Elasticity Maps of Living Neurons Measured by Combined Fluorescence and Atomic Force Microscopy
Reads0
Chats0
TLDR
In this article, the authors combine atomic force microscopy-based force spectroscopy with fluorescence microscopy to produce high-resolution elasticity maps for three different types of live neuronal cells: cortical (embryonic rat), embryonic chick dorsal root ganglion, and P-19 (mouse embryonic carcinoma stem cells) neurons.About:
This article is published in Biophysical Journal.The article was published on 2012-09-05 and is currently open access. It has received 131 citations till now. The article focuses on the topics: Elasticity (economics).read more
Citations
More filters
Journal ArticleDOI
Agarose-based biomaterials for tissue engineering.
Payam Zarrintaj,Saeed Manouchehri,Zahed Ahmadi,Mohammad Reza Saeb,Aleksandra M. Urbanska,David L. Kaplan,Masoud Mozafari +6 more
TL;DR: The scope of this review is to summarize the extensive researches addressing agarose-based biomaterials in order to provide an in-depth understanding of its tissue engineering-related applications.
Journal ArticleDOI
CNS Cell Distribution and Axon Orientation Determine Local Spinal Cord Mechanical Properties
David E. Koser,David E. Koser,Emadaldin Moeendarbary,Janina Hanne,Stefanie Kuerten,Stefanie Kuerten,Kristian Franze +6 more
TL;DR: A phenomenological model to estimate local mechanical properties of central nervous system (CNS) tissue is developed, which may ultimately help predicting local tissue stiffness, and hence cell behavior in response to mechanical signaling under physiological and pathological conditions, purely based on histological data.
Journal ArticleDOI
Systematic profiling of spatiotemporal tissue and cellular stiffness in the developing brain
TL;DR: The method profiles the stiffness of living tissue and cells with defined characteristics and can therefore be utilized to further understand the role of stiffness as a physical factor that determines cell fate during the formation of the cerebral cortex and other tissues.
Journal ArticleDOI
Agarose-Based Biomaterials: Opportunities and Challenges in Cartilage Tissue Engineering.
Mohammad Amin Salati,Javad Khazai,Amir Mohammad Tahmuri,Ali Samadi,Ali Taghizadeh,Mohsen Taghizadeh,Payam Zarrintaj,Josh D. Ramsey,Sajjad Habibzadeh,Farzad Seidi,Mohammad Reza Saeb,Masoud Mozafari +11 more
TL;DR: The lack of adequate blood/lymphatic vessels as well as low-potential articular cartilage regeneration underlines the necessity to search for alternative biomaterials for restoration of injured cartilage tissue.
Journal ArticleDOI
Printed Microelectrode Arrays On Soft Materials: From PDMS to Hydrogels
Nouran Adly,Nouran Adly,Sabrina Weidlich,Silke Seyock,Fabian Brings,Alexey Yakushenko,Andreas Offenhäusser,Bernhard Wolfrum,Bernhard Wolfrum +8 more
TL;DR: A cost-effective and simple approach to make soft microelectrode arrays has been developed using inkjet printing of carbon-based conductive ink, which allows for rapid prototyping of disposable sensor array structures on various soft substrates for in vitro as well as in vivo applications.
References
More filters
Journal ArticleDOI
Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
Journal ArticleDOI
Neurons derived from radial glial cells establish radial units in neocortex
Stephen C. Noctor,Alexander C. Flint,Tamily A. Weissman,Ryan S. Dammerman,Arnold R. Kriegstein +4 more
TL;DR: The results support the concept that a lineage relationship between neurons and proliferative radial glia may underlie the radial organization of neocortex.
Journal ArticleDOI
Molecular Mechanisms of Axon Guidance
TL;DR: This work has shown that a relatively small number of guidance factors can be used to generate intricate patterns of neuronal wiring through signaling pathways still only poorly understood.
Journal ArticleDOI
Molecular mechanisms of axon guidance.
TL;DR: It is becoming apparent that molecules previously known for their role in patterning can also direct axonal outgrowth and a sophisticated and dynamic set of cues that enable a growth cone to successfully navigate to its destination, modulating its response to changing environmental cues along its pathway.
Journal ArticleDOI
Cytoskeletal dynamics and transport in growth cone motility and axon guidance
Erik W. Dent,Frank B. Gertler +1 more
TL;DR: A working model for cytoskeletal regulation of directed axon outgrowth is presented and an important goal for the future will be to understand the coordinated response of the cytoskeleton to signaling cascades induced by guidance receptor activation.