Elemental formula annotation of polar and lipophilic metabolites using 13C, 15N and 34S isotope labelling, in combination with high-resolution mass spectrometry
Patrick Giavalisco,Yan Li,Annemarie Matthes,Aenne Eckhardt,Hans-Michael Hubberten,Holger Hesse,Shruthi Segu,Jan Hummel,Karin Köhl,Lothar Willmitzer +9 more
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TLDR
A comprehensive multi-isotope labelling-based strategy using fully labelled plant tissues, in combination with a fractionated metabolite extraction protocol, which can be applied in either an automated database-dependent or a database-independent analysis of the plant polar metabolome and lipidome.Abstract:
The unbiased and comprehensive analysis of metabolites in any organism presents a major challenge if proper peak annotation and unambiguous assignment of the biological origin of the peaks are required. Here we provide a comprehensive multi-isotope labelling-based strategy using fully labelled (13) C, (15) N and (34) S plant tissues, in combination with a fractionated metabolite extraction protocol. The extraction procedure allows for the simultaneous extraction of polar, semi-polar and hydrophobic metabolites, as well as for the extraction of proteins and starch. After labelling and extraction, the metabolites and lipids were analysed using a high-resolution mass spectrometer providing accurate MS and all-ion fragmentation data, providing an unambiguous readout for every detectable isotope-labelled peak. The isotope labelling assisted peak annotation process employed can be applied in either an automated database-dependent or a database-independent analysis of the plant polar metabolome and lipidome. As a proof of concept, the developed methods and technologies were applied and validated using Arabidopsis thaliana leaf and root extracts. Along with a large repository of assigned elemental compositions, which is provided, we show, using selected examples, the accuracy and reliability of the developed workflow.read more
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Glandular trichomes: what comes after expressed sequence tags?
TL;DR: Glandular trichomes cover the surface of many plant species and exhibit tremendous diversity, be it in their shape or the compounds they secrete as discussed by the authors, which has spurred research into the biosynthesis pathways that lead to these specialized metabolites.
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Systemic Analysis of Inducible Target of Rapamycin Mutants Reveal a General Metabolic Switch Controlling Growth in Arabidopsis thaliana
Camila Caldana,Yan Li,Andrea Leisse,Yi Zhang,Lisa Bartholomaeus,Alisdair R. Fernie,Lothar Willmitzer,Patrick Giavalisco +7 more
TL;DR: A role for harnessing the plant TOR pathway by utilizing it as a potent metabolic switch is proposed, offering a possible route for biotechnological optimization of plant energy content and carbon partitioning for the production of bioenergy.
Journal ArticleDOI
The Structure and Function of Major Plant Metabolite Modifications.
TL;DR: Both technical and functional aspects focusing on the influence that various modifications have on biosynthesis, degradation, transport, and storage of metabolites, as well as their bioactivity and toxicity are covered.
Journal ArticleDOI
Proteaceae from severely phosphorus-impoverished soils extensively replace phospholipids with galactolipids and sulfolipids during leaf development to achieve a high photosynthetic phosphorus-use-efficiency.
Hans Lambers,Gregory R. Cawthray,Patrick Giavalisco,John Kuo,Etienne Laliberté,Stuart J. Pearse,Wolf-Rüdiger Scheible,Mark Stitt,François P. Teste,Benjamin L. Turner,Benjamin L. Turner +10 more
TL;DR: Results clearly show that a low investment in phospholipid levels, relative to nonphospholipids, offers a partial explanation for a high photosynthetic rate per unit leaf P in Proteaceae adapted to P-impoverished soils.
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Growth of E. coli on formate and methanol via the reductive glycine pathway.
Seohyoung Kim,Steffen N. Lindner,Selçuk Aslan,Oren Yishai,Sebastian Wenk,Karin Schann,Arren Bar-Even +6 more
TL;DR: The central carbon metabolism of Escherichia coli is redesigned with the reductive glycine pathway to enable growth on the one-carbon compounds formate and CO2, and the addition of methanol dehydrogenase further enables growth on meethanol andCO2.
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