Ectopic expression of cone-specific G-protein-coupled receptor kinase GRK7 in zebrafish rods leads to lower photosensitivity and altered responses
Fivos Vogalis,Toshiyuki Shiraki,Daisuke Kojima,Yasutaka Wada,Yuko Nishiwaki,Jaakko Jarvinen,Jaakko Jarvinen,J Sugiyama,Koichi Kawakami,Koichi Kawakami,Ichiro Masai,Satoru Kawamura,Yoshitaka Fukada,Trevor D. Lamb +13 more
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TLDR
By engineering rod cells in the zebrafish retina that additionally express the cone variant, GRK7, it is found that they are less sensitive to light than normal rods, and evidence is found to suggest that the size of the cell's response to a single photon is normal when recovery is mediated by GRK1, but is small when mediated byGRK7.Abstract:
Non-technical summary When rod and cone photoreceptors in the eye respond to light, they need to recover, and the first step in recovery involves a protein called G-protein receptor kinase (GRK). Rods, which underlie night vision, employ a variant called GRK1, whereas cones, which mediate day vision, typically employ a variant called GRK7. We have engineered rod cells in the zebrafish retina that additionally express the cone variant, GRK7. By recording electrically from these modified rods, we have found that they are less sensitive to light than normal rods, in that regard mimicking cones. We have also found evidence to suggest that the size of the cell's response to a single photon (the smallest particle of light) is normal when recovery is mediated by GRK1, but is small (and hence somewhat cone-like) when mediated by GRK7. These results help us understand the differences between rod and cone photoreceptors.read more
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Journal ArticleDOI
Evolution of phototransduction, vertebrate photoreceptors and retina.
TL;DR: Evidence is reviewed from a wide range of studies relevant to the evolution of vertebrate photoreceptors and phototransduction, in order to permit the synthesis of a scenario for the major steps that occurred during the Evolution of cones, rods and the vertebrate retina.
BookDOI
G Protein-Coupled Receptor Kinases
TL;DR: This work focused on retinal GRKs, or photopigment kinases, rhodopsin kinase (GRK1), and GRK7, in the context of major general advances in the GRK fi eld, and decoding of the molecular basis of GRK activation and interactions with GPCR substrates, as well as theGRK interactions with cellular membranes and inhibitors.
Journal ArticleDOI
Cone visual pigments.
TL;DR: The uniqueness of cone visual pigments is shown by comparison of their molecular properties with those of non-visual opsins and rhodopsin.
Journal ArticleDOI
A Cambrian origin for vertebrate rods
TL;DR: The results make convergent evolution in the jawless and jawed vertebrate lines unlikely and indicate an early origin of rods, implying strong selective pressure toward dim-light vision in Cambrian ecosystems.
Journal ArticleDOI
Ciliary genes arl13b, ahi1 and cc2d2a differentially modify expression of visual acuity phenotypes but do not enhance retinal degeneration due to mutation of cep290 in zebrafish.
Emma M. Lessieur,Ping Song,Gabrielle C Nivar,Ellen Piccillo,Joseph Fogerty,Richard Rozic,Brian D Perkins,Brian D Perkins +7 more
TL;DR: The cep290fh 297/fh297 mutant is a useful model to study the role of genetic modifiers on photoreceptor degeneration in zebrafish and to explore how progressive photorecept degeneration influences regeneration in adult zebra fish.
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