. Sheppard,Samuel K. Sheppard +20 more
TL;DR: This work investigated biogeographical variation in highly recombining genes to determine the extent of clustering between genomes from geographically distinct Campylobacter populations and identified regions that have frequently and recently recombined to test their correlation with geographical origin.
Journal ArticleDOI
Dairy Cattle, a Potential Reservoir of Human Campylobacteriosis: Epidemiological and Molecular Characterization of Campylobacter jejuni From Cattle Farms.
Jae-Uk An,Hungwui Ho,Jong-Hyun Kim,Woohyun Kim,Junhyung Kim,Soo Min Lee,Seung-Hyun Mun,Jae-Ho Guk,Sahyun Hong,Seongbeom Cho +9 more
TL;DR: The farm environment influences the presence and survival of C. jejuni, which may play an important role in cycles of cattle re-infection, and dairy cattle represent potential reservoirs of human campylobacteriosis, and environmental management practices could be implemented on cattle farms to reduce the shedding.
Journal ArticleDOI
Characterization and Prevalence of Campylobacter spp. From Broiler Chicken Rearing Period to the Slaughtering Process in Eastern China.
Yuanyue Tang,Qidong Jiang,Haiyan Tang,Zhenyu Wang,Yi Yin,Fangzhe Ren,Linghua Kong,Xinan Jiao,Jinlin Huang +8 more
TL;DR: A high prevalence of Campylobacter is observed during the early chicken meat production, and further studies will be needed to investigate the diversity and transmission of Campyobacter in the poultry production chain.
References
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Journal ArticleDOI
MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0
TL;DR: An advanced version of the Molecular Evolutionary Genetics Analysis software, which currently contains facilities for building sequence alignments, inferring phylogenetic histories, and conducting molecular evolutionary analysis, is released, which enables the inference of timetrees, as it implements the RelTime method for estimating divergence times for all branching points in a phylogeny.
Brief Communication MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0
TL;DR: The Molecular Evolutionary Genetics Analysis (MEGA) software as discussed by the authors provides facilities for building sequence alignments, inferring phylogenetic histories, and conducting molecular evolutionary analysis, including the inference of timetrees.
Journal ArticleDOI
BIGSdb: Scalable analysis of bacterial genome variation at the population level
TL;DR: The Bacterial Isolate Genome Sequence Database (BIGSDB) represents a freely available resource that will assist the broader community in the elucidation of the structure and function of bacteria by means of a population genomics approach.
Journal ArticleDOI
Global Epidemiology of Campylobacter Infection
TL;DR: Overall, campylobacteriosis is still one of the most important infectious diseases that is likely to challenge global health in the years to come.
Journal ArticleDOI
Multilocus Sequence Typing System for Campylobacter jejuni
Kate E. Dingle,Frances M. Colles,David R. A. Wareing,Roisin Ure,Andrew J. Fox,F. E. Bolton,H. J. Bootsma,Rob J. L. Willems,Rachel Urwin,Martin C. J. Maiden +9 more
TL;DR: A multilocus sequence typing (MLST) system for this organism is described, which exploits the genetic variation present in seven housekeeping loci to determine the genetic relationships among isolates and indicates that C. jejuni is genetically diverse, with a weakly clonal population structure.